β-Cyclodextrin-Modified Magnetic Nanoparticles Immobilized on Sepharose Surface Provide an Effective Matrix for Protein Refolding

2018 ◽  
Vol 122 (43) ◽  
pp. 9907-9919 ◽  
Author(s):  
Marziyeh Ghaeidamini ◽  
Ali N. Kharat ◽  
Thomas Haertlé ◽  
Faizan Ahmad ◽  
Ali A. Saboury
Keyword(s):  
Magnetic Nanoparticles ◽  
Protein Refolding ◽  
Effective Matrix ◽  
Modified Magnetic Nanoparticles

scholarly journals Polymer Brush-Modified Magnetic Nanoparticles for His-Tagged Protein Purification

Langmuir ◽  
2011 ◽  
Vol 27 (6) ◽  
pp. 3106-3112 ◽  
Author(s):  
Fei Xu ◽  
James H. Geiger ◽  
Gregory L. Baker ◽  
Merlin L. Bruening
Keyword(s):  
Magnetic Nanoparticles ◽  
Protein Purification ◽  
Polymer Brush ◽  
Modified Magnetic Nanoparticles

scholarly journals Selective Capture and Identification of Methicillin-Resistant Staphylococcus aureus by Combining Aptamer-Modified Magnetic Nanoparticles and Mass Spectrometry

2021 ◽  
Vol 22 (12) ◽  
pp. 6571
Author(s):  
Yu-Chen Liu ◽  
Katragunta Kumar ◽  
Cheng-Hsiu Wu ◽  
Kai-Chih Chang ◽  
Cheng-Kang Chiang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Staphylococcus Aureus ◽  
Magnetic Nanoparticles ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Capture Rate ◽  
Mass Spectrometry Analysis ◽  
Ionization Mass ◽  
Methicillin Resistant ◽  
Spectrometry Analysis ◽  
Modified Magnetic Nanoparticles

A nucleic acid aptamer that specifically recognizes methicillin-resistant Staphylococcus aureus (MRSA) has been immobilized on magnetic nanoparticles to capture the target bacteria prior to mass spectrometry analysis. After the MRSA species were captured, they were further eluted from the nanoparticles and identified using matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). The combination of aptamer-based capture/enrichment and MS analysis of microorganisms took advantage of the selectivity of both techniques and should enhance the accuracy of MRSA identification. The capture and elution efficiencies for MRSA were optimized by examining factors such as incubation time, temperature, and elution solvents. The aptamer-modified magnetic nanoparticles showed a capture rate of more than 90% under the optimized condition, whereas the capture rates were less than 11% for non-target bacteria. The as-prepared nanoparticles exhibited only a 5% decrease in the capture rate and a 9% decrease in the elution rate after 10 successive cycles of utilization. Most importantly, the aptamer-modified nanoparticles revealed an excellent selectivity towards MRSA in bacterial mixtures. The capture of MRSA at a concentration of 102 CFU/mL remained at a good percentage of 82% even when the other two species were at 104 times higher concentration (106 CFU/mL). Further, the eluted MRSA bacteria were successfully identified using MALDI mass spectrometry.

Intracellular accumulation and immunological responses of lipid modified magnetic iron nanoparticles in mouse antigen processing cells

2017 ◽  
Vol 5 (8) ◽  
pp. 1603-1611 ◽  
Author(s):  
Chenmeng Qiao ◽  
Jun Yang ◽  
Lei Chen ◽  
Jie Weng ◽  
Xin Zhang
Keyword(s):  
Magnetic Nanoparticles ◽  
Immune Responses ◽  
Antigen Processing ◽  
Iron Nanoparticles ◽  
Intracellular Accumulation ◽  
Immunological Responses ◽  
Modified Magnetic Nanoparticles

Lipid modified magnetic nanoparticles could enhance the intracellular accumulation and immune responses of mouse antigen processing cells.

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