scholarly journals Ab Initio QM/MM Modeling of the Rate-Limiting Proton Transfer Step in the Deamination of Tryptamine by Aromatic Amine Dehydrogenase

2017 ◽  
Vol 121 (42) ◽  
pp. 9785-9798 ◽  
Author(s):  
Kara E. Ranaghan ◽  
William G. Morris ◽  
Laura Masgrau ◽  
Kittusamy Senthilkumar ◽  
Linus O. Johannissen ◽  
...  
Keyword(s):  
Proton Transfer ◽  
Ab Initio ◽  
Aromatic Amine ◽  
Transfer Step ◽  
Amine Dehydrogenase ◽  
Rate Limiting

scholarly journals The enzyme aromatic amine dehydrogenase induces a substrate conformation crucial for promoting vibration that significantly reduces the effective potential energy barrier to proton transfer

2008 ◽  
Vol 5 (suppl_3) ◽  
pp. 225-232 ◽  
Author(s):  
Linus O Johannissen ◽  
Nigel S Scrutton ◽  
Michael J Sutcliffe
Keyword(s):  
Potential Energy ◽  
Proton Transfer ◽  
Aromatic Amine ◽  
Effective Potential ◽  
Energy Barrier ◽  
Transfer Event ◽  
Effective Potential Energy ◽  
Potential Energy Barrier ◽  
Mechanical Methods ◽  
Amine Dehydrogenase

The role of promoting vibrations in enzymic reactions involving hydrogen tunnelling is contentious. While models incorporating such promoting vibrations have successfully reproduced and explained experimental observations, it has also been argued that such vibrations are not part of the catalytic effect. In this study, we have employed combined quantum mechanical/molecular mechanical methods with molecular dynamics and potential energy surface calculations to investigate how enzyme and substrate motion affects the energy barrier to proton transfer for the rate-limiting H-transfer step in aromatic amine dehydrogenase (AADH) with tryptamine as substrate. In particular, the conformation of the iminoquinone adduct induced by AADH was found to be essential for a promoting vibration identified previously—this lowers significantly the ‘effective’ potential energy barrier, that is the barrier which remains to be surmounted following collective, thermally equilibrated motion attaining a quantum degenerate state of reactants and products. When the substrate adopts a conformation similar to that in the free iminoquinone, this barrier was found to increase markedly. This is consistent with AADH facilitating the H-transfer event by holding the substrate in a conformation that induces a promoting vibration.

Tunneling and Classical Paths for Proton Transfer in an Enzyme Reaction Dominated by Tunneling:  Oxidation of Tryptamine by Aromatic Amine Dehydrogenase

2007 ◽  
Vol 111 (11) ◽  
pp. 3032-3047 ◽  
Author(s):  
Laura Masgrau ◽  
Kara E. Ranaghan ◽  
Nigel S. Scrutton ◽  
Adrian J. Mulholland ◽  
Michael J. Sutcliffe
Keyword(s):  
Proton Transfer ◽  
Aromatic Amine ◽  
Enzyme Reaction ◽  
Amine Dehydrogenase

Proton transfer is not rate-limiting in buffer-induced non-enzymic glucation of hemoglobin

1988 ◽  
Vol 110 (24) ◽  
pp. 8265-8266 ◽  
Author(s):  
Herminia. Gil ◽  
Julio F. Mata-Segreda ◽  
Richard L. Schowen
Keyword(s):  
Proton Transfer ◽  
Rate Limiting
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