Role of Macromolecular Crowding on Stability and Iron Release Kinetics of Serum Transferrin

2017 ◽  
Vol 121 (37) ◽  
pp. 8669-8683 ◽  
Author(s):  
Sandeep Kumar ◽  
Deepak Sharma ◽  
Rajesh Kumar
Keyword(s):  
Release Kinetics ◽  
Macromolecular Crowding ◽  
Serum Transferrin ◽  
Iron Release ◽  
Kinetics Of

scholarly journals Halloysite nanotubes filled with salicylic acid and sodium diclofenac: effects of vacuum pumping on loading and release properties

2021 ◽  
Author(s):  
Lorenzo Lisuzzo ◽  
Giuseppe Cavallaro ◽  
Stefana Milioto ◽  
Giuseppe Lazzara
Keyword(s):  
Salicylic Acid ◽  
Release Kinetics ◽  
Halloysite Nanotubes ◽  
Drug Molecules ◽  
Sodium Diclofenac ◽  
Tunable Release ◽  
Feature Based ◽  
Kinetics Of ◽  
Filling Mechanism

AbstractIn this work, we investigated the effects of the vacuum pumping on both the loading efficiencies and the release kinetics of halloysite nanotubes filled with drug molecules dissolved in ethanol. As model drugs, salicylic acid and sodium diclofenac were selected. For comparison, the loading of the drug molecules was conducted on platy kaolinite to explore the key role of the hollow tubular morphology on the filling mechanism of halloysite. The effects of the pressure conditions used in the loading protocol were interpreted and discussed on the basis of the thermodynamic results provided by Knudsen thermogravimetry, which demonstrated the ethanol confinement inside the halloysite cavity. Several techniques (TEM, FTIR spectroscopy, DLS and $$\zeta$$ ζ -potential experiments) were employed to characterize the drug filled nanoclays. Besides, release kinetics of the drugs were studied and interpreted according to the loading mechanism. This work represents a further step for the development of nanotubular carriers with tunable release feature based on the loading protocol and drug localization into the carrier. Graphic abstract The filling efficiency of halloysite nanotubes is enhanced by the reduction of the pressure conditions used in the loading protocol.

scholarly journals The Unique Kinetics of Iron Release from Transferrin: The Role of Receptor, Lobe–Lobe Interactions, and Salt at Endosomal pH

2010 ◽  
Vol 396 (1) ◽  
pp. 130-140 ◽  
Author(s):  
Shaina L. Byrne ◽  
N. Dennis Chasteen ◽  
Ashley N. Steere ◽  
Anne B. Mason
Keyword(s):  
Iron Release ◽  
Endosomal Ph ◽  
Kinetics Of

scholarly journals Endosome–mitochondria interactions are modulated by iron release from transferrin

2016 ◽  
Vol 214 (7) ◽  
pp. 831-845 ◽  
Author(s):  
Anupam Das ◽  
Sagarika Nag ◽  
Anne B. Mason ◽  
Margarida M. Barroso
Keyword(s):  
Epithelial Cells ◽  
Three Dimensional ◽  
Time Lapse ◽  
Iron Release ◽  
Iron Transfer ◽  
Distance Transformation ◽  
Stochastic Optical Reconstruction Microscopy ◽  
Optical Reconstruction ◽  
Kinetics Of

Transient “kiss and run” interactions between endosomes containing iron-bound transferrin (Tf) and mitochondria have been shown to facilitate direct iron transfer in erythroid cells. In this study, we used superresolution three-dimensional (3D) direct stochastic optical reconstruction microscopy to show that Tf-containing endosomes directly interact with mitochondria in epithelial cells. We used live-cell time-lapse fluorescence microscopy, followed by 3D rendering, object tracking, and a distance transformation algorithm, to track Tf-endosomes and characterize the dynamics of their interactions with mitochondria. Quenching of iron sensor RDA-labeled mitochondria confirmed functional iron transfer by an interacting Tf-endosome. The motility of Tf-endosomes is significantly reduced upon interaction with mitochondria. To further assess the functional role of iron in the ability of Tf-endosomes to interact with mitochondria, we blocked endosomal iron release by using a Tf K206E/K534A mutant. Blocking intraendosomal iron release led to significantly increased motility of Tf-endosomes and increased duration of endosome–mitochondria interactions. Thus, intraendosomal iron regulates the kinetics of the interactions between Tf-containing endosomes and mitochondria in epithelial cells.

scholarly journals Investigation of the Role of Cholesterol Superlattice in Release Kinetics of Drugs from Stealth Liposomes

2012 ◽  
Vol 102 (3) ◽  
pp. 83a ◽  
Author(s):  
Nicole Haloupek ◽  
Justin Rafferty ◽  
Kayla Haloupek ◽  
Emmanuel Tejada ◽  
Tamara Mamistvalova ◽  
...  
Keyword(s):  
Release Kinetics ◽  
Stealth Liposomes ◽  
Kinetics Of

The role of microsphere fabrication methods on the stability and release kinetics of ovalbumin encapsulated in polyanhydride microspheres

2006 ◽  
Vol 23 (8) ◽  
pp. 832-843 ◽  
Author(s):  
Amy S. Determan ◽  
Jennifer R. Graham ◽  
Katherine A. Pfeiffer ◽  
Balaji Narasimhan
Keyword(s):  
Release Kinetics ◽  
The Stability ◽  
Kinetics Of

scholarly journals Antibacterial Activity and Iron Release of Organic-Inorganic Hybrid Biomaterials Synthesized via the Sol-Gel Route

2021 ◽  
Vol 11 (19) ◽  
pp. 9311
Author(s):  
Michelina Catauro ◽  
Ylenia D’Errico ◽  
Antonio D’Angelo ◽  
Ronald J. Clarke ◽  
Ignazio Blanco
Keyword(s):  
Hybrid Materials ◽  
Release Kinetics ◽  
Sol Gel ◽  
Antibacterial Properties ◽  
Iron Release ◽  
Biological Characterization ◽  
Visible Spectroscopy ◽  
E Coli ◽  
Hybrid Biomaterials ◽  
Kinetics Of

The aim of this work was the synthesis of hybrid materials of iron (II)-based therapeutic systems via the sol-gel method. Increasing amounts of polyethylene glycol (PEG 6, 12, 24, 50 wt%) were added to SiO2/Fe20 wt% to modulate the release kinetics of the drug from the systems. Fourier-transform infrared (FTIR) spectroscopy was used to study the interactions between different components in the hybrid materials. The release kinetics in a simulated body fluid (SBF) were investigated, and the amount of Fe2+ released was detected via ultraviolet-visible spectroscopy (UV-Vis) after reaction with ortho-phenanthroline. Furthermore, biological characterization was carried out. The bioactivity of the synthesized hybrid materials was evaluated via the formation of a layer of hydroxyapatite on the surface of samples soaked in SBF using spectroscopy. Finally, the potential antibacterial properties of seven different materials against two different bacteria—E. coli and S. aureus—were investigated.

Investigation of the Mechanism of Iron Release from the C-Lobe of Human Serum Transferrin:  Mutational Analysis of the Role of a pH Sensitive Triad†

Biochemistry ◽  
2003 ◽  
Vol 42 (13) ◽  
pp. 3701-3707 ◽  
Author(s):  
Peter J. Halbrooks ◽  
Qing-Yu He ◽  
Sara K. Briggs ◽  
Stephen J. Everse ◽  
Valerie C. Smith ◽  
...  
Keyword(s):  
Human Serum ◽  
Mutational Analysis ◽  
Ph Sensitive ◽  
Serum Transferrin ◽  
Iron Release ◽  
Human Serum Transferrin

Electrostatic effects control the stability and iron release kinetics of ovotransferrin

2014 ◽  
Vol 19 (6) ◽  
pp. 1009-1024 ◽  
Author(s):  
Sandeep Kumar ◽  
Deepak Sharma ◽  
Rajesh Kumar ◽  
Rajesh Kumar
Keyword(s):  
Release Kinetics ◽  
Iron Release ◽  
Electrostatic Effects ◽  
The Stability ◽  
Kinetics Of
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