Thermophilic Enzyme or Mesophilic Enzyme with Enhanced Thermostability: Can We Draw a Line?

2017 ◽  
Vol 121 (29) ◽  
pp. 7086-7094 ◽  
Author(s):  
Xiaomin Jing ◽  
Wilfredo Evangelista Falcon ◽  
Jerome Baudry ◽  
Engin H. Serpersu
Keyword(s):  
Thermophilic Enzyme

scholarly journals Alicyclobacillus acidocaldarius Thermophilic Esterase EST2's Activity in Milk and Cheese Models

2006 ◽  
Vol 72 (5) ◽  
pp. 3191-3197 ◽  
Author(s):  
Luigi Mandrich ◽  
Giuseppe Manco ◽  
Mos� Rossi ◽  
Esther Floris ◽  
Tanja Jansen-van den Bosch ◽  
...  
Keyword(s):  
Short Chain Fatty Acid ◽  
Hydrolytic Activity ◽  
Spectrophotometric Assay ◽  
Fatty Acid Esters ◽  
Optimal Conditions ◽  
Ester Synthesis ◽  
Thermophilic Enzyme ◽  
Thermophilic Esterase ◽  
Alicyclobacillus Acidocaldarius ◽  
Acid Esters

ABSTRACT The aim of this work was to investigate the behavior of thermophilic esterase EST2 from Alicyclobacillus acidocaldarius in milk and cheese models. The pure enzyme was used to compare the EST2 hydrolytic activity to the activity of endogenous esterase EstA from Lactococcus lactis. The results indicate that EST2 exhibits 30-fold-higher esterase activity than EstA. As EstA has thioesterase activity, EST2 was assayed for this activity under the optimal conditions determined for EstA (namely, 30�C and pH 7.5). Although it is a thermophilic enzyme, EST2 exhibited eightfold-higher thioesterase activity than EstA with S-methyl thiobutanoate. The abilities of EST2 and EstA to synthesize short-chain fatty acid esters were compared. Two methods were developed to do this. In the first method a spectrophotometric assay was used to monitor the synthesis of esters by the pure enzymes using p-nitrophenol as the alcohol substrate. The synthetic activities were also evaluated under conditions that mimicked those present in milk and/or cheese. The second method involved evaluation of the synthetic abilities of the enzymes when they were directly added to a model cheese matrix. Substantial ester synthesis by EST2 was observed under both conditions. Finally, esterase and thioesterase activities were evaluated in milk using the purified EST2 enzyme and in the model cheese matrix using a strain of L. lactis NZ9000 harboring the EST2 gene and thus overproducing EST2. Both the esterase and thioesterase activities measured in milk and in the cheese matrix were much greater than the activities of the controls.

scholarly journals A Rigidifying Salt-Bridge Favors the Activity of Thermophilic Enzyme at High Temperatures at the Expense of Low-Temperature Activity

PLoS Biology ◽  
2011 ◽  
Vol 9 (3) ◽  
pp. e1001027 ◽  
Author(s):  
Sonia Y. Lam ◽  
Rachel C. Y. Yeung ◽  
Tsz-Ha Yu ◽  
Kong-Hung Sze ◽  
Kam-Bo Wong
Keyword(s):  
Low Temperature ◽  
High Temperatures ◽  
Salt Bridge ◽  
Thermophilic Enzyme

Biohydrogen Production by Co-Fermentation of Starch Wastewater and Sludge under Thermophilic Condition

2013 ◽  
Vol 724-725 ◽  
pp. 360-364 ◽  
Author(s):  
He Shan Zheng ◽  
Wan Qian Guo ◽  
Shan Shan Yang ◽  
Xiao Chi Feng ◽  
Xian Jiao Zhou ◽  
...  
Keyword(s):  
Low Frequency ◽  
Biohydrogen Production ◽  
Mixing Ratio ◽  
Thermophilic Enzyme ◽  
Ph Buffering ◽  
Starch Wastewater ◽  
Low Frequency Ultrasound ◽  
Thermophilic Condition ◽  
Low Intensity Ultrasound ◽  
Frequency Ultrasound

In this paper, the possibility and potential of biohydrogen production by co-fermentation of starch wastewater (SW) and WAS under thermophilic temperature was studied in batch fermentation tests. WAS was first pretreated by thermophilic enzyme together with low intensity ultrasound (LIU) to improve the biochemical degradability. Then After 8 h pretreatment, all soluble substances were much higher than raw sludge without pretreatment. In addition, the SCOD, carbohydrate and protein of the tests which were accelerated by low-frequency ultrasound were 11.5% 18.4%, 17.8% higher than the control, respectively. Results from the co-fermentation further demonstrated that the sludge had high pH buffering capacity. A mixing ratio of 1:1 was found to be the best among of all co-fermentation tests. Moreover it was proved in this study that hydrogen production by co-fermentation of starch wastewater and sludge was a promising technology to recovery energy from the waste.

Patterns of adaptation in a laboratory evolved thermophilic enzyme

2001 ◽  
Vol 1549 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Patrick L Wintrode ◽  
Kentaro Miyazaki ◽  
Frances H Arnold
Keyword(s):  
Thermophilic Enzyme

scholarly journals Concommitant adaptation of a GH11 xylanase by directed evolution to create an alkali-tolerant/thermophilic enzyme

2014 ◽  
Vol 27 (8) ◽  
pp. 255-262 ◽  
Author(s):  
Roberto Ruller ◽  
Juliana Alponti ◽  
Laila Aparecida Deliberto ◽  
Letícia Maria Zanphorlin ◽  
Carla Botelho Machado ◽  
...  
Keyword(s):  
Directed Evolution ◽  
Thermophilic Enzyme

Stabilization of an FET glucose sensor with the thermophilic enzyme glucokinase

1990 ◽  
Vol 1 (1-6) ◽  
pp. 504-507 ◽  
Author(s):  
T. Katsube ◽  
M. Katoh ◽  
H. Maekawa ◽  
M. Hara ◽  
S. Yamaguchi ◽  
...  
Keyword(s):  
Glucose Sensor ◽  
Thermophilic Enzyme
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