scholarly journals Membrane Disruption Mechanism of a Prion Peptide (106–126) Investigated by Atomic Force Microscopy, Raman and Electron Paramagnetic Resonance Spectroscopy

2017 ◽  
Vol 121 (19) ◽  
pp. 5058-5071 ◽  
Author(s):  
Jianjun Pan ◽  
Prasana K. Sahoo ◽  
Annalisa Dalzini ◽  
Zahra Hayati ◽  
Chinta M. Aryal ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Electron Paramagnetic Resonance ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Membrane Disruption ◽  
Resonance Spectroscopy ◽  
Paramagnetic Resonance ◽  
Force Microscopy ◽  
Atomic Force ◽  
Electron Paramagnetic

scholarly journals A new building block for DNA network formation by self-assembly and polymerase chain reaction

2014 ◽  
Vol 10 ◽  
pp. 1037-1046 ◽  
Author(s):  
Holger Bußkamp ◽  
Sascha Keller ◽  
Marta Robotta ◽  
Malte Drescher ◽  
Andreas Marx
Keyword(s):  
Self Assembly ◽  
Building Block ◽  
Network Formation ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Resonance Spectroscopy ◽  
Paramagnetic Resonance ◽  
Force Microscopy ◽  
Atomic Force ◽  
Polymerase Chain ◽  
Electron Paramagnetic

The predictability of DNA self-assembly is exploited in many nanotechnological approaches. Inspired by naturally existing self-assembled DNA architectures, branched DNA has been developed that allows self-assembly to predesigned architectures with dimensions on the nanometer scale. DNA is an attractive material for generation of nanostructures due to a plethora of enzymes which modify DNA with high accuracy, providing a toolbox for many different manipulations to construct nanometer scaled objects. We present a straightforward synthesis of a rigid DNA branching building block successfully used for the generation of DNA networks by self-assembly and network formation by enzymatic DNA synthesis. The Y-shaped 3-armed DNA construct, bearing 3 primer strands is accepted by Taq DNA polymerase. The enzyme uses each arm as primer strand and incorporates the branched construct into large assemblies during PCR. The networks were investigated by agarose gel electrophoresis, atomic force microscopy, dynamic light scattering, and electron paramagnetic resonance spectroscopy. The findings indicate that rather rigid DNA networks were formed. This presents a new bottom-up approach for DNA material formation and might find applications like in the generation of functional hydrogels.

scholarly journals Singlet oxygen generation by higher fullerene-based colloids

2010 ◽  
Vol 75 (7) ◽  
pp. 965-973 ◽  
Author(s):  
Svetlana Jovanovic ◽  
Zoran Markovic ◽  
Duska Kleut ◽  
Vladimir Trajkovic ◽  
Branka Babic-Stojic ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Singlet Oxygen ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Resonance Spectroscopy ◽  
Paramagnetic Resonance ◽  
Oxygen Generation ◽  
Force Microscopy ◽  
Water Based ◽  
Average Size ◽  
Electron Paramagnetic

In this paper, the results of the synthesis and characterization of higher fullerene-based colloids is presented. The generation of singlet oxygen 1O2 (1?g) by fullerene water-based colloids (nC60, nC70 and nC84) was investigated. It was found by electron paramagnetic resonance spectroscopy that the generation of singlet oxygen was the highest by the nC84 colloid. The amplitude of the electron paramagnetic resonance (EPR) signal was two orders of magnitude higher than the amplitude of the EPR signals which originated from nC60 and nC70. The surface morphology and the structure of the particles of the water-based colloids were investigated by atomic force microscopy (AFM). The AFM study showed that the average size of the nC60, nC70 and nC84 were 200 nm, 80 nm and 70 nm, respectively. In addition, the particle size distribution of the nC60, nC70 and nC84 colloids was determined by dynamic light scattering (DLS) measurements.

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