Highly Heterogeneous Nature of the Native and Unfolded States of the B Domain of Protein A Revealed by Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy

2017 ◽  
Vol 121 (22) ◽  
pp. 5463-5473 ◽  
Author(s):  
Takuhiro Otosu ◽  
Kunihiko Ishii ◽  
Hiroyuki Oikawa ◽  
Munehito Arai ◽  
Satoshi Takahashi ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Protein A ◽  
Correlation Spectroscopy ◽  
Two Dimensional ◽  
Heterogeneous Nature

scholarly journals Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy on the Folding Mechanism of B Domain of Protein A

2015 ◽  
Vol 108 (2) ◽  
pp. 501a
Author(s):  
Takuhiro Otosu ◽  
Kunihiko Ishii ◽  
Hiroyuki Oikawa ◽  
Munehito Arai ◽  
Satoshi Takahashi ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Protein A ◽  
Correlation Spectroscopy ◽  
Two Dimensional ◽  
Folding Mechanism

scholarly journals 3P052 Two-dimensional fluorescence lifetime correlation spectroscopy on the conformational dynamics of the unfolded state of BdpA(01A. Protein: Structure,Poster)

2013 ◽  
Vol 53 (supplement1-2) ◽  
pp. S220
Author(s):  
Takuhiro Otosu ◽  
Kunihiko Ishii ◽  
Hiroyuki Oikawa ◽  
Munehito Arai ◽  
Satoshi Takahashi ◽  
...  
Keyword(s):  
Protein Structure ◽  
Fluorescence Lifetime ◽  
Conformational Dynamics ◽  
Correlation Spectroscopy ◽  
Two Dimensional ◽  
Unfolded State

scholarly journals Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy: Concepts and Applications

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2972 ◽  
Author(s):  
Takuhiro Otosu ◽  
Shoichi Yamaguchi
Keyword(s):  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Fluorescence Correlation Spectroscopy ◽  
Conformational Dynamics ◽  
Correlation Spectroscopy ◽  
Two Dimensional ◽  
Single Molecule Level ◽  
Fluorescence Correlation ◽  
Promising Tool ◽  
Molecular Ruler

We review the basic concepts and recent applications of two-dimensional fluorescence lifetime correlation spectroscopy (2D FLCS), which is the extension of fluorescence correlation spectroscopy (FCS) to analyze the correlation of fluorescence lifetime in addition to fluorescence intensity. Fluorescence lifetime is sensitive to the microenvironment and can be a “molecular ruler” when combined with FRET. Utilization of fluorescence lifetime in 2D FLCS thus enables us to quantify the inhomogeneity of the system and the interconversion dynamics among different species with a higher time resolution than other single-molecule techniques. Recent applications of 2D FLCS to various biological systems demonstrate that 2D FLCS is a unique and promising tool to quantitatively analyze the microsecond conformational dynamics of macromolecules at the single-molecule level.

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