scholarly journals A Quantitative Theoretical Framework For Protein-Induced Fluorescence Enhancement–Förster-Type Resonance Energy Transfer (PIFE-FRET)

2016 ◽  
Vol 120 (26) ◽  
pp. 6401-6410 ◽  
Author(s):  
Eitan Lerner ◽  
Evelyn Ploetz ◽  
Johannes Hohlbein ◽  
Thorben Cordes ◽  
Shimon Weiss
Keyword(s):  
Energy Transfer ◽  
Fluorescence Enhancement ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Theoretical Framework

Diphenylacrylonitrile-connected BODIPY dyes: fluorescence enhancement based on dark and AIE resonance energy transfer

2017 ◽  
Vol 15 (28) ◽  
pp. 6006-6013 ◽  
Author(s):  
Liangbin Lin ◽  
Xiaoru Lin ◽  
Hongyu Guo ◽  
Fafu Yang
Keyword(s):  
Energy Transfer ◽  
Fluorescence Enhancement ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Energy Transfers ◽  
First Time

Diphenylacrylonitrile-connected BODIPY dyes were reported for the first time, and they exhibited remarkable fluorescence enhancement based on both dark and AIE resonance energy transfers.

Biotin induced fluorescence enhancement in resonance energy transfer and application for bioassay

Talanta ◽  
2009 ◽  
Vol 80 (2) ◽  
pp. 454-458 ◽  
Author(s):  
Shan Hu ◽  
Hai Yang ◽  
Ruxiu Cai ◽  
Zhenshi Liu ◽  
Xiangliang Yang
Keyword(s):  
Energy Transfer ◽  
Fluorescence Enhancement ◽  
Resonance Energy Transfer ◽  
Resonance Energy

scholarly journals Molecular-Fluorescence Enhancement via Blue-Shifted Plasmon-Induced Resonance Energy Transfer

2016 ◽  
Vol 120 (27) ◽  
pp. 14820-14827 ◽  
Author(s):  
Mingsong Wang ◽  
Bharath Bangalore Rajeeva ◽  
Leonardo Scarabelli ◽  
Evan P. Perillo ◽  
Andrew K. Dunn ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Enhancement ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Molecular Fluorescence

Combining gold nanoparticle antennas with single-molecule fluorescence resonance energy transfer (smFRET) to study DNA hairpin dynamics

Nanoscale ◽  
2018 ◽  
Vol 10 (14) ◽  
pp. 6611-6619 ◽  
Author(s):  
Jinyong Hu ◽  
Meiyan Wu ◽  
Li Jiang ◽  
Zhensheng Zhong ◽  
Zhangkai Zhou ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Gold Nanoparticle ◽  
Fluorescence Resonance Energy Transfer ◽  
Single Molecule ◽  
Fluorescence Enhancement ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Dna Hairpin ◽  
Single Molecule Fluorescence ◽  
Fluorescence Resonance

Gold nanoparticle antennas as a promising platform not only for fluorescence enhancement but also for the studies of single-molecule kinetics.

scholarly journals Fluorescence resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers

2016 ◽  
Author(s):  
Evelyn Ploetz ◽  
Eitan Lerner ◽  
Florence Husada ◽  
Martin Roelfs ◽  
SangYoon Chung ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Single Molecule ◽  
Conformational Changes ◽  
Transcription Initiation ◽  
Structural Changes ◽  
Fluorescence Enhancement ◽  
Protein Complexes ◽  
Fundamental Problem ◽  
Resonance Energy Transfer ◽  
Resonance Energy

ABSTRACTAdvanced microscopy methods allow obtaining information on (dynamic) conformational changes in biomolecules via measuring a single molecular distance in the structure. It is, however, extremely challenging to capture the full depth of a three-dimensional biochemical state, binding-related structural changes or conformational cross-talk in multi-protein complexes using one-dimensional assays. In this paper we address this fundamental problem by extending the standard molecular ruler based on Förster resonance energy transfer (FRET) into a two-dimensional assay via its combination with protein-induced fluorescence enhancement (PIFE). We show that donor brightness (viaPIFE) and energy transfer efficiency (viaFRET) can simultaneously report on e.g., the conformational state of dsDNA following its interaction with unlabelled proteins (BamHI, EcoRV, T7 DNA polymerase gp5/trx). The PIFE-FRET assay uses established labelling protocols and single molecule fluorescence detection schemes (alternating-laser excitation, ALEX). Besides quantitative studies of PIFE and FRET ruler characteristics, we outline possible applications of ALEX-based PIFE-FRET for single-molecule studies with diffusing and immobilized molecules. Finally, we study transcription initiation and scrunching ofE. coliRNA-polymerase with PIFE-FRET and provide direct evidence for the physical presence and vicinity of the polymerase that causes structural changes and scrunching of the transcriptional DNA bubble.

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