Ionic Liquid Surfactant Mediated Structural Transitions and Self-Assembly of Bovine Serum Albumin in Aqueous Media: Effect of Functionalization of Ionic Liquid Surfactants

2015 ◽  
Vol 119 (33) ◽  
pp. 10573-10585 ◽  
Author(s):  
Gurbir Singh ◽  
Tejwant Singh Kang
RSC Advances ◽  
2019 ◽  
Vol 9 (33) ◽  
pp. 19114-19120 ◽  
Author(s):  
Xingang Jia ◽  
Xiaoling Hu ◽  
Wenzhen Wang ◽  
Chunbao Du

Non-covalent binding between nanosilica and bovine serum albumin has been illustrated by experiments and theoretical calculations.


2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Sergio-Miguel Acuña-Nelson ◽  
José-Miguel Bastías-Montes ◽  
Fabiola-Rossana Cerda-Leal ◽  
Julio-Enrique Parra-Flores ◽  
Juan-Salvador Aguirre-García ◽  
...  

Protein adsorption is influenced by many factors such as temperature, pH, protein size and structure, or surface energy and roughness, among others. Self-assembled monolayers (SAMs) and the Langmuir-Blodgett (LB) technique are two of the techniques more used to produces ultrathin films of proteins on surfaces. In this work, we established protocols for the preparation of nanocoatings of bovine serum albumin (BSA) protein on glass surface using SAMs and LB. Furthermore, we determined how small changes in temperature and pH can affect the covering when SAMs are used. Using a combination of different analyses, such as relative roughness, dynamic contact angles, and atomic force microscopy (AFM), it was possible to establish conditions to obtain a uniform nanocoating using SAMs. The results of the analysis of the nanocoating performed using the LB technique were very similar to those obtained using SAMs. The Derjaguin–Landau–Verwey–Overbeek (DLVO) theory in conjunction with the AFM images showed that electrostatic interactions are very important in the self-assembly process, but a process dominated solely by attraction is not sufficient to achieve a good SAM nanocoating, since it does not allow proper orientation and packaging of BSA molecules on the glass surface.


RSC Advances ◽  
2016 ◽  
Vol 6 (24) ◽  
pp. 19700-19706 ◽  
Author(s):  
Gongke Wang ◽  
Huimin Hou ◽  
Ye Chen ◽  
Changling Yan ◽  
Guangyue Bai ◽  
...  

The interactions between decyl-β-d-glucopyranoside (DG) and bovine serum albumin (BSA), in aqueous media, were investigated through the use of surface tension, steady-state fluorescence, and UV-vis absorption spectroscopy measurements.


2015 ◽  
Vol 17 (47) ◽  
pp. 32023-32032 ◽  
Author(s):  
Nadav Amdursky

The concentration-induced structural transition of BSA at low pH was studied with photoacids. A special focus is also given to the discussion about the validity of other techniques to observe this transition.


Author(s):  
Meena Kumari ◽  
Jitendra Kumar Maurya ◽  
Upendra Kumar Singh ◽  
Abbul Bashar Khan ◽  
Maroof Ali ◽  
...  

RSC Advances ◽  
2016 ◽  
Vol 6 (95) ◽  
pp. 92349-92359
Author(s):  
Shivnetra Saha ◽  
Rupali Shekhawat ◽  
Shashank Deep

An unusual phenomenon in the aggregation profile of BSA in the presence of CTAB, brought about by stirring, is reported here.


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