From Associative to Segregative Phase Separation: The Phase Behavior of Poly(acrylate)/Dodecyltrimethylammonium Complex Salts in the Presence of NaBr and NaCl

2021 ◽  
Vol 125 (11) ◽  
pp. 2968-2975
Author(s):  
Marcos Vinícius Aquino Queirós ◽  
Watson Loh
Keyword(s):  
Phase Separation ◽  
Phase Behavior ◽  
Complex Salts ◽  
Segregative Phase Separation

Phase Behavior of Polyion−Surfactant Ion Complex Salts:  Effects of Surfactant Chain Length and Polyion Length

2006 ◽  
Vol 110 (21) ◽  
pp. 10332-10340 ◽  
Author(s):  
Anna Svensson ◽  
Jens Norrman ◽  
Lennart Piculell
Keyword(s):  
Phase Behavior ◽  
Chain Length ◽  
Complex Salts

Phase Behavior Controlled by the Addition of Long-Chain n-Alcohols in Systems of Cationic Surfactant/Anionic Polyion Complex Salts and Water

2018 ◽  
Vol 122 (18) ◽  
pp. 4861-4869 ◽  
Author(s):  
Ana M. Percebom ◽  
Guilherme A. Ferreira ◽  
Daniel Rege Catini ◽  
Juliana S. Bernardes ◽  
Watson Loh
Keyword(s):  
Phase Behavior ◽  
Cationic Surfactant ◽  
Polyion Complex ◽  
Complex Salts ◽  
Long Chain

Producing PVP Nanofibers by Electrospinning in N2

2012 ◽  
Vol 560-561 ◽  
pp. 701-708 ◽  
Author(s):  
Lu Li ◽  
Jie Xu ◽  
Tao Fang ◽  
Jin Geng ◽  
Detlef Freitag ◽  
...  
Keyword(s):  
Mass Transfer ◽  
Phase Equilibrium ◽  
Phase Separation ◽  
Phase Behavior ◽  
Polymer Solution ◽  
Aspen Plus ◽  
Good Choice ◽  
The Other ◽  
Binary Phase ◽  
Novel Method

Electrospinning combined with nonsolvent-induced phase separation is a simple and novel method to produce porous nanofibers. In the study, Poly (vinylpyrrolidone) (PVP) nanofibers were fabricated using an electrospinning approach complemented by compressed nitrogen (N). N2 was used as the nonsolvent of choice. Besides, the tun2ning of N2 pressure and temperature can impact the nanofibers’ morphologies by altering phase behavior and mass transfer. Also, the other parameters affecting electrospinning of polymer solution were discussed. The results were demonstrated by extending the technique to PVP/dichloromethane (DCM) and PVP/ethanol (EtOH) systems. And the binary phase equilibrium of solvents (dichloromethane or ethanol) and N simulated by ASPEN PLUS 2006 demonstrates that N is not a 2good choice for producing hollow or po2rous polymer nanofibers.

Phase behavior of lysozyme solutions in the liquid–liquid phase coexistence region at high hydrostatic pressures

2016 ◽  
Vol 18 (21) ◽  
pp. 14252-14256 ◽  
Author(s):  
Julian Schulze ◽  
Johannes Möller ◽  
Jonathan Weine ◽  
Karin Julius ◽  
Nico König ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Phase Behavior ◽  
High Pressures ◽  
Phase Coexistence ◽  
Protein Solutions ◽  
Separation Region ◽  
Coexistence Region ◽  
High Hydrostatic Pressures ◽  
Liquid Liquid Phase Separation

Dense protein solutions exhibit a reentrant liquid–liquid phase separation region at high pressures.

The aqueous phase behavior of polyion–surfactant ion complex salts mixed with nonionic surfactants

2011 ◽  
Vol 13 (8) ◽  
pp. 3126-3138 ◽  
Author(s):  
John Janiak ◽  
Lennart Piculell ◽  
Gerd Olofsson ◽  
Karin Schillén
Keyword(s):  
Phase Behavior ◽  
Aqueous Phase ◽  
Nonionic Surfactants ◽  
Complex Salts

scholarly journals Phase behavior of the polymer/drug system PLA/DEET: Effect of PLA molar mass on subambient liquid-liquid phase separation

2018 ◽  
Vol 660 ◽  
pp. 77-81 ◽  
Author(s):  
Chanita Sungkapreecha ◽  
Mark J. Beily ◽  
Jörg Kressler ◽  
Walter W. Focke ◽  
René Androsch
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Phase Behavior ◽  
Molar Mass ◽  
Liquid Phase Separation ◽  
Liquid Liquid Phase Separation

scholarly journals Dipeptides are minimalistic but sufficient for liquid-liquid phase separation

2021 ◽  
Author(s):  
Yiming Tang ◽  
Santu Bera ◽  
Yifei Yao ◽  
Jiyuan Zeng ◽  
Zenghui Lao ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Phase Behavior ◽  
General Property ◽  
Md Simulations ◽  
Building Blocks ◽  
Liquid Phase Separation ◽  
Sequence Length ◽  
Multivalent Interactions ◽  
Liquid Liquid Phase Separation

AbstractLiquid-liquid phase separation (LLPS) of proteins mediates the assembly of biomolecular condensates involved in physiological and pathological processes. Identifying the minimalistic building blocks and the sequence determinant of protein phase separation is of urgent importance but remains challenging due to the enormous sequence space and difficulties of existing methodologies in characterizing the phase behavior of ultrashort peptides. Here we demonstrate computational tools to efficiently quantify the microscopic fluidity and density of liquid-condensates/solid-aggregates and the temperature-dependent phase diagram of peptides. Utilizing our approaches, we comprehensively predict the LLPS abilities of all 400 dipeptide combinations of coded amino acids based on 492 micro-second molecular dynamics simulations, and observe the occurrences of spontaneous LLPS. We identify 54 dipeptides that form solid-like aggregates and three categories of dipeptides with high LLPS propensity. Our predictions are validated by turbidity assays and differential interference contrast (DIC) microscopy on four representative dipeptides (WW, QW, GF, and VI). Phase coexistence diagrams are constructed to explore the temperature dependence of LLPS. Our results reveal that aromatic moieties are crucial for a dipeptide to undergo LLPS, and hydrophobic and polar components are indispensable. We demonstrate for the first time that dipeptides, minimal but complete, possess multivalent interactions sufficient for LLPS, suggesting that LLPS is a general property of peptides/proteins, independent of their sequence length. This study provides a computational and experimental approach to the prediction and characterization of the phase behavior of minimalistic peptides, and will be helpful for understanding the sequence-dependence and molecular mechanism of protein phase separation.SignificanceProtein liquid-liquid phase separation (LLPS) is associated with human health and diseases. Identifying the minimalistic building blocks and sequence determinants of LLPS is of urgent importance but remains computationally challenging partially due to the lack of methodologies characterizing the liquid condensates. Herein we provide approaches to evaluate LLPS ability of dipeptides, and screen all 400 dipeptides by MD simulations combined with multi-bead-per-residue models which capture key interactions driving LLPS that are missing in one-bead-per-residue models. Three categories of LLPS dipeptides are identified and the experimentally-verified QW dipeptide is by far the smallest LLPS system. Our results suggest that dipeptides, minimal but complete, possess multivalent interactions sufficient for LLPS, and LLPS is a general property of peptides/proteins, independent of their length.

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