Comparative Femtosecond Spectroscopy of Primary Photoreactions of Exiguobacterium sibiricum Rhodopsin and Halobacterium salinarum Bacteriorhodopsin

2021 ◽  
Vol 125 (4) ◽  
pp. 995-1008
Author(s):  
Olga A. Smitienko ◽  
Tatiana B. Feldman ◽  
Lada E. Petrovskaya ◽  
Oksana V. Nekrasova ◽  
Marina A. Yakovleva ◽  
...  
Keyword(s):  
Femtosecond Spectroscopy ◽  
Halobacterium Salinarum

scholarly journals Enhanced hydrogen production of Rhodobacter sphaeroides promoted by extracellular H+ of Halobacterium salinarum

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Jiang-Yu Ye ◽  
Yue Pan ◽  
Yong Wang ◽  
Yi-Chao Wang
Keyword(s):  
Hydrogen Production ◽  
Production Rate ◽  
Rhodobacter Sphaeroides ◽  
Coupled System ◽  
Purple Membrane ◽  
Halobacterium Salinarum ◽  
Control Group ◽  
Hydrogen Production Rate ◽  
Production Of Hydrogen ◽  
The Impact

Abstract Purpose This study utilized the principle that the bacteriorhodopsin (BR) produced by Halobacterium salinarum could increase the hydrogen production of Rhodobacter sphaeroides. H. salinarum are co-cultured with R. sphaeroides to determine the impact of purple membrane fragments (PM) on R. sphaeroides and improve its hydrogen production capacity. Methods In this study, low-salinity in 14 % NaCl domesticates H salinarum. Then, 0–160 nmol of different concentration gradient groups of bacteriorhodopsin (BR) and R. sphaeroides was co-cultivated, and the hydrogen production and pH are measured; then, R. sphaeroides and immobilized BR of different concentrations are used to produce hydrogen to detect the amount of hydrogen. Two-chamber microbial hydrogen production system with proton exchange membrane-assisted proton flow was established, and the system was operated. As additional electricity added under 0.3 V, the hydrogen production rate increased with voltages in the coupled system. Results H salinarum can still grow well after low salt in 14% NaCl domestication. When the BR concentration is 80 nmol, the highest hydrogen production reached 217 mL per hour. Both immobilized PC (packed cells) and immobilized PM (purple membrane) of H. salinarum could promote hydrogen production of R. sphaeroides to some extent. The highest production of hydrogen was obtained by the coupled system with 40 nmol BR of immobilized PC, which increased from 127 to 232 mL, and the maximum H2 production rate was 18.2 mL−1 h−1 L culture. In the 192 h experiment time, when the potential is 0.3 V, the hydrogen production amount can reach 920 mL, which is 50.3% higher than the control group. Conclusions The stability of the system greatly improved after PC was immobilized, and the time for hydrogen production of R. sphaeroides significantly extended on same condition. As additional electricity added under 0.3 V, the hydrogen production rate increased with voltages in the coupled system. These results are helpful to build a hydrogen production-coupled system by nitrogenase of R. sphaeroides and proton pump of H. salinarum. Graphical abstract

Ultrafast Dynamics of BiFeO3 Thin Films Studied by Dual-Color Femtosecond Spectroscopy

2010 ◽  
Vol 24 (1-2) ◽  
pp. 731-734 ◽  
Author(s):  
L. Y. Chen ◽  
J. C. Yang ◽  
H. C. Shih ◽  
C. W. Liang ◽  
T.-Y. Wu ◽  
...  
Keyword(s):  
Thin Films ◽  
Femtosecond Spectroscopy ◽  
Ultrafast Dynamics ◽  
Bifeo3 Thin Films ◽  
Dual Color

Femtosecond spectroscopy in amorphous silicon and silicon-germanium alloys

1987 ◽  
Vol 97-98 ◽  
pp. 145-148 ◽  
Author(s):  
P.M. Fauchet ◽  
D. Hulin ◽  
A. Migus ◽  
A. Antonetti ◽  
J.P. Conde ◽  
...  
Keyword(s):  
Amorphous Silicon ◽  
Silicon Germanium ◽  
Femtosecond Spectroscopy ◽  
Silicon Germanium Alloys

Femtosecond spectroscopy reveals huge differences in the photoisomerisation dynamics between azobenzenes linked to polymers and azobenzenes in solution

2014 ◽  
Vol 16 (23) ◽  
pp. 11549 ◽  
Author(s):  
Julia Bahrenburg ◽  
Falk Renth ◽  
Friedrich Temps ◽  
Felix Plamper ◽  
Walter Richtering
Keyword(s):  
Femtosecond Spectroscopy

scholarly journals Systems Analysis of Bioenergetics and Growth of the Extreme Halophile Halobacterium salinarum

2009 ◽  
Vol 5 (4) ◽  
pp. e1000332 ◽  
Author(s):  
Orland Gonzalez ◽  
Susanne Gronau ◽  
Friedhelm Pfeiffer ◽  
Eduardo Mendoza ◽  
Ralf Zimmer ◽  
...  
Keyword(s):  
Systems Analysis ◽  
Halobacterium Salinarum ◽  
Extreme Halophile
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