Characterizing the Brownian Diffusion of Nanocolloids and Molecular Solutions: Diffusion-Ordered NMR Spectroscopy vs Dynamic Light Scattering

2020 ◽  
Vol 124 (22) ◽  
pp. 4631-4650 ◽  
Author(s):  
Chengqi Zhang ◽  
Zhicheng Jin ◽  
Birong Zeng ◽  
Wentao Wang ◽  
Goutam Palui ◽  
...  
Keyword(s):  
Light Scattering ◽  
Nmr Spectroscopy ◽  
Dynamic Light Scattering ◽  
Brownian Diffusion

scholarly journals AHSP (α-haemoglobin-stabilizing protein) stabilizes apo-α-haemoglobin in a partially folded state

2010 ◽  
Vol 432 (2) ◽  
pp. 275-282 ◽  
Author(s):  
Kaavya Krishna Kumar ◽  
Claire F. Dickson ◽  
Mitchell J. Weiss ◽  
Joel P. Mackay ◽  
David A. Gell
Keyword(s):  
Light Scattering ◽  
Nmr Spectroscopy ◽  
Dynamic Light Scattering ◽  
Redox Activity ◽  
Heterodimeric Complex ◽  
Self Association ◽  
Partially Folded State ◽  
Haem Iron

To produce functional Hb (haemoglobin), nascent α-globin (αo) and β-globin (βo) chains must each bind a single haem molecule (to form αh and βh) and interact together to form heterodimers. The precise sequence of binding events is unknown, and it has been suggested that additional factors might enhance the efficiency of Hb folding. AHSP (α-haemoglobin-stabilizing protein) has been shown previously to bind αh and regulate redox activity of the haem iron. In the present study, we used a combination of classical and dynamic light scattering and NMR spectroscopy to demonstrate that AHSP forms a heterodimeric complex with αo that inhibits αo aggregation and promotes αo folding in the absence of haem. These findings indicate that AHSP may function as an αo-specific chaperone, and suggest an important role for αo in guiding Hb assembly by stabilizing βo and inhibiting off-pathway self-association of βh.

Size and shape trump charge in interactions of oxovanadates with self-assembled interfaces: application of continuous shape measure analysis to the decavanadate anion

2016 ◽  
Vol 40 (2) ◽  
pp. 962-975 ◽  
Author(s):  
Irma Sánchez-Lombardo ◽  
Bharat Baruah ◽  
Santiago Alvarez ◽  
Katarina R. Werst ◽  
Nicole A. Segaline ◽  
...  
Keyword(s):  
Light Scattering ◽  
Nmr Spectroscopy ◽  
Dynamic Light Scattering ◽  
Shape Analysis ◽  
Reverse Micelles ◽  
Size And Shape ◽  
Measure Analysis ◽  
Self Assembled

Using 51V NMR spectroscopy, dynamic light scattering and continuous shape analysis to characterize two polyoxometalate-encapsulation in reverse micelles.

Dynamic light scattering and 31P NMR spectroscopy study of the self-assembly of deoxyguanosine 5'-monophosphate

2002 ◽  
Vol 7 (1) ◽  
pp. 95-102 ◽  
Author(s):  
L. Spindler ◽  
I. Drevenšek Olenik ◽  
M. Čopič ◽  
R. Romih ◽  
J. Cerar ◽  
...  
Keyword(s):  
Light Scattering ◽  
Nmr Spectroscopy ◽  
Dynamic Light Scattering ◽  
Self Assembly ◽  
31P Nmr ◽  
The Self ◽  
31P Nmr Spectroscopy ◽  
Spectroscopy Study

Aggregation of aqueous lysozyme solutions followed by dynamic light scattering and 1H NMR spectroscopy

2005 ◽  
Vol 121 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Jarosław Poznański ◽  
Jędrzej Szymaǹski ◽  
Teresa Basiǹska ◽  
Stanisław Słomkowski ◽  
Wojciech Zielenkiewicz
Keyword(s):  
Light Scattering ◽  
Nmr Spectroscopy ◽  
Dynamic Light Scattering ◽  
1H Nmr ◽  
1H Nmr Spectroscopy

Characterization of Polyglycerol Polyricinoleate Formulations Using NMR Spectroscopy, Mass Spectrometry and Dynamic Light Scattering

2012 ◽  
Vol 90 (1) ◽  
pp. 39-51 ◽  
Author(s):  
Andreas Orfanakis ◽  
Emmanuel Hatzakis ◽  
Katerina Kanaki ◽  
Spiros A. Pergantis ◽  
Apostolos Rizos ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Light Scattering ◽  
Nmr Spectroscopy ◽  
Dynamic Light Scattering ◽  
Polyglycerol Polyricinoleate

scholarly journals Synthesis of polyampholytic diblock copolymers via RAFT aqueous solution polymerization

2021 ◽  
Author(s):  
S. M. North ◽  
S. P. Armes
Keyword(s):  
Aqueous Solution ◽  
Light Scattering ◽  
Nmr Spectroscopy ◽  
Dynamic Light Scattering ◽  
Diblock Copolymers ◽  
Solution Polymerization ◽  
H Nmr ◽  
Aqueous Solution Polymerization ◽  
Solution Behaviour

Two new classes of polyampholytic diblock copolymers are prepared via RAFT aqueous solution polymerization and their aqueous solution behaviour is assessed using 1H NMR spectroscopy, dynamic light scattering and aqueous electrophoresis measurements.

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