scholarly journals Facile Background Discrimination in Femtosecond Stimulated Raman Spectroscopy Using a Dual-Frequency Raman Pump Technique

2019 ◽  
Vol 123 (37) ◽  
pp. 7932-7939 ◽  
Author(s):  
Kajari Bera ◽  
Siu Yi Kwang ◽  
Alyssa A. Cassabaum ◽  
Christopher C. Rich ◽  
Renee R. Frontiera
Keyword(s):  
Raman Spectroscopy ◽  
Dual Frequency ◽  
Raman Pump ◽  
Stimulated Raman

scholarly journals Improved spectral resolution of the femtosecond stimulated Raman spectroscopy achieved by the use of the 2nd-order diffraction method

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dong-gu Kang ◽  
Kyung Chul Woo ◽  
Do Hyung Kang ◽  
Chanho Park ◽  
Sang Kyu Kim
Keyword(s):  
Raman Spectroscopy ◽  
Spectral Resolution ◽  
Diffraction Method ◽  
High Spectral Resolution ◽  
Double Pass ◽  
Time Resolved ◽  
Order Diffraction ◽  
Pump Laser Pulse ◽  
Raman Pump ◽  
Stimulated Raman

AbstractProlongation of the picosecond Raman pump laser pulse in the femtosecond stimulated Raman spectroscopy (FSRS) setup is essential for achieving the high spectral resolution of the time-resolved vibrational Raman spectra. In this work, the 2nd-order diffraction has been firstly employed in the double-pass grating filter technique for realizing the FSRS setup with the sub-5 cm−1 spectral resolution. It has been experimentally demonstrated that our new FSRS setup gives rise to a highly-resolved Raman spectrum of the excited trans-stilbene, which is much improved from those reported in the literatures. The spectral resolution of the present FSRS system has been estimated to be the lowest value ever reported to date, giving Δν = 2.5 cm−1.

scholarly journals Excited State Structural Evolution of a GFP Single-Site Mutant Tracked by Tunable Femtosecond-Stimulated Raman Spectroscopy

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2226 ◽  
Author(s):  
Longteng Tang ◽  
Liangdong Zhu ◽  
Miles Taylor ◽  
Yanli Wang ◽  
S. Remington ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Excited State ◽  
Time Constant ◽  
Fluorescent Protein ◽  
Stable State ◽  
Structural Evolution ◽  
Single Site ◽  
Vibrational Cooling ◽  
Raman Pump ◽  
Stimulated Raman

Tracking vibrational motions during a photochemical or photophysical process has gained momentum, due to its sensitivity to the progression of reaction and change of environment. In this work, we implemented an advanced ultrafast vibrational technique, femtosecond-stimulated Raman spectroscopy (FSRS), to monitor the excited state structural evolution of an engineered green fluorescent protein (GFP) single-site mutant S205V. This mutation alters the original excited state proton transfer (ESPT) chain. By strategically tuning the Raman pump to different wavelengths (i.e., 801, 539, and 504 nm) to achieve pre-resonance with transient excited state electronic bands, the characteristic Raman modes of the excited protonated (A*) chromophore species and intermediate deprotonated (I*) species can be selectively monitored. The inhomogeneous distribution/population of A* species go through ESPT with a similar ~300 ps time constant, confirming that bridging a water molecule to protein residue T203 in the ESPT chain is the rate-limiting step. Some A* species undergo vibrational cooling through high-frequency motions on the ~190 ps time scale. At early times, a portion of the largely protonated A* species could also undergo vibrational cooling or return to the ground state with a ~80 ps time constant. On the photoproduct side, a ~1330 cm−1 delocalized motion is observed, with dispersive line shapes in both the Stokes and anti-Stokes FSRS with a pre-resonance Raman pump, which indicates strong vibronic coupling, as the mode could facilitate the I* species to reach a relatively stable state (e.g., the main fluorescent state) after conversion from A*. Our findings disentangle the contributions of various vibrational motions active during the ESPT reaction, and offer new structural dynamics insights into the fluorescence mechanisms of engineered GFPs and other analogous autofluorescent proteins.

A POWERFUL TOOL TO STUDY COLLISIONAL PHENOMENA AND FOR COMBUSTION APPLICATIONS : THE HIGH RESOLUTION STIMULATED RAMAN SPECTROSCOPY

1987 ◽  
Vol 48 (C7) ◽  
pp. C7-761-C7-762
Author(s):  
B. LAVOREL ◽  
G. MILLOT ◽  
R. SAINT-LOUP ◽  
M. L. GONZE ◽  
J. SANTOS ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
High Resolution ◽  
Stimulated Raman

Stimulated Raman spectroscopy with 0π pulses

2014 ◽  
Vol 39 (4) ◽  
pp. 766 ◽  
Author(s):  
Zoe-Elizabeth Sariyanni ◽  
Dong Sun ◽  
Yuri V. Rostovtsev
Keyword(s):  
Raman Spectroscopy ◽  
Stimulated Raman

Identification of the Excited-State C═C and C═O Modes of trans-β-Apo-8′-carotenal with Transient 2D-IR-EXSY and Femtosecond Stimulated Raman Spectroscopy

2015 ◽  
Vol 6 (9) ◽  
pp. 1592-1598 ◽  
Author(s):  
Mariangela Di Donato ◽  
Elena Ragnoni ◽  
Andrea Lapini ◽  
Tomasz M. Kardaś ◽  
Boźena Ratajska-Gadomska ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Excited State ◽  
2D Ir ◽  
Stimulated Raman
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