Investigation on the Behavior of Noise in Asynchronous Spectra in Generalized Two-Dimensional (2D) Correlation Spectroscopy and Application of Butterworth Filter in the Improvement of Signal-to-Noise Ratio of 2D Asynchronous Spectra

2017 ◽  
Vol 121 (40) ◽  
pp. 7524-7533 ◽  
Author(s):  
Anqi He ◽  
Xianzhe Zeng ◽  
Yizhuang Xu ◽  
Isao Noda ◽  
Yukihiro Ozaki ◽  
...  
2017 ◽  
Vol 56 (25) ◽  
pp. 7188 ◽  
Author(s):  
Mingbo Chi ◽  
Yihui Wu ◽  
Fang Qian ◽  
Peng Hao ◽  
Wenchao Zhou ◽  
...  

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Elena Cerutti ◽  
Morgana D’Amico ◽  
Isotta Cainero ◽  
Gaetano Ivan Dellino ◽  
Mario Faretta ◽  
...  

AbstractQuantifying the imaging performances in an unbiased way is of outmost importance in super-resolution microscopy. Here, we describe an algorithm based on image correlation spectroscopy (ICS) that can be used to assess the quality of super-resolution images. The algorithm is based on the calculation of an autocorrelation function and provides three different parameters: the width of the autocorrelation function, related to the spatial resolution; the brightness, related to the image contrast; the relative noise variance, related to the signal-to-noise ratio of the image. We use this algorithm to evaluate the quality of stimulated emission depletion (STED) images of DNA replication foci in U937 cells acquired under different imaging conditions. Increasing the STED depletion power improves the resolution but may reduce the image contrast. Increasing the number of line averages improves the signal-to-noise ratio but facilitates the onset of photobleaching and subsequent reduction of the image contrast. Finally, we evaluate the performances of two different separation of photons by lifetime tuning (SPLIT) approaches: the method of tunable STED depletion power and the commercially available Leica Tau-STED. We find that SPLIT provides an efficient way to improve the resolution and contrast in STED microscopy.


Author(s):  
E. N. Velichko ◽  
O. I. Kotov ◽  
E. K. Nepomnyashchaya ◽  
A. N. Petrov ◽  
A. V. Sokolov

Introduction. Laser correlation spectroscopy is a promising method that allows one to analyze sizes of nanoparticles and to evaluate their shape and dynamics of aggregation in liquids. A limited usage of laser correlation spectroscopy is currently caused by insufficient accuracy of existing instruments and data processing algorithms. The paper described the development of laser correlation spectroscopic hardware complex designed for nanoparticles size determination in liquids. The basic requirements for the elements of the device and the approaches used to calculate the signal-to-noise ratio were discussed. The achieved parameters of the laser correlation spectrometer were presented.Aim. To develop the hardware for nanoparticles size determination in liquids and to optimize the parameters of hardware elements to increase signal-to-noise ratio.Materials and methods. Theory of dynamic light scattering to describe scattering of laser radiation in liquids was applied. Fundamental requirements for the elements of the laser correlation spectrometer were described.Results. An original scheme of the laser correlation spectrometer was developed, the basic requirements for the general scheme elements were described. Equations for calculating signal-to-noise ratio were given.Conclusion. The analysis of the main parameters of the elements of the laser correlation spectroscopic scheme were carried out. It helps one to evaluate the expected signal-to-noise ratio in laser correlation spectrometers.


2009 ◽  
Vol 81 (2) ◽  
pp. 834-839 ◽  
Author(s):  
Jérôme Wenger ◽  
Davy Gérard ◽  
Heykel Aouani ◽  
Hervé Rigneault ◽  
Bryan Lowder ◽  
...  

2021 ◽  
Vol 136 (1) ◽  
Author(s):  
F. Riggi ◽  
M. Bandieramonte ◽  
U. Becciani ◽  
D. L. Bonanno ◽  
G. Bonanno ◽  
...  

AbstractExperimental results from a dataset collected with a full-scale muon tomograph for the inspection of cargo containers were studied in a single scattering scenario with a multiparametric analysis based on the method of the Point Of Closest Approach (Poca). To search for high-Z materials, a 4 $$\hbox {dm}^3$$ dm 3 Pb block was positioned inside the volume to be inspected, in order to quantitatively investigate the appearance of the Poca signal. Signal-to-noise ratio and significance of the Poca signal were investigated by means of mono-dimensional spectra of the Poca components, for different values of the scattering angle between the incoming and outgoing muon tracks and with different angle-dependent weights. A systematic scan of two-dimensional maps was also carried out, as a strategy to search for possible enhancements to the Poca signal. A comparison was also done between the results obtained from the two half-volumes, one containing the Pb block and one left empty, to take into account the response of the detector and some aspects of the Poca strategy.


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