Synthesis of Water-Soluble Thioglycosylated trans-A2B2 Type Porphyrins: Cellular Uptake Studies and Photodynamic Efficiency

2020 ◽  
Vol 85 (10) ◽  
pp. 6309-6322 ◽  
Author(s):  
Vijayalakshmi Pandey ◽  
Md Kausar Raza ◽  
Pooja Joshi ◽  
Iti Gupta
Keyword(s):  
Cellular Uptake ◽  
Water Soluble ◽  
Uptake Studies

scholarly journals Epigallocatechin Gallate-Gold Nanoparticles Exhibit Superior Antitumor Activity Compared to Conventional Gold Nanoparticles: Potential Synergistic Interactions

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 396 ◽  
Author(s):  
Suhash Chavva ◽  
Sachin Deshmukh ◽  
Rajashekhar Kanchanapally ◽  
Nikhil Tyagi ◽  
Jason Coym ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Antitumor Activity ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Nuclear Translocation ◽  
Epigallocatechin Gallate ◽  
Drug Carriers ◽  
Water Soluble ◽  
Tetrazolium Salt ◽  
Uptake Studies

Epigallocatechin gallate (EGCG) possesses significant antitumor activity and binds to laminin receptors, overexpressed on cancer cells, with high affinity. Gold nanoparticles (GNPs) serve as excellent drug carriers and protect the conjugated drug from enzymatic metabolization. Citrate-gold nanoparticles (C-GNPs) and EGCG-gold nanoparticles (E-GNPs) were synthesized by reduction methods and characterized with UV-visible spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering (DLS). Cytotoxicity of citrate, EGCG, C-GNPs, and E-GNPs was evaluated by the water-soluble tetrazolium salt (WST-1) assay. Nanoparticle cellular uptake studies were performed by TEM and atomic absorption spectroscopy (AAS). Dialysis method was employed to assess drug release. Cell viability studies showed greater growth inhibition by E-GNPs compared to EGCG or C-GNPs. Cellular uptake studies revealed that, unlike C-GNPs, E-GNPs were taken up more efficiently by cancerous cells than noncancerous cells. We found that E-GNP nanoformulation releases EGCG in a sustained fashion. Furthermore, data showed that E-GNPs induced more apoptosis in cancer cells compared to EGCG and C-GNPs. From the mechanistic standpoint, we observed that E-GNPs inhibited the nuclear translocation and transcriptional activity of nuclear factor-kappaB (NF-κB) with greater potency than EGCG, whereas C-GNPs were only minimally effective. Altogether, our data suggest that E-GNPs can serve as potent tumor-selective chemotoxic agents.

scholarly journals Nanostructured Lipid Carriers as Promising Delivery Systems for Plant Extracts: The Case of Silymarin

2018 ◽  
Vol 8 (7) ◽  
pp. 1163 ◽  
Author(s):  
Vieri Piazzini ◽  
Beatrice Lemmi ◽  
Mario D’Ambrosio ◽  
Lorenzo Cinci ◽  
Cristina Luceri ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Physical Stability ◽  
In Vitro Release ◽  
Nanostructured Lipid Carriers ◽  
Water Soluble ◽  
Fluorescent Nanoparticles ◽  
Uptake Mechanism ◽  
Water Soluble Drug ◽  
Uptake Studies

Background: Silymarin is the extract from seeds of Silybum marianum L. Gaertn. and it has been used for decades as hepatoprotectant. Recently, it has been proposed to be beneficial in type 2 diabetes patients. However, silymarin is a poorly water soluble drug with limited oral bioavailability. In this study, nanostructured lipid carriers were proposed to enhance its solubility and intestinal absorption. Methods: Nanostructured lipid carriers were made of Stearic acid:Capryol 90 as lipid mixtures and Brij S20 as surfactant. Formulations were physically and chemically characterized. Stability and in vitro release studies were also assessed. In vitro permeability and Caco-2 cellular uptake mechanism were investigated. Results: Obtained results were based on size, homogeneity, ζ-potential and EE%. Nanostructured lipid carriers could be orally administered. No degradation phenomena were observed in simulated gastrointestinal fluids. Storage stability of suspensions and lyophilized products was also tested. Glucose was selected as best cryoprotectant agent. About 60% of silymarin was released in 24 h in phosphate buffered saline. In vitro parallel artificial membrane permeability assay experiments revealed that the nanocarrier enhanced the permeation of Silymarin. Caco-2 study performed with fluorescent nanoparticles revealed the ability of carrier to enhance the permeation of a lipophilic probe. Cellular uptake studies indicated that active process is involved in the internalization of the formulation. Conclusions: The optimized nanostructured lipid carriers showed excellent chemical and physical stability and enhanced the absorption of silymarin.

Water-soluble amphiphilic ruthenium(ii) polypyridyl complexes as potential light-activated therapeutic agents

2020 ◽  
Vol 56 (65) ◽  
pp. 9332-9335
Author(s):  
Sandra Estalayo-Adrián ◽  
Salvador Blasco ◽  
Sandra A. Bright ◽  
Gavin J. McManus ◽  
Guillermo Orellana ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Therapeutic Agents ◽  
Water Soluble ◽  
Polypyridyl Complexes ◽  
Cervical Cancer Cells

Two new water-soluble amphiphilic Ru(ii) polypyridyl complexes were synthesised and their photophysical and photobiological properties evaluated; both complexes showed a rapid cellular uptake and phototoxicity against HeLa cervical cancer cells.

scholarly journals In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1906
Author(s):  
Mona Atabakhshi-Kashi ◽  
Mónica Carril ◽  
Hossein Mahdavi ◽  
Wolfgang J. Parak ◽  
Carolina Carrillo-Carrion ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cellular Uptake ◽  
Hydrophobic Interactions ◽  
Intrinsic Properties ◽  
Wide Range ◽  
Self Assembled ◽  
Uptake Studies ◽  
Targeting Ability ◽  
Fluorinated Nanoparticles

Nanoparticles (NPs) functionalized with antibodies (Abs) on their surface are used in a wide range of bioapplications. Whereas the attachment of antibodies to single NPs to trigger the internalization in cells via receptor-mediated endocytosis has been widely studied, the conjugation of antibodies to larger NP assemblies has been much less explored. Taking into account that NP assemblies may be advantageous for some specific applications, the possibility of incorporating targeting ligands is quite important. Herein, we performed the effective conjugation of antibodies onto a fluorescent NP assembly, which consisted of fluorinated Quantum Dots (QD) self-assembled through fluorine–fluorine hydrophobic interactions. Cellular uptake studies by confocal microscopy and flow cytometry revealed that the NP assembly underwent the same uptake procedure as individual NPs; that is, the antibodies retained their targeting ability once attached to the nanoassembly, and the NP assembly preserved its intrinsic properties (i.e., fluorescence in the case of QD nanoassembly).

scholarly journals EFFECTS OF POLYMERIC NANOPARTICLE SURFACE PROPERTIES ON INTERACTION WITH BRAIN TUMOR ENVIRONMENT

Nano LIFE ◽  
2013 ◽  
Vol 03 (04) ◽  
pp. 1343003 ◽  
Author(s):  
BRANDON MATTIX ◽  
THOMAS MOORE ◽  
OLGA UVAROV ◽  
SAMUEL POLLARD ◽  
LAUREN O'DONNELL ◽  
...  
Keyword(s):  
Human Brain ◽  
Surface Charge ◽  
Cellular Uptake ◽  
Drug Clearance ◽  
Cellular Interaction ◽  
Normal Tissues ◽  
Poly Ethylene ◽  
Uptake Studies ◽  
Effect Of Surface

Current chemotherapy treatments are limited by poor drug solubility, rapid drug clearance and systemic side effects. Additionally, drug penetration into solid tumors is limited by physical diffusion barriers [e.g., extracellular matrix (ECM)]. Nanoparticle (NP) blood circulation half-life, biodistribution and ability to cross extracellular and cellular barriers will be dictated by NP composition, size, shape and surface functionality. Here, we investigated the effect of surface charge of poly(lactide)-poly(ethylene glycol) NPs on mediating cellular interaction. Polymeric NPs of equal sizes were used that had two different surface functionalities: negatively charged carboxyl ( COOH ) and neutral charged methoxy ( OCH 3). Cellular uptake studies showed significantly higher uptake in human brain cancer cells compared to noncancerous human brain cells, and negatively charged COOH NPs were uptaken more than neutral OCH 3 NPs in 2D culture. NPs were also able to load and control the release of paclitaxel (PTX) over 19 days. Toxicity studies in U-87 glioblastoma cells showed that PTX-loaded NPs were effective drug delivery vehicles. Effect of surface charge on NP interaction with the ECM was investigated using collagen in a 3D cellular uptake model, as collagen content varies with the type of cancer and the stage of the disease compared to normal tissues. Results demonstrated that NPs can effectively diffuse across an ECM barrier and into cells, but NP mobility is dictated by surface charge. In vivo biodistribution of OCH 3 NPs in intracranial tumor xenografts showed that NPs more easily accumulated in tumors with less collagen. These results indicate that a robust understanding of NP interaction with various tumor environments can lead to more effective patient-tailored therapies.

scholarly journals Nano-Bio Interaction between Blood Plasma Proteins and Water-Soluble Silicon Quantum Dots with Enabled Cellular Uptake and Minimal Cytotoxicity

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2250
Author(s):  
Shanmugavel Chinnathambi ◽  
Nobutaka Hanagata ◽  
Tomohiko Yamazaki ◽  
Naoto Shirahata
Keyword(s):  
Quantum Dots ◽  
Blood Plasma ◽  
Cellular Uptake ◽  
Plasma Proteins ◽  
Protein Corona ◽  
Water Soluble ◽  
Pluronic F127 ◽  
Infrared Light ◽  
Spectral Window ◽  
Blood Plasma Proteins

A better understanding of the compatibility of water-soluble semiconductor quantum dots (QDs) upon contact with the bloodstream is important for biological applications, including biomarkers working in the first therapeutic spectral window for deep tissue imaging. Herein, we investigated the conformational changes of blood plasma proteins during the interaction with near-infrared light-emitting nanoparticles, consisting of Pluronic F127 shells and cores comprised of assembled silicon QDs terminated with decane monolayers. Albumin and transferrin have high quenching constants and form a hard protein corona on the nanoparticle. In contrast, fibrinogen has low quenching constants and forms a soft protein corona. A circular dichroism (CD) spectrometric study investigates changes in the protein’s secondary and tertiary structures with incremental changes in the nanoparticle concentrations. As expected, the addition of nanoparticles causes the denaturation of the plasma proteins. However, it is noteworthy that the conformational recovery phenomena are observed for fibrinogen and transferrin, suggesting that the nanoparticle does not influence the ordered structure of proteins in the bloodstream. In addition, we observed enabled cellular uptake (NIH3T3 Fibroblasts) and minimal cytotoxicity using different cell lines (HeLa, A549, and NIH3T3). This study offers a basis to design QDs without altering the biomacromolecule’s original conformation with enabled cellular uptake with minimal cytotoxicity.

Uptake and Conversion of Selenium by a Marine Bacterium

1983 ◽  
Vol 40 (S2) ◽  
pp. s215-s220 ◽  
Author(s):  
A. Foda ◽  
J. H. Vandermeulen ◽  
J. J. Wrench
Keyword(s):  
Amino Acid ◽  
Marine Bacterium ◽  
Lipid Fraction ◽  
Water Soluble ◽  
Gas Liquid Chromatography ◽  
Bacterial Cells ◽  
Marine Broth ◽  
Simultaneous Measurements ◽  
Uptake Studies ◽  
Bacterial Lipid

Bio-conversion of Se was examined by incubating Pseudomonas marina in seawater containing either selenite (Na2SeIVO3) or selenate (Na2SeVIO4). At the concentrations of selenite and selenate used (10−4–10−7 mol/L), the growth of P. marina was not inhibited. Under these conditions, selenite was taken up by P. marina, but selenate was not found to enter the cells. Pseudomonas marina incorporated selenite from filtered seawater into sub-cellular fractions, primarily protein (30–50%) and amino acids (44–70%). When incubated in marine broth, P. marina incorporated 75selenite primarily into protein (up to 75%), with a lesser amount into the amino acid fraction (approximately 25%). Insignificant amounts were associated with the bacterial lipid fraction. SeIV was found in the protein and amino acid fractions within 10 min of incubation in medium containing selenite. In uptake studies the level of SeIV in the incubating medium decreased markedly, corresponding presumably to SeIV entering the bacterial cells from the medium. However, simultaneous measurements of total Se in the medium (gas–liquid chromatography following photooxidation) revealed an increasing amount of non-SeIV species of Se in the medium throughout the same period. These results were interpreted as due to the bioconversion of selenite by P. marina into water-soluble non-SeIV metabolite(s) and their subsequent release back into the medium. Up to 35% of the total Se found in the medium after 24 h can be accounted for by this conversion of SeIV into another oxidation state. Pseudomonas marina is also capable of reducing SeIV to elemental Se; this pathway becomes increasingly evident at higher concentrations of Na2SeO3.

A Novel Flow-Cytometry-Based Assay for Cellular Uptake Studies of Polyelectrolyte Microcapsules

Small ◽  
2008 ◽  
Vol 4 (10) ◽  
pp. 1763-1768 ◽  
Author(s):  
Maximilian Semmling ◽  
Oliver Kreft ◽  
Almudena Muñoz Javier ◽  
Gleb B. Sukhorukov ◽  
Josef Käs ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cellular Uptake ◽  
Polyelectrolyte Microcapsules ◽  
Uptake Studies

Biological activities and cellular uptake studies of fluorescent derivatives of indole alkaloid tumor promoter teleocidin

1989 ◽  
Vol 43 (3) ◽  
pp. 513-519 ◽  
Author(s):  
Kazuhiro Irie ◽  
Shigenori Okuno ◽  
Koichi Koshimizu ◽  
Harukuni Tokuda ◽  
Hoyoku Nishino ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Biological Activities ◽  
Indole Alkaloid ◽  
Tumor Promoter ◽  
Uptake Studies ◽  
Derivatives Of ◽  
Fluorescent Derivatives
Sign in / Sign up

Export Citation Format

Share Document