scholarly journals Antiradical Activity of Dopamine, L-DOPA, Adrenaline, and Noradrenaline in Water/Methanol and in Liposomal Systems

2021 ◽  
Author(s):  
Katarzyna Jodko-Piórecka ◽  
Bożena Sikora ◽  
Monika Kluzek ◽  
Paweł Przybylski ◽  
Grzegorz Litwinienko
Keyword(s):  
Antiradical Activity

Effect of 1,3,6-trimethyl-5-hydroxyuracil succinate on the antioxidant system and free-radical processes in the liver of adult and old rats treated with carbon tetrachloride

2018 ◽  
pp. 55-59
Author(s):  
И.Д. Габдрахманова ◽  
В.А. Мышкин ◽  
Д.А. Еникеев ◽  
А.Р. Гимадиева
Keyword(s):  
Superoxide Dismutase ◽  
Free Radical ◽  
Carbon Tetrachloride ◽  
Antioxidant System ◽  
Complex Compound ◽  
Antiradical Activity ◽  
Conjugated Dienes ◽  
Experimental Animals ◽  
Old Rats ◽  
Radical Processes

Цель исследования: изучение влияния сукцината 1,3,6-триметил-5-гидроксиурацила на антиоксидантную систему и свободнорадикальные процессы в печени взрослых и старых крыс при воздействии тетрахлорметана. Методика. В эксперименте использованы половозрелые животные 12-месячного возраста со средней массой 250 г и старые животные 24-месячного возраста, средней массой 395 г по 30 особей в каждой возрастной группе. Токсическое поражение печени вызывали подкожным введением 50%-ного масляного раствора тетрахлорметана (ТХМ, 2 г/кг) в течение 4 сут. Одновременно с токсикантом опытным животным внутрибрюшинно вводили водный раствор коплексного соединения сукцинат-1,3,6-триметил-5-гидроксиурацила (2,5 мг/100 г) 3 раза в сут. в течение первых 4 сут. и в течение последующих 3 сут. 1 раз в сут. Контролем служили опытные животные, которым вводили физиологический раствор в том же объеме. Изучали окислительную модификацию белков, перекисное окисление липидов (по содержанию ТБК-реагирующих продуктов, уровню гидроперекисей липидов и содержанию диеновых конъюгатов). Состояние антиоксидантной системы оценивали по активности ферментов супероксиддисмутазы, каталазы и глутатионпероксидазы, определяемых биохимическими методами. Антирадикальную активность комплексного соединения и его составляющих субстанций исследовали в модельной системе «этилбензол-ледяная уксусная кислота» с вычислением константы К - скорости взаимодействия перекисных радикалов с молекулами изучаемого соединения в сравнении с эталонным антиоксидантом-ионолом с витамином Е. Результаты. Сукцинат + 1,3,6-триметил-5-гидрокси-урацила существенно снижает токсическое действие ТХМ на печень взрослых и старых крыс, устраняет дисбаланс в системах свободнорадикального окисления белков у старых крыс, статистически значимо улучшает показатели свободнорадикального окисления (СРО) липидов в печени взрослых и старых крыс: снижает уровень продуктов ПОЛ - гидроперекисей, диеновых конъюгатов, ТБК-реагирующих продуктов, а также улучшает работу антиоксидантной системы (АОС), повышая активность каталазы, супероксиддисмутазы и глутатионпероксидазы. Установлена высокая антирадикальная активность изучаемого препарата сопоставимая с активностью эталонного антиоксиданта ионола. Заключение. Сукцинат и его производные способны выступать как индивидуальные вещества с непосредственным антирадикальным механизмом действия, а не только как стимуляторы ферментативных систем антиоксидантной защиты. Aim. To study the effect of a complex compound, 1,3,6-trimethyl-5-hydroxyuracil succinate, on the antioxidant system and free radical processes induced by carbon tetrachloride in the liver of adult and old rats. Methods. The study used sexually mature animals aged 12 months and weighing 250 g and old animals aged 24 months weighing 395 g (total n= 60, 30 rats in each age group). Toxic damage of liver was induced by subcutaneous injections of a 50% oil solution of carbon tetrachloride (CTC) at 2 g/kg for 4 days. Together with the toxicant, experimental animals were injected with a water solution of a complex compound, succinate 1,3,6-trimethyl-5-hydroxyuracil, at a dose of 2.5 mg/100 g, i.p., 3 times per day for the first 4 days and once daily for the following 3 days. Experimental animals were used as controls, which were administered saline in the same volume. Oxidative modifications of proteins, lipid peroxidation (by levels of thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides, and conjugated dienes) were studied. Condition of the antioxidant system was evaluated by activities of superoxide dismutase, catalase, and glutathione peroxidase using biochemical methods. Antiradical activity of the complex compound and its components was studied in a model system of ethylbenzene-glacial acetic acid; the K7 constant of the rate of peroxide radical interaction with molecules of the studied compound was compared with the reference antioxidant ionol with vitamin E. Results. Succinate +1.3.6-trimethyl-5-hydroxyuracil, considerably reduced TXM hepatotoxicity in adult and old rats; removed the disbalance in free radical systems of protein oxidation in old rats; significantly improved indexes of free-radical oxidation (FRO) of hepatic lipids in adult and old rats; decreased levels of LP products, hydroperoxides, conjugated dienes, and TBARS, and enhanced performance of the antioxidant system (AOS) by increasing activities of catalase, superoxide dismutase, and glutathione peroxidase. The study demonstrated a high antiradical activity of the study drug comparable with the activity of the reference antioxidant, ionol. Сonclusion. Succinate and its derivatives can perform as individual substances with a direct antiradical mechanism of action rather than as stimulators of enzymic systems of antioxidant defence.

scholarly journals Comprehensive Study of Variety Oenological Potential Using Statistic Tools for the Efficient Use of Non-Renewable Resources

2021 ◽  
Vol 11 (9) ◽  
pp. 4003
Author(s):  
Sílvia Petronilho ◽  
Alisa Rudnitskaya ◽  
Manuel A. Coimbra ◽  
Sílvia M. Rocha
Keyword(s):  
Renewable Resources ◽  
Antiradical Activity ◽  
Total Phenolics ◽  
Titratable Acidity ◽  
Grape Berry ◽  
Total Sugars ◽  
Components Analysis ◽  
Harvest Year ◽  
Grape Varieties ◽  
Comprehensive Study

The evaluation of the variety suitability regarding each appellation’s specificities should be a strategy for maximizing the varieties’ oenological potential while contributing to the sustainable production of quality wines, keeping their typicity and rationalizing winemaking costs. Thus, the combination of several grape physicochemical attributes, modulated by climate and vineyard characteristics, providing knowledge for each grape variety’s oenological potential, is a relevant and reliable support for winemakers’ decisions. To prove this hypothesis, six mature grape varieties from three harvests, each one from three vineyard parcels with different topographical conditions from Bairrada Appellation (Portugal), were studied using analysis of variance–simultaneous components analysis (ASCA). The effects of harvest year and parcel on grape berry weight, pH, titratable acidity, total sugars, total phenolics, antiradical activity, and volatile composition in free and glycosidically-linked forms were analyzed. The compositional plasticity of autochthonous varieties (white Arinto and Bical and red Baga, Castelão, and Touriga Nacional) was observed. Sauvignon Blanc grape composition was significantly modulated by harvest. This study represents an important contribution for the maintenance of varieties’ biodiversity while contributing to establishing their peculiarities. Autochthonous varieties, if accurately exploited, can provide higher characteristic diversity than worldwide used varieties, an aspect to be more objectively taken into consideration by winemakers.

Note: A Comparative Study on the in Vitro Antiradical Activity and Hydroxyl Free Radical Scavenging Activity in Aged Red Wines

2003 ◽  
Vol 9 (6) ◽  
pp. 383-387 ◽  
Author(s):  
P. Kefalas ◽  
S. Kallithraka ◽  
I. Parejo ◽  
D. P. Makris
Keyword(s):  
Free Radical ◽  
Antiradical Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Red Wines ◽  
Hydroxyl Free Radical ◽  
Important Relationship

The antiradical and hydroxyl free radical scavenging activities were estimated in twenty-five, aged red wines from different areas in Greece. The antiradical activity (AAR) was determined by means of the wellknown DPPH• method, and its values ranged from 24.7 to 125.1. A novel, chemiluminescence-based, highly sensitive assay was applied for determination of the hydroxyl free radical scavenging activity (SAHFR), which varied from 1.62 to 12.22 mM quercetin equivalents. The values from the two assays correlated very well (r2 =0.8542, P<0.001), which confirmed an important relationship between SAHFR and AAR. This tendency in aged red wines, which may be significant in evaluating the antioxidant behaviour of red wine polyphenols, is discussed on the basis of previous research and relevant data.

scholarly journals Phytochemical and Antioxidant Dynamics of the Soursop Fruit (Annona muricata L.) in response to Colletotrichum spp.

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Alejandro Rubio-Melgarejo ◽  
Rosendo Balois-Morales ◽  
Yolotzin Apatzingan Palomino-Hermosillo ◽  
Graciela Guadalupe López-Guzmán ◽  
José Carmen Ramírez-Ramírez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antiradical Activity ◽  
Late Response ◽  
Total Phenols ◽  
Annona Muricata ◽  
Coumaric Acid ◽  
Colletotrichum Siamense ◽  
Soluble Phenols ◽  
Colletotrichum Spp ◽  
Higher Sensitivity

This work evaluates the effect of the pathogens Colletotrichum siamense and C. gloeosporioides on the response of soursop fruits. The bioactive compounds (total phenols, flavonoids, anthraquinones, coumarins, steroids, terpenoids, alkaloids, and saponins) were evaluated qualitatively in soursop pulp. Positive phytochemicals and antioxidant activity (DPPH•, ABTS•+, and FRAP) were quantified at day zero, one, three, and five. Fruits treated with C. gloeosporioides showed higher disease severity (P<0.05). Early fruit response (day one) was observed with both pathogens, increased the concentration of saponins and repressed the production of quercetin 3-O-glucoside (P<0.05). Likewise, C. siamense decreased total soluble phenols and flavonoids and increased antiradical activity DPPH•. Besides, C. gloeosporioides decreased the levels of kaempferol 3-O-rutinoside and ferulic acid (P<0.05). Regarding the late response (day three), both pathogens decreased the concentration of saponins and increased flavonoids and phytosterols (P<0.05). Nevertheless, C. siamense increased the levels of total soluble phenols, p-coumaric acid, kaempferol, and antiradical activity FRAP (P<0.05). Also, C. gloeosporioides repressed the production of quercetin 3-O-glucoside at day five (P<0.05). Soursop fruits had a response to the attack of Colletotrichum during ripening at physicochemical and oxidative levels, which is associated with the production of compounds related to the development inhibition of pathogens. Even so, soursop fruits showed higher susceptibility to C. gloeosporioides and higher sensitivity to the attack of C. siamense.

Influence of hydroxypropyl-β-cyclodextrin on the photostability and antiradical activity of Trolox

2008 ◽  
Vol 61 (3-4) ◽  
pp. 279-287 ◽  
Author(s):  
M. E. Carlotti ◽  
S. Sapino ◽  
S. Marino ◽  
E. Ugazio ◽  
F. Trotta ◽  
...  
Keyword(s):  
Antiradical Activity

scholarly journals Effect of probiotic bacteria on antiradical activity of peptides isolated from dry-cured loins

2017 ◽  
Vol 15 (3) ◽  
pp. 382-390 ◽  
Author(s):  
Anna Okoń ◽  
Joanna Stadnik ◽  
Zbigniew J. Dolatowski
Keyword(s):  
Antiradical Activity ◽  
Probiotic Bacteria

Antioxidant and Antiradical Activity of Hydroxy-Substituted 4-Thiaflavanes

2006 ◽  
Vol 89 (10) ◽  
pp. 2462-2472 ◽  
Author(s):  
Riccardo Amorati ◽  
Maria Grazia Fumo ◽  
Gian Franco Pedulli ◽  
Stefano Menichetti ◽  
Chiara Pagliuca ◽  
...  
Keyword(s):  
Antiradical Activity
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