Heteroaryldihydropyrimidines Alter Capsid Assembly By Adjusting the Binding Affinity and Pattern of the Hepatitis B Virus Core Protein

2019 ◽  
Vol 59 (12) ◽  
pp. 5104-5110 ◽  
Author(s):  
Huihui Liu ◽  
Susumu Okazaki ◽  
Wataru Shinoda
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Binding Affinity ◽  
Core Protein ◽  
Capsid Assembly ◽  
Virus Core ◽  
B Virus

Phosphorylation of hepatitis B virus core C-terminally truncated protein (Cp149) by PKC increases capsid assembly and stability

2008 ◽  
Vol 416 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Hang Kang ◽  
Jaehoon Yu ◽  
Guhung Jung
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Core Protein ◽  
Virus Titre ◽  
Capsid Assembly ◽  
Human Hepatoma Cells ◽  
Virus Core ◽  
C Terminus ◽  
B Virus

The HBV (hepatitis B virus) core is a phosphoprotein whose assembly, replication, encapsidation and localization are regulated by phosphorylation. It is known that PKC (protein kinase C) regulates pgRNA (pregenomic RNA) encapsidation by phosphorylation of the C-terminus of core, which is a component packaged into capsid. Neither the N-terminal residue phosphorylated by PKC nor the role of the C-terminal phosphorylation have been cleary defined. In the present study we found that HBV Cp149 (core protein C-terminally truncated at amino acid 149) expressed in Escherichia coli was phosphorylated by PKC at Ser106. PKC-mediated phosphorylation increased core affinity, as well as assembly and capsid stability. In vitro phosphorylation with core mutants (S26A, T70A, S106A and T114A) revealed that the Ser106 mutation inhibited phosphorylation of core by PKC. CD analysis also revealed that PKC-mediated phosphorylation stabilized the secondary structure of capsid. When either pCMV/FLAG-Cp149[WT (wild-type)] or pCMV/FLAG-S106A Cp149 was transfected into Huh7 human hepatoma cells, mutant capsid level was decreased by 2.06-fold with the S106A mutant when compared with WT, although the same level of total protein was expressed in both cases. In addition, when pUC1.2x and pUC1.2x/S106A were transfected, mutant virus titre was decreased 2.31-fold compared with WT virus titre. In conclusion, PKC-mediated phosphorylation increased capsid assembly, stability and structural stability.

scholarly journals Hepatitis B Virus Core Protein Domains Essential for Viral Capsid Assembly in a Cellular Context

2020 ◽  
Vol 432 (13) ◽  
pp. 3802-3819 ◽  
Author(s):  
Virgile Rat ◽  
Xavier Pinson ◽  
Florian Seigneuret ◽  
Stéphanie Durand ◽  
Charline Herrscher ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Core Protein ◽  
Protein Domains ◽  
Viral Capsid ◽  
Capsid Assembly ◽  
Virus Core ◽  
B Virus ◽  
Cellular Context

scholarly journals The Hepatitis B Virus Core Protein Intradimer Interface Modulates Capsid Assembly and Stability

Biochemistry ◽  
2014 ◽  
Vol 53 (34) ◽  
pp. 5496-5504 ◽  
Author(s):  
Lisa Selzer ◽  
Sarah P. Katen ◽  
Adam Zlotnick
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Core Protein ◽  
Capsid Assembly ◽  
Virus Core ◽  
B Virus

Cetylpyridinium chloride interaction with the hepatitis B virus core protein inhibits capsid assembly

2019 ◽  
Vol 263 ◽  
pp. 102-111 ◽  
Author(s):  
Hyun Wook Seo ◽  
Joon Pyung Seo ◽  
Yuri Cho ◽  
Eunkyong Ko ◽  
Yoon Jun Kim ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Core Protein ◽  
Cetylpyridinium Chloride ◽  
Capsid Assembly ◽  
Virus Core ◽  
B Virus ◽  
Chloride Interaction

Antivirals interacting with hepatitis B virus core protein and core mutations may misdirect capsid assembly in a similar fashion

2003 ◽  
Vol 66 (12) ◽  
pp. 2273-2279 ◽  
Author(s):  
Hans Jörg Hacker ◽  
Karl Deres ◽  
Maria Mildenberger ◽  
Claus H. Schröder
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Core Protein ◽  
Capsid Assembly ◽  
Virus Core ◽  
B Virus

scholarly journals Dominant negative mutants of the duck hepatitis B virus core protein interfere with RNA pregenome packaging and viral DNA synthesis

Hepatology ◽  
1999 ◽  
Vol 30 (1) ◽  
pp. 308-315 ◽  
Author(s):  
Fritz von Weizsäcker ◽  
Josef Köck ◽  
Stefan Wieland ◽  
Wolf-Bernhard Offensperger ◽  
Hubert E. Blum
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Dna Synthesis ◽  
Core Protein ◽  
Dominant Negative ◽  
Viral Dna ◽  
Duck Hepatitis B Virus ◽  
Virus Core ◽  
Duck Hepatitis ◽  
B Virus

scholarly journals APOBEC3G directly binds Hepatitis B virus core protein in cell and cell free systems

2010 ◽  
Vol 151 (2) ◽  
pp. 213-219 ◽  
Author(s):  
Dongjiu Zhao ◽  
Xianfeng Wang ◽  
Guohua Lou ◽  
Guoping Peng ◽  
Jie Li ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Core Protein ◽  
Virus Core ◽  
B Virus

scholarly journals Transbody against virus core protein potently inhibits hepadnavirus replication in vivo : evidence from a duck model of hepatitis B virus

2017 ◽  
Vol 174 (14) ◽  
pp. 2261-2272 ◽  
Author(s):  
Yiping Li ◽  
Zhengwen Liu ◽  
Lingyun Hui ◽  
Xi Liu ◽  
Ai Feng ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Core Protein ◽  
Virus Core ◽  
B Virus
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