Discovery of a Novel HIV-1 Integrase/p75 Interacting Inhibitor by Docking Screening, Biochemical Assay, and in Vitro Studies

2017 ◽  
Vol 57 (9) ◽  
pp. 2336-2343 ◽  
Author(s):  
Yan Wang ◽  
Huang-Quan Lin ◽  
Ping Wang ◽  
Jian-Shu Hu ◽  
Tsz-Ming Ip ◽  
...  
Keyword(s):  
In Vitro Studies ◽  
Biochemical Assay ◽  
Hiv 1

Focus on the therapeutic efficacy of 3BNC117 against HIV-1: In vitro studies, in vivo studies, clinical trials and challenges

2017 ◽  
Vol 52 ◽  
pp. 44-50 ◽  
Author(s):  
Zhi-Jun Liu ◽  
Jing Bai ◽  
Feng-Li Liu ◽  
Xiang-Yang Zhang ◽  
Jing-Zhang Wang
Keyword(s):  
Clinical Trials ◽  
Therapeutic Efficacy ◽  
In Vitro Studies ◽  
In Vivo Studies ◽  
Hiv 1

In vitro studies of HIV-1 expression in thymocytes from infants and children

AIDS ◽  
1992 ◽  
Vol 6 (3) ◽  
pp. 265-272 ◽  
Author(s):  
Esther F. Hays ◽  
Christel H. Uittenbogaart ◽  
John C. Brewer ◽  
Leanne W. Vollger ◽  
Jerome A. Zack
Keyword(s):  
In Vitro Studies ◽  
Infants And Children ◽  
Hiv 1

In Vitro Studies of HIV-1 Infection in Thymic Lymphocytes: A Putative Role of the Thymus in AIDS Pathogenesis

1990 ◽  
Vol 6 (3) ◽  
pp. 287-298 ◽  
Author(s):  
ANITA De ROSSI ◽  
MARIA LUISA CALABRO ◽  
MARINA PANOZZO ◽  
DANIELE BERNARDI ◽  
BEATRICE CARUSO ◽  
...  
Keyword(s):  
In Vitro Studies ◽  
Putative Role ◽  
Hiv 1

scholarly journals HIV gene expression deactivates redox-sensitive stress response program in mouse tubular cells both in vitro and in vivo

2012 ◽  
Vol 302 (1) ◽  
pp. F129-F140 ◽  
Author(s):  
Divya Salhan ◽  
Shresh Pathak ◽  
Mohammad Husain ◽  
Pranai Tandon ◽  
Dileep Kumar ◽  
...  
Keyword(s):  
Free Radical ◽  
Stress Response ◽  
Cell Apoptosis ◽  
In Vitro Studies ◽  
Tubular Cell ◽  
Ros Generation ◽  
Tubular Cells ◽  
Hiv 1

Human immunodeficiency virus (HIV)-1 has been reported to cause tubular cell injury both in in vivo and in vitro studies. In the present study, we evaluated the role of oxidative stress in the induction of apoptosis in HIV gene expressing mouse tubular cells in in vivo (Tg26, a transgenic mouse model of HIV-associated nephropathy) and in vitro (tubular cells were transduced with pNL4-3: ΔG/P-GFP, VSV.G psueudo typed virus) studies. Although Tg26 mice showed enhanced tubular cell reactive oxygen species (ROS) generation and apoptosis, renal tissue did not display a robust antioxidant response in the form of enhanced free radical scavenger (MnSOD/catalase) expression. Tg26 mice not only showed enhanced tubular cell expression of phospho-p66ShcA but also displayed nuclear Foxo3a translocation to the cytoplasm. These findings indicated deactivation of tubular cell Foxo3A-dependent redox-sensitive stress response program (RSSRP) in Tg26 mice. In in vitro studies, NL4-3 (pNL4-3: ΔG/P-GFP, VSV.G pseudotyped virus)-transduced mouse proximal tubular cells (NL4-3/MPTEC) displayed enhanced phosphorylation of p66ShcA. NL4-3/MPTECs also displayed greater ( P < 0.01) ROS generation when compared with empty vector-transduced tubular cells; however, both diminution of p66ShcA and N-acetyl cysteine attenuated NL4-3-induced tubular cell ROS generation as well as apoptosis. In addition, both antioxidants and free radical scavengers partially inhibited HIV-induced tubular cell apoptosis. NL4-3/MPTEC displayed deactivation of RSSRP in the form of enhanced phosphorylation of Foxo3A and attenuated expression of superoxide dismutase (SOD) and catalase. Since both SOD and catalase were able to provide protection against HIV-1-induced tubular cell apoptosis, it suggests that HIV-1-induced proapoptotic effect may be a consequence of the deactivated RSSRP.

Inhibition of HIV-1 gp41 expression with hammerhead ribozymes

2015 ◽  
Vol 471 (1) ◽  
pp. 53-66 ◽  
Author(s):  
Agnieszka Fedoruk-Wyszomirska ◽  
Maciej Szymański ◽  
Paweł Głodowicz ◽  
Marta Gabryelska ◽  
Eliza Wyszko ◽  
...  
Keyword(s):  
Hammerhead Ribozyme ◽  
In Vitro Studies ◽  
Hammerhead Ribozymes ◽  
Intracellular Activity ◽  
Highly Effective ◽  
Hiv 1

We propose a new highly effective hammerhead ribozyme against HIV-1 gp41 that can be potentially used as a basis in HIV-1 therapy. We demonstrate that the ribozyme's intracellular activity cannot be inferred solely from in vitro studies.

Targeted femtosecond laser driven drug delivery within HIV-1 infected cells: in-vitro studies

2017 ◽  
Author(s):  
Charles Maphanga ◽  
Saturnin Ombinda-Lemboumba ◽  
Sello Manoto ◽  
Malik Maaza ◽  
Patience Mthunzi-Kufa
Keyword(s):  
Drug Delivery ◽  
Femtosecond Laser ◽  
In Vitro Studies ◽  
Infected Cells ◽  
Hiv 1

In vitro - studies with antler bone cells: Structure forming capacity, osteocalcin production and influence of sex steroids

2006 ◽  
Vol 15 (04) ◽  
pp. 245-257 ◽  
Author(s):  
H. J. Rolf ◽  
K. G. Wiese ◽  
H. Siggelkow ◽  
H. Schliephake ◽  
G. A. Bubernik
Keyword(s):  
Sex Steroids ◽  
Bone Cells ◽  
In Vitro Studies ◽  
Osteocalcin Production
Sign in / Sign up

Export Citation Format

Share Document