scholarly journals Mechanism of Dynamic Binding of Replication Protein A to ssDNA

2020 ◽  
Vol 60 (10) ◽  
pp. 5057-5069
Author(s):  
Anupam Mondal ◽  
Arnab Bhattacherjee
Keyword(s):  
Protein A ◽  
Replication Protein A ◽  
Replication Protein ◽  
Dynamic Binding

scholarly journals Dynamic binding of replication protein a is required for DNA repair

2016 ◽  
Vol 44 (12) ◽  
pp. 5758-5772 ◽  
Author(s):  
Ran Chen ◽  
Shyamal Subramanyam ◽  
Adrian H. Elcock ◽  
Maria Spies ◽  
Marc S. Wold
Keyword(s):  
Dna Repair ◽  
Protein A ◽  
Replication Protein A ◽  
Replication Protein ◽  
Dynamic Binding

scholarly journals APOBEC1 cytosine deaminase activity on single-stranded DNA is suppressed by replication protein A

2020 ◽  
Author(s):  
Lai Wong ◽  
Frederick S Vizeacoumar ◽  
Franco J Vizeacoumar ◽  
Linda Chelico
Keyword(s):  
Genomic Dna ◽  
Cytidine Deaminase ◽  
Protein A ◽  
Cytosine Deaminase ◽  
Cancer Cell Line ◽  
Replication Protein A ◽  
Lung Cancer Cell Line ◽  
Replication Protein ◽  
Cancer Evolution

Abstract Many APOBEC cytidine deaminase members are known to induce ‘off-target’ cytidine deaminations in 5′TC motifs in genomic DNA that contribute to cancer evolution. In this report, we characterized APOBEC1, which is a possible cancer related APOBEC since APOBEC1 mRNA is highly expressed in certain types of tumors, such as lung adenocarcinoma. We found a low level of APOBEC1-induced DNA damage, as measured by γH2AX foci, in genomic DNA of a lung cancer cell line that correlated to its inability to compete in vitro with replication protein A (RPA) for ssDNA. This suggests that RPA can act as a defense against off-target deamination for some APOBEC enzymes. Overall, the data support the model that the ability of an APOBEC to compete with RPA can better predict genomic damage than combined analysis of mRNA expression levels in tumors and analysis of mutation signatures.

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