The Effect of Temperature on Different Aqueous Two-Phase Diagrams of Polyethylene Glycol (PEG 6000, PEG 8000, and PEG 10000) + Potassium Sodium Tartrate + Water

2019 ◽  
Vol 64 (7) ◽  
pp. 3036-3043 ◽  
Author(s):  
Jacqueline Herbst ◽  
Robert W. M. Pott
Keyword(s):  
Polyethylene Glycol ◽  
Phase Diagrams ◽  
Effect Of Temperature ◽  
Two Phase ◽  
Sodium Tartrate ◽  
Peg 6000 ◽  
Potassium Sodium ◽  
Peg 8000 ◽  
Potassium Sodium Tartrate

scholarly journals Use of Aqueous Two-Phase and Three-Phase Partitioning Systems for Purification of Lipase Obtained in Solid-State Fermentation by Rhizopus arrhizus

2019 ◽  
Vol 13 (1) ◽  
pp. 27-36 ◽  
Author(s):  
Valentina Dobreva ◽  
Boriana Zhekova ◽  
Georgi Dobrev
Keyword(s):  
Solid State ◽  
Ammonium Sulfate ◽  
Rhizopus Arrhizus ◽  
Two Phase ◽  
Sodium Tartrate ◽  
Phase Partitioning ◽  
Potassium Sodium ◽  
Three Phase ◽  
Ammonium Sulfate Saturation ◽  
Potassium Sodium Tartrate

Background: Purification of enzymes by conventional methods such as precipitation and chromatographic techniques is a costly and time-consuming procedure and may lead to low yields of enzyme activity. Alternative liquid-liquid extraction methods such as Aqueous Two-Phase Systems (ATPS) and Three Phase Partitioning (TPP) are characterized by the high enzyme yields and purification degree. Objective: The objective of this study was the application of partitioning systems ATPS and TPP for purification of lipase produced in solid-state fermentation by Rhizopus arrhizus. Methods: ATPS and TPP were used for purification of lipase, obtained by solid state cultivation of Rhizopus arrhizus. Results: Lipase was isolated with PEG4000/potassium sodium tartrate ATPS and the effect of the system composition, including PEG 4000 and potassium sodium tartrate concentrations on lipase partitioning was studied. When using 30% PEG4000/21% potassium sodium tartrate, lipase was distributed in the top phase, and the highest recovery yield of 217% and purification fold of 6.1 were achieved. It was found that at PEG4000 concentration of or higher than 15%, the enzyme was present in the top polymer-rich phase with a partitioning yield of over 90%. Upon application of TPP for lipase isolation, the effect of t-butanol concentration, ammonium sulfate concentration and pH on enzyme partitioning was investigated. The highest lipase recovery yield of 71% and 19.1-fold purification were achieved in the interfacial phase in the presence of 30% ammonium sulfate saturation with 1.0:0.5 crude extract/t-butanol ratio at pH 7 in a single step. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis and zymographic analysis showed significant purification of lipase by TPP and the presence of two multiple forms of the enzyme. Conclusion: ATPS (PEG4000/ Potassium sodium tartrate) and TPP (1.0:0.5 crude extract/t-butanol ratio, 30% ammonium sulfate saturation, pH 7) proved to be rapid methods for the isolation and purification of lipase and they can be used in downstream processing for industrial preparation of the enzyme.

scholarly journals The Corrosion Inhibition Performance of Polyacrylic Acid with Potassium Sodium Tartrate and Zn2+ for Corrosion Control of Mild Steel in Aqueous Solution

2015 ◽  
Vol 61 ◽  
pp. 137-148 ◽  
Author(s):  
V. Dharmalingam ◽  
P. Arockia Sahayaraj ◽  
A. John Amalraj ◽  
R. Shobana ◽  
R. Mohan
Keyword(s):  
Mild Steel ◽  
Corrosion Inhibition ◽  
Polyacrylic Acid ◽  
Potentiodynamic Polarization ◽  
Electrochemical Impedance ◽  
Aqueous Environment ◽  
Protective Film ◽  
Sodium Tartrate ◽  
Potassium Sodium ◽  
Potassium Sodium Tartrate

The goal of studying corrosion process is to find means of minimizing corrosion or prevent it from occurring. The use of inhibitors is one of the most popular methods for corrosion protection. A protective film has been formed on the surface of the mild steel in a neutral aqueous environment using a synergistic mixture of an eco-friendly inhibitor viz., Potassium Sodium Tartrate (SPT) along with polyacrylic acid (PAA) and Zn2+ ions. The inhibiting effect of SPT, PAA and Zn2+ ions have been investigated by gravimetric studies, Potentiodynamic polarization and electrochemical impedance spectroscopy (EIS). The investigations revealed that SPT acts as an excellent synergist in corrosion inhibition. Optimum concentrations of all the three components of the ternary formulation are established by gravimetric studies. Potentiodynamic polarization studies inferred that this mixture functions as a cathodic inhibitor. EIS studies of the metal/solution interface indicated that the surface film is highly protective against the corrosion of mild steel. Surface characterization techniques (FTIR, SEM, AFM) are also used to ascertain the nature of the protective film. The mechanical aspect of corrosion inhibition is proposed.

Aqueous two-phase system (polyethylene glycol + ionic liquid) for extraction of α-amylase: phase diagrams, systems characterization and partition study

2020 ◽  
Vol 37 (3) ◽  
pp. 595-606
Author(s):  
O. R. R. Gandolfi ◽  
G. R. F. Gonçalves ◽  
I. C. Batista ◽  
R. C. I. Fontan ◽  
C. M. Veloso ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Polyethylene Glycol ◽  
Phase Diagrams ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System
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