Biochemical Characterization and Structural Analysis of a β-N-Acetylglucosaminidase from Paenibacillus barengoltzii for Efficient Production of N-Acetyl-d-glucosamine

2020 ◽  
Vol 68 (20) ◽  
pp. 5648-5657 ◽  
Author(s):  
Yihao Liu ◽  
Zhengqiang Jiang ◽  
Junwen Ma ◽  
Shuai Ma ◽  
Qiaojuan Yan ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Biochemical Characterization ◽  
Efficient Production ◽  
Paenibacillus Barengoltzii

scholarly journals Discovery and characterization of a novel family of prokaryotic nanocompartments involved in sulfur metabolism

2020 ◽  
Author(s):  
Robert J. Nichols ◽  
Benjamin LaFrance ◽  
Naiya R. Phillips ◽  
Luke M. Oltrogge ◽  
Luis E. Valentin-Alvarado ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Biochemical Characterization ◽  
Sulfur Metabolism ◽  
Physiological Role ◽  
Enzymatic Characterization ◽  
Cysteine Desulfurase ◽  
Future Studies ◽  
Pcc 7942

AbstractProkaryotic nanocompartments, also known as encapsulins, are a recently discovered proteinaceous organelle in prokaryotes that compartmentalize cargo enzymes. While initial studies have begun to elucidate the structure and physiological roles of encapsulins, bioinformatic evidence suggests that a great diversity of encapsulin nanocompartments remains unexplored. Here, we describe a novel encapsulin in the freshwater cyanobacterium Synechococcus elongatus PCC 7942. This nanocompartment is upregulated upon sulfate starvation and encapsulates a cysteine desulfurase enzyme via an N-terminal targeting sequence. Using cryoelectron microscopy, we have determined the structure of the nanocompartment complex to 2.2 Å resolution. Lastly, biochemical characterization of the complex demonstrated that the activity of the cysteine desulfurase is enhanced upon encapsulation. Taken together, our discovery, structural analysis, and enzymatic characterization of this prokaryotic nanocompartment provide a foundation for future studies seeking to understand the physiological role of this encapsulin in various bacteria.

Biochemical characterization of a truncated β-agarase from Microbulbifer sp. suitable for efficient production of neoagarotetraose

2019 ◽  
Vol 87 ◽  
pp. 119-127 ◽  
Author(s):  
Junwen Ma ◽  
Qiaojuan Yan ◽  
Ping Yi ◽  
Shaoqing Yang ◽  
Haijie Liu ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Efficient Production

Purification and biochemical characterization of xanthopterin from patients with chronic renal failure. II. Biochemical elucidation and structural analysis

1991 ◽  
Vol 24 (5) ◽  
pp. 407-415 ◽  
Author(s):  
Robert H. Williams ◽  
Mashouf Shaykh ◽  
Sarosh Ahmed ◽  
Theodore Musiala ◽  
Nadine Bazilinski ◽  
...  
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Structural Analysis ◽  
Biochemical Characterization

Biochemical characterization of a novel xylanase from Paenibacillus barengoltzii and its application in xylooligosaccharides production from corncobs

2018 ◽  
Vol 264 ◽  
pp. 310-318 ◽  
Author(s):  
Xueqiang Liu ◽  
Yu Liu ◽  
Zhengqiang Jiang ◽  
Haijie Liu ◽  
Shaoqing Yang ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Paenibacillus Barengoltzii

scholarly journals Mutagenesis, biochemical characterization and X-ray structural analysis of point mutants of bovine chymosin

1997 ◽  
Vol 10 (9) ◽  
pp. 991-997 ◽  
Author(s):  
M. G. Williams ◽  
J. Wilsher ◽  
P. Nugent ◽  
A. Mills ◽  
V. Dhanaraj ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Biochemical Characterization ◽  
X Ray

scholarly journals Dimerization of long hibernation promoting factor from Staphylococcus aureus: Structural analysis and biochemical characterization

2020 ◽  
Vol 209 (1) ◽  
pp. 107408 ◽  
Author(s):  
Konstantin S. Usachev ◽  
Bulat F. Fatkhullin ◽  
Evelina A. Klochkova ◽  
Aynur K. Miftakhov ◽  
Alexander A. Golubev ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Structural Analysis ◽  
Biochemical Characterization

Biochemical characterization and structural analysis of trypsin from Plodia interpunctella midgut: implication of determinants in extremely alkaline pH activity profile

2017 ◽  
Vol 42 (4) ◽  
pp. 307-318 ◽  
Author(s):  
Seyed A. Hemmati ◽  
Reza H. Sajedi ◽  
Saeid Moharramipour ◽  
Majid Taghdir ◽  
Hossein Rahmani ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Biochemical Characterization ◽  
Plodia Interpunctella ◽  
Alkaline Ph ◽  
Activity Profile

Cloning, functional expression, biochemical characterization, and structural analysis of a haloalkane dehalogenase from Plesiocystis pacifica SIR-1

2011 ◽  
Vol 91 (4) ◽  
pp. 1049-1060 ◽  
Author(s):  
Martin Hesseler ◽  
Xenia Bogdanović ◽  
Aurelio Hidalgo ◽  
Jose Berenguer ◽  
Gottfried J. Palm ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Biochemical Characterization ◽  
Functional Expression ◽  
Haloalkane Dehalogenase
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