Asparanin A from Asparagus officinalis L. Induces G0/G1 Cell Cycle Arrest and Apoptosis in Human Endometrial Carcinoma Ishikawa Cells via Mitochondrial and PI3K/AKT Signaling Pathways

2019 ◽  
Vol 68 (1) ◽  
pp. 213-224 ◽  
Author(s):  
Fan Zhang ◽  
Yuan-Yuan Zhang ◽  
Ya-Sai Sun ◽  
Run-Hui Ma ◽  
Kiran Thakur ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Endometrial Carcinoma ◽  
Cell Cycle Arrest ◽  
Signaling Pathways ◽  
Akt Signaling ◽  
Asparagus Officinalis ◽  
Cycle Arrest ◽  
Ishikawa Cells ◽  
G1 Cell Cycle Arrest

scholarly journals Atorvastatin Calcium Inhibits PDGF-ββ-Induced Proliferation and Migration of VSMCs Through the G0/G1 Cell Cycle Arrest and Suppression of Activated PDGFRβ-PI3K-Akt Signaling Cascade

2017 ◽  
Vol 44 (1) ◽  
pp. 215-228 ◽  
Author(s):  
Shuang Chen ◽  
Siyuan Dong ◽  
Zhao Li ◽  
Xiaofan Guo ◽  
Naijin Zhang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Akt Signaling ◽  
Nuclear Antigen ◽  
Signaling Cascade ◽  
Atorvastatin Calcium ◽  
Cycle Arrest ◽  
G1 Cell Cycle Arrest ◽  
And Migration ◽  
Proliferation And Migration

Background/Aims: Abnormal proliferation of vascular smooth muscle cells (VSMCs) is a hallmark of vascular lesions, such as atherosclerosis and restenosis. PDGF-ββ, an isoform of PDGF (platelet-derived growth factor), has been demonstrated to induce proliferation and migration of VSMCs. Atorvastatin calcium, a selective inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, has favorable protective effects on VSMCs. This study examined the effects of atorvastatin calcium on the proliferation and migration of PDGF-ββ-treated VSMCs, as well as its underlying mechanisms. Methods: MTT assays, Edu imaging, cell cycle analysis, wound healing assays, transwell migration assays, and western blot analysis were performed. Results: Atorvastatin calcium significantly inhibited cell proliferation, DNA synthesis and cell migration of PDGF-ββ-treated VSMCs. We demonstrated that atorvastatin calcium induced cell cycle arrest in the G0/G1 phase in response to PDGF-ββ stimulation and decreased the expression of G0/G1-specific regulatory proteins, including proliferating cell nuclear antigen (PCNA), CDK2, cyclin D1, cyclin E and CDK4 in PDGF-ββ-treated VSMCs. Moreover, pretreatment with atorvastatin calcium inhibited the PDGF-ββ-treated phosphorylation of PDGFRβ and Akt, whereas atorvastatin calcium did not affect the phosphorylation of PLC-γ1 or (ERK) 1/2. Conclusion: Our data suggested that atorvastatin calcium inhibited abnormal proliferation and migration of VSMCs through G0/G1 cell cycle arrest and suppression of the PDGFRβ-Akt signaling cascade.

scholarly journals GNA14 silencing suppresses the proliferation of endometrial carcinoma cells through inducing apoptosis and G2/M cell cycle arrest

2018 ◽  
Vol 38 (5) ◽  
Author(s):  
Jing Wang ◽  
Xiao Lv ◽  
Feixue Xu ◽  
Min Wei ◽  
Cuiping Liu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Endometrial Carcinoma ◽  
Cell Cycle Arrest ◽  
Caspase 3 ◽  
Protein Subunit ◽  
M Cell ◽  
Guanine Nucleotide Binding Protein ◽  
Gynecological Malignancy ◽  
Cycle Arrest ◽  
Ishikawa Cells

Endometrial carcinoma is the most common gynecological malignancy. The pathological factors triggering this disease are largely unknown. Although the role of guanine nucleotide-binding protein subunit α (GNA) 11 (GNA11) in melanoma has been described, the involvement of GNA14 in endometrial carcinoma remains to be determined. Here, we found that GNA14 expression was increased in endometrial carcinoma tissues compared with simple hyperplasia tissues. Based on lentivirus-mediated knockdown assay, we showed that GNA14 silencing significantly suppressed the proliferation of both HEC-1-A and Ishikawa cells. The caspase 3/caspase 7 activity and apoptosis were enhanced by GNA14 knockdown. GNA14 depletion led to cell cycle arrest at the G2/M phase. In addition, Apoptosis Array analysis revealed that caspase-3 and Fas were up-regulated by GNA14 knockdown. Our study suggests that GNA14 silencing blunts endometrial carcinoma cell proliferation. Targetting GNA14 may bring help for the patients of endometrial carcinoma.

scholarly journals Oxaliplatin Induces Cell Cycle Arrest and Apoptosis in T-leukemia Cells through Mitochondrial and PI3K/Akt Signaling Pathways

2021 ◽  
Vol 5 (2) ◽  
pp. 210003-210003
Author(s):  
Mengcheng Li ◽  
Yongpeng Wang ◽  
Lei Tian ◽  
Afsar Khan ◽  
Yudan Wang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Signaling Pathways ◽  
Akt Signaling ◽  
Leukemia Cells ◽  
Cycle Arrest

Signaling pathways involved in cell cycle arrest during the DNA breaks

DNA Repair ◽  
2021 ◽  
Vol 98 ◽  
pp. 103047
Author(s):  
Fatemeh Sadoughi ◽  
Jamal Hallajzadeh ◽  
Zatollah Asemi ◽  
Mohammad Ali Mansournia ◽  
Forough Alemi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Signaling Pathways ◽  
Dna Breaks ◽  
Cycle Arrest
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