Ethylene Perception Is Associated with Methyl-Jasmonate-Mediated Immune Response against Botrytis cinerea in Tomato Fruit

2019 ◽  
Vol 67 (24) ◽  
pp. 6725-6735 ◽  
Author(s):  
Wenqing Yu ◽  
Mengmeng Yu ◽  
Ruirui Zhao ◽  
Jiping Sheng ◽  
Yujing Li ◽  
...  
Keyword(s):  
Immune Response ◽  
Methyl Jasmonate ◽  
Botrytis Cinerea ◽  
Tomato Fruit

SlMYC2 are required for methyl jasmonate-induced tomato fruit resistance to Botrytis cinerea

2020 ◽  
Vol 310 ◽  
pp. 125901 ◽  
Author(s):  
Dedong Min ◽  
Fujun Li ◽  
Xixi Cui ◽  
Jingxiang Zhou ◽  
Jiaozhuo Li ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Botrytis Cinerea ◽  
Tomato Fruit

scholarly journals Infection Strategies Deployed by Botrytis cinerea, Fusarium acuminatum, and Rhizopus stolonifer as a Function of Tomato Fruit Ripening Stage

2019 ◽  
Vol 10 ◽  
Author(s):  
Stefan Petrasch ◽  
Christian J. Silva ◽  
Saskia D. Mesquida-Pesci ◽  
Karina Gallegos ◽  
Casper van den Abeele ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Fruit Ripening ◽  
Tomato Fruit ◽  
Rhizopus Stolonifer ◽  
Ripening Stage ◽  
Fusarium Acuminatum ◽  
Tomato Fruit Ripening

scholarly journals Comparative Proteomics Reveals the Potential Targets of BcNoxR, a Putative Regulatory Subunit of NADPH Oxidase of Botrytis cinerea

2016 ◽  
Vol 29 (12) ◽  
pp. 990-1003 ◽  
Author(s):  
Hua Li ◽  
Zhanquan Zhang ◽  
Chang He ◽  
Guozheng Qin ◽  
Shiping Tian
Keyword(s):  
Nadph Oxidase ◽  
Botrytis Cinerea ◽  
Proteomic Analysis ◽  
Fungal Pathogens ◽  
Molecular Mechanisms ◽  
Quantitative Polymerase Chain Reaction ◽  
Tomato Fruit ◽  
Polymerase Chain Reaction Analysis ◽  
Regulatory Subunit ◽  
Wild Type

The NADPH oxidase (NOX) complex has been shown to play a crucial role in stress response and in the virulence of various fungal pathogens. The underlying molecular mechanisms of NOX, however, remain largely unknown. In the present study, a comparative proteomic analysis compared changes in protein abundance in wild-type Botrytis cinerea and ΔbcnoxR mutants in which the regulatory subunit of NOX was deleted. The ΔbcnoxR mutants exhibited reduced growth, sporulation, and impaired virulence. A total of 60 proteins, representing 49 individual genes, were identified in ΔbcnoxR mutants that exhibited significant differences in abundance relative to wild-type. Reverse transcription-quantitative polymerase chain reaction analysis demonstrated that the differences in transcript levels for 36 of the genes encoding the identified proteins were in agreement with the proteomic analysis, while the remainder exhibited reverse levels. Functional analysis of four proteins that decreased abundance in the ΔbcnoxR mutants indicated that 6-phosphogluconate dehydrogenase (BcPGD) played a role in the growth and sporulation of B. cinerea. The Δbcpgd mutants also displayed impaired virulence on various hosts, such as apple, strawberry, and tomato fruit. These results suggest that NOX can influence the expression of BcPGD, which has an impact on growth, sporulation, and virulence of B. cinerea.

SlMYC2 Involved in Methyl Jasmonate-Induced Tomato Fruit Chilling Tolerance

2018 ◽  
Vol 66 (12) ◽  
pp. 3110-3117 ◽  
Author(s):  
Dedong Min ◽  
Fujun Li ◽  
Xinhua Zhang ◽  
Xixi Cui ◽  
Pan Shu ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Tomato Fruit ◽  
Chilling Tolerance

scholarly journals Priming of Defense Systems and Upregulation of MYC2 and JAZ1 Genes after Botrytis cinerea Inoculation in Methyl Jasmonate-Treated Strawberry Fruits

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 447 ◽  
Author(s):  
Felipe Valenzuela-Riffo ◽  
Paz E. Zúñiga ◽  
Luis Morales-Quintana ◽  
Mauricio Lolas ◽  
Marcela Cáceres ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Botrytis Cinerea ◽  
Defense Responses ◽  
Gray Mold ◽  
The Past ◽  
Defense Systems ◽  
Meja Treatment ◽  
Fruit Development And Ripening ◽  
Genes Encoding ◽  
And Control

Several attempts have been made to study the effects of methyl jasmonate (MeJA) on plants in the past years. However, the comparative effects of the number and phenological time of MeJA applications on the activation of defense systems is currently unknown in strawberries. In the present research, we performed three field treatments during strawberry (Fragaria × ananassa ‘Camarosa’) fruit development and ripening which consisted of differential MeJA applications at flowering (M3), and the large green (M2 and M3) and red ripe (M1, M2, and M3) fruit stages. We also checked changes in gene expression related to plant defense against Botrytis cinerea inoculation post-harvest. In M3 treatment, we observed an upregulation of the anthocyanin and lignin contents and the defense-related genes, encoding for chitinases, β-1,3-glucanases and polygalacturonase-inhibiting proteins, after harvest (0 hpi), along with the jasmonate signaling-related genes FaMYC2 and FaJAZ1 at 48 h after B. cinerea inoculation (48 hpi) during postharvest storage. Although we did not find differences in gray mold incidence between the MeJA treatments and control, these results suggest that preharvest MeJA treatment from the flowering stage onwards (M3) primes defense responses mediated by the upregulation of different defense-related genes and retains the upregulation of MYC2 and JAZ1 at 48 hpi.

scholarly journals Characterization of Botrytis cinerea Isolates from Strawberry with Reduced Sensitivity to Polyoxin D Zinc Salt

Plant Disease ◽  
2016 ◽  
Vol 100 (10) ◽  
pp. 2057-2061 ◽  
Author(s):  
Madeline E. Dowling ◽  
Meng-Jun Hu ◽  
Linus T. Schmitz ◽  
Jennifer R. Wilson ◽  
Guido Schnabel
Keyword(s):  
United States ◽  
South Carolina ◽  
Botrytis Cinerea ◽  
Tomato Fruit ◽  
Resistance Management ◽  
The United States ◽  
Gray Mold ◽  
Zinc Salt ◽  
Malt Extract ◽  
Action Committee

Polyoxin D is a Fungicide Resistance Action Committee (FRAC) code 19 fungicide that was recently registered for gray mold control of strawberry in the United States. In this study, we determined the sensitivity to polyoxin D zinc salt (hereafter, polyoxin D) of Botrytis cinerea isolates from 41 commercial strawberry farms in South Carolina, North Carolina, Maryland, Virginia, and Ohio and investigated the fitness of sensitive (S) and reduced sensitive (RS) isolates. Relative mycelial growth ranged between 0 and over 100% on malt extract agar amended with a discriminatory dose of polyoxin D at 5 μg/ml. Isolates that grew more than 70% at that dose were designated RS and were found in three of the five states. The 50% effective dose (EC50) values of three S and three RS isolates ranged from 0.59 to 2.27 and 4.6 to 5.8 μg/ml, respectively. The three RS isolates grew faster on detached tomato fruit treated with Ph-D WDG at recommended label dosage than S isolates (P < 0.008). In all, 25 randomly selected RS isolates exhibited reduced sporulation ability (P < 0.0001) and growth rate (P < 0.0001) but increased production of sclerotia (P < 0.0386) compared with 25 S isolates. Of 10 isolates tested per phenotype, the number of RS isolates producing sporulating lesions on apple, tomato, and strawberry was significantly lower compared with S isolates (P < 0.0001 for each fruit type). The results of this study indicate that resistance management is necessary for fungicides containing polyoxin D. To our knowledge, this is the first study demonstrating reduced sensitivity to FRAC 19 fungicides in B. cinerea isolates from the United States.

Effect of methyl jasmonate on properties of intact tomato fruit monitored with destructive and nondestructive tests

2007 ◽  
Vol 80 (4) ◽  
pp. 1086-1095 ◽  
Author(s):  
Arturo Baltazar ◽  
Javier Espina-Lucero ◽  
Isidro Ramos-Torres ◽  
Gustavo González-Aguilar
Keyword(s):  
Methyl Jasmonate ◽  
Tomato Fruit ◽  
Nondestructive Tests
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