Metabolite Identification and Pharmacokinetic Profiling of Isoflavones from Black Soybean in Rats Using Ultrahigh-Performance Liquid Chromatography with Linear-Ion-Trap–Orbitrap and Triple-Quadrupole Tandem Mass Spectrometry

2018 ◽  
Vol 66 (49) ◽  
pp. 12941-12952 ◽  
Author(s):  
Juping Zhang ◽  
Qianxiang Guo ◽  
Meijin Wei ◽  
Junqi Bai ◽  
Juan Huang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Ion Trap ◽  
Metabolite Identification ◽  
Linear Ion Trap ◽  
Tandem Mass ◽  
Triple Quadrupole ◽  
Black Soybean ◽  
Ultrahigh Performance Liquid Chromatography

scholarly journals Screening of Drugs and Toxic Compounds with Liquid Chromatography-Linear Ion Trap Tandem Mass Spectrometry

2006 ◽  
Vol 52 (9) ◽  
pp. 1735-1742 ◽  
Author(s):  
François-Ludovic Sauvage ◽  
Franck Saint-marcoux ◽  
Bénédicte Duretz ◽  
Didier Deporte ◽  
Gérard Lachatre ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Mass Spectrometer ◽  
Ion Trap ◽  
Linear Ion Trap ◽  
Tandem Mass ◽  
Triple Quadrupole ◽  
Toxic Compounds ◽  
Quadrupole Linear Ion Trap

Abstract Background: In clinical and forensic toxicology, general unknown screening is used to detect and identify exogenous compounds. In this study, we aimed to develop a comprehensive general unknown screening method based on liquid chromatography coupled with a hybrid triple-quadrupole linear ion trap mass spectrometer. Methods: After solid-phase extraction, separation was performed using gradient reversed-phase chromatography. The mass spectrometer was operated in the information-dependent acquisition mode, switching between a survey scan acquired in the Enhanced Mass Spectrometry mode with dynamic subtraction of background noise and a dependent scan obtained in the enhanced product ion scan mode. The complete cycle time was 1.36 s. A library of 1000 enhanced product ion–tandem mass spectrometry spectra in positive mode and 250 in negative mode, generated using 3 alternated collision tensions during each scan, was created by injecting pure solutions of drugs and toxic compounds. Results: Comparison with HPLC-diode array detection and gas chromatography-mass spectrometry for the analysis of 36 clinical samples showed that linear ion trap tandem mass spectrometry could identify most of the compounds (94% of the total). Some compounds were detected only by 1 of the other 2 techniques. Specific clinical cases highlighted the advantages and limitations of the method. Conclusion: A unique combination of new operating modes provided by hybrid triple-quadrupole linear ion trap mass spectrometers and new software features allowed development of a comprehensive and efficient method for the general unknown screening of drugs and toxic compounds in blood or urine.

scholarly journals Mycotoxin Incidence in Some Fish Products: QuEChERS Methodology and Liquid Chromatography Linear Ion Trap Tandem Mass Spectrometry Approach

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 527 ◽  
Author(s):  
Josefa Tolosa ◽  
Francisco Barba ◽  
Guillermina Font ◽  
Emilia Ferrer
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Ion Trap ◽  
Raw Materials ◽  
Fish Farming ◽  
Linear Ion Trap ◽  
Tandem Mass ◽  
Fish Products ◽  
Fish Samples

The inclusion of vegetal raw materials in feed for fish farming has increased the risk of mycotoxin occurrence in feed, as well as in edible tissues from fish fed with contaminated feed, due to the carry-over to muscle portions. Therefore, the objective of this study was to evaluate the occurrence of 15 mycotoxins in processed fish products, which are commonly consumed, such as smoked salmon and trout, different types of sushi, and gula substitutes. A QuEChERS method was employed to perform the mycotoxin extraction from fish samples. For mycotoxin identification and quantitation, the selected technique was the liquid chromatography-tandem mass spectrometry linear ion trap (LC-MS/MS-LIT). Smoked fish and sushi samples results were negative regarding the presence of all 15 mycotoxins studied. In contrast, small amounts of fusarenon-X and enniatin B were found in gula substitute samples.

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