Characterization of Bovine Serum Albumin and (−)-Epigallocatechin Gallate/3,4-O-Dicaffeoylquinic Acid/Tannic Acid Layer by Layer Assembled Microcapsule for Protecting Immunoglobulin G in Stomach Digestion and Release in Small Intestinal Tract

2018 ◽  
Vol 66 (42) ◽  
pp. 11141-11150 ◽  
Author(s):  
Chunxu Chen ◽  
Guijie Chen ◽  
Peng Wan ◽  
Dan Chen ◽  
Tao Zhu ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Immunoglobulin G ◽  
Bovine Serum ◽  
Intestinal Tract ◽  
Epigallocatechin Gallate ◽  
Layer By Layer ◽  
Dicaffeoylquinic Acid ◽  
Small Intestinal

Characterization of binding interaction of triclosan and bovine serum albumin

2019 ◽  
Vol 55 (3) ◽  
pp. 318-325
Author(s):  
Xiaofang Wang ◽  
Luyi Zou ◽  
Chenyu Mi ◽  
Hongyan Yu ◽  
Mengxue Dong ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Binding Interaction

Characterization of bovine serum albumin hydrolysates prepared by subcritical water processing

2019 ◽  
Vol 278 ◽  
pp. 203-207 ◽  
Author(s):  
Bo-Bae Koh ◽  
Eun-Jung Lee ◽  
Karna Ramachandraiah ◽  
Geun-Pyo Hong
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Subcritical Water

Production and Characterization of Antibody Against 3′-OH-T-2 Toxin

1986 ◽  
Vol 49 (4) ◽  
pp. 267-271 ◽  
Author(s):  
RU-DONG WEI ◽  
WILLIAM BISCHOFF ◽  
FUN SUN CHU
Keyword(s):  
Detection Limit ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Cross Reactivity ◽  
Toxin A

Antibody raised against T-2 toxin cross-reacted poorly with 3′-OH-T-2 toxin. A new immunogen was prepared by conjugation of hemisuccinate (HS) of 3′-OH-T-2 toxin to bovine serum albumin (BSA). Antibodies against 3′-OH-T-2 toxin were demonstrated by a radioimmunoassay 10 wk after immunization of rabbits with this new immunogen using tritiated 3′-OH-T-2 toxin as the testing ligand. Highest titers (1:6,000) were obtained 17 wk after immunization and two booster injections. The antibodies had good cross-reactivity with T-2 toxin, acetyl-T-2 toxin and 3′-OH-acetyl-T-2 toxin. The relative cross-reactivity of this antibody with 3′-OH-T-2, acetyl-T-2, T-2, 3′-OHacetyl-T-2, 3′-OH-T-2-HS, T-2 isomer, HT-2 and 3′-OH-HT-2 was 1, 3, 4, 5, 15, 30, 45 and 175, respectively. No crossreaction was found when 3′-OH-T-2 triol, T-2-triol, T-2-tetraol, DAS and DON at a concentration of 1 μg per assay was tested. The detection limit for 3′-OH-T-2 toxin by the RIA was about 0.1 ng per assay.

Transport of bovine serum albumin across rat jejunum: role of the enteric nervous system

1994 ◽  
Vol 266 (2) ◽  
pp. G186-G193 ◽  
Author(s):  
M. H. Kimm ◽  
G. H. Curtis ◽  
J. A. Hardin ◽  
D. G. Gall
Keyword(s):  
Nervous System ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Enteric Nervous System ◽  
Intestinal Mucosa ◽  
Bovine Serum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Small Intestinal Mucosa ◽  
Rat Jejunum ◽  
Small Intestinal

To assess the mechanisms for movement of antigenically intact macromolecules across small intestinal mucosa, transport kinetics of bovine serum albumin (BSA) uptake and the effect of neural and metabolic inhibition were examined in stripped short-circuited rat jejunum. The mucosa was exposed to BSA, and, after a 50-min equilibration, mucosal-to-serosal movement of immunologically intact BSA was determined by enzyme-linked immunosorbent assay and total BSA by radiolabeled 125I-BSA. Intact BSA uptake demonstrated saturable kinetics. Immunologically intact BSA crossed the intestinal mucosa as 4.5% of total 125I-BSA flux. Colchicine and 4 degrees C significantly reduced uptake of immunologically intact BSA. NaF significantly reduced uptake of immunologically intact BSA and 125I-BSA. Treatment with tetrodotoxin significantly reduced intact BSA uptake, but did not significantly alter total BSA uptake. The muscarinic cholinoceptor antagonist atropine also significantly inhibited transport of intact BSA, whereas the nicotinic cholinoceptor antagonist hexamethonium had no effect. These findings indicate that transport of intact macromolecules across small intestinal mucosa is a saturable energy-dependent process that utilizes the microtubular network and is regulated by the enteric nervous system primarily through cholinergic nerves acting on muscarinic receptors.

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