An Approach of Utilizing Water-Soluble Carbohydrates in Lignocellulose Feedstock for Promotion of Cellulosicl-Lactic Acid Production

2018 ◽  
Vol 66 (39) ◽  
pp. 10225-10232 ◽  
Author(s):  
Xushen Han ◽  
Feng Hong ◽  
Gang Liu ◽  
Jie Bao
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Water Soluble Carbohydrates ◽  
Lignocellulose Feedstock

scholarly journals Clarifying Key Cellulase Component Cooperated With Lactic Acid Bacteria for Alfalfa Lignocellulose Degradation to Improve Lactic Acid Production

2021 ◽  
Author(s):  
Junfeng Li ◽  
Qifeng Wu ◽  
Zong Ceng ◽  
Aili Wu ◽  
Zhongyong Huang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Dry Matter ◽  
Simultaneous Saccharification And Fermentation ◽  
Lactic Acid Production ◽  
Water Soluble ◽  
Lignocellulose Degradation ◽  
Soluble Carbohydrates ◽  
Limiting Factor ◽  
Simultaneous Saccharification

Abstract Clarifying key cellulase component that played synergistic roles with lactic acid bacteria (LAB) in fermenting alfalfa lignocellulose into lactic acid (LA) is valuable in low-temperature seasons. Last cut and low dry matter (DM) alfalfa was ensiled by 9 treatments, combinations of cellulase component genes engineered Lactoc. lactis subsp. lactis MG1363 strains (HT2, HT3, HT4, HT5, E1C1, E1B1, and C1B1, separately containing bgl1, cbh2, and egl3 gene were mixed at 1:1:1, 2:1:1, 1:2:1, 1:1:2, 1:1:0, 1:0:1, and 0:1:1), cellulase (EN), and a combination of Lactobacillus plantarum and cellulase (LPEN), and without treatments, as the control, with 4 replicates each. After anaerobic preservation in a silo from late fall through winter (3-20℃) for 140 d, the ensiled alfalfa was sampled and analysed. EN degraded lignocellulose best but the pH was the key limiting factor for lignocellulose saccharification of commercial EN in the simultaneous saccharification and fermentation of LPEN. The optimal combination HT4 caused the fewest disaccharide (1.02 g/kg DM) and the highest conversion of water-soluble carbohydrates (WSC) to LA (170%) and increased LA content to 80.0 g/kg DM maximally since cellobiohydrolase better cooperated with Lactoc. lactis host to ferment lignocellulose into LA than endoglucanase and β-glucosidase. Therefore, strong LA production was approached in HT4 by clarifying key cellulase component played synergistic roles with Lactoc. lactis host. This study could benefit the development of LA production in fermenting lignocellulosic biomass.

scholarly journals Enhancing the co-ensiling performance of corn stover and cabbage waste via the addition of cellulase

BioResources ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. 6342-6362
Author(s):  
Haiwei Ren ◽  
Li Wang ◽  
Yanan Sun ◽  
Quanlin Zhao ◽  
Yongming Sun ◽  
...  
Keyword(s):  
Biological Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Corn Stover ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Water Soluble Carbohydrates ◽  
Fermentation Quality ◽  
Silage Quality

Effects of cellulase addition were assessed relative to the co-ensiling performance of air-dried corn stover (DCS) and cabbage waste (CW). The DCS and CW mixtures were co-ensiled with 0 to 0.3% of cellulase addition, and changes in composition, intermediates, and biological activity were characterized. The results showed that the addition of cellulase enhanced the decomposition of cellulose and hemicellulose by 2.51 to 6.93% and 3.41%, based on different dosages and compared with the control. Thus, the content of water-soluble carbohydrates increased. The acid content also increased from 5.8% for the control to the range 5.16 to 8.51% for the samples containing cellulase. Moreover, there was a shift from homolactic to heterolactic fermentation with prolonged ensiling time, coupled with the dominant lactic acid bacteria shifting from Paralactobacillus and Lactobacillus to more of Lactobacillus. Thus, the addition of cellulase improved the relative abundance of Lactobacillus. An assessment of fermentation quality, therefore, suggested that cellulase addition can improve the silage quality of DCS/CW during co-ensiling.

scholarly journals Influence of inoculation with Lactobacillus on fermentation, production of 1,2-propanediol and 1-propanol as well as Maize silage aerobic stability

2020 ◽  
Vol 15 (1) ◽  
pp. 373-378
Author(s):  
Marek Selwet
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Short Chain Fatty Acids ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Maize Silage ◽  
Aerobic Stability ◽  
Water Soluble Carbohydrates ◽  
L1 And L2

AbstractThe aim of this study is to determine the influence of a commercial bacterial inoculant (L1) and a preparation (L2) containing three Lactobacillus strains capable of producing 1,2-propanediol and short-chain fatty acids on maize silage aerobic stability improvement. The research showed that during 90-day ensilage, the applied preparations significantly reduced the content of DM, water-soluble carbohydrates (WSCs), pH and DM recovery (P < 0.05). The concentration of lactic acid (LA), acetic acid (AA) and propionic acid (PA) in the inoculated samples increased significantly (P < 0.05). 1,2-Propandiol and 1-propanol were not found in control silages (without additives). The addition of L1 and L2 significantly (P < 0.05) increased the concentration of these substances. The L1 and L2 mixtures significantly extended (P < 0.05) the silage aerobic stability.

scholarly journals Protective effect of titanium tetrafluoride and silver diamine fluoride on radiation-induced dentin caries in vitro

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Beatriz Martines de Souza ◽  
Mayara Souza Silva ◽  
Aline Silva Braga ◽  
Patrícia Sanches Kerges Bueno ◽  
Paulo Sergio da Silva Santos ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Protective Effect ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Negative Control ◽  
Titanium Tetrafluoride ◽  
Silver Diamine Fluoride ◽  
Dentin Caries ◽  
Radiation Induced

AbstractThis in vitro study evaluated the protective effect of titanium tetrafluoride (TiF4) varnish and silver diamine fluoride (SDF) solution on the radiation-induced dentin caries. Bovine root dentin samples were irradiated (70 Gy) and treated as follows: (6 h): 4% TiF4 varnish; 5.42% NaF varnish; 30% SDF solution; placebo varnish; or untreated (negative control). Microcosm biofilm was produced from human dental biofilm (from patients with head-neck cancer) mixed with McBain saliva for the first 8 h. After 16 h and from day 2 to day 5, McBain saliva (0.2% sucrose) was replaced daily (37 °C, 5% CO2) (biological triplicate). Demineralization was quantified by transverse microradiography (TMR), while biofilm was analyzed by using viability, colony-forming units (CFU) counting and lactic acid production assays. The data were statistically analyzed by ANOVA (p < 0.05). TiF4 and SDF were able to reduce mineral loss compared to placebo and the negative control. TiF4 and SDF significantly reduced the biofilm viability compared to negative control. TiF4 significantly reduced the CFU count of total microorganism, while only SDF affected total streptococci and mutans streptococci counts. The varnishes induced a reduction in lactic acid production compared to the negative control. TiF4 and SDF may be good alternatives to control the development of radiation-induced dentin caries.

scholarly journals Lactic acid production ability of Lactobacillus sp. from four tropical fruits using their by-products as carbon source

Heliyon ◽  
2021 ◽  
Vol 7 (5) ◽  
pp. e07079
Author(s):  
Joel Romial Ngouénam ◽  
Chancel Hector Momo Kenfack ◽  
Edith Marius Foko Kouam ◽  
Pierre Marie Kaktcham ◽  
Rukesh Maharjan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Carbon Source ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Tropical Fruits ◽  
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