Comparative Site-Specific N-Glycosylation Analysis of Lactoperoxidase from Buffalo and Goat Milk Using RP-UHPLC–MS/MS Reveals a Distinct Glycan Pattern

2018 ◽  
Vol 66 (43) ◽  
pp. 11492-11499 ◽  
Author(s):  
Gnanesh Kumar B S ◽  
Prasad Mohan Reddy ◽  
Sanjay Kottekad
Keyword(s):  
Goat Milk ◽  
Site Specific ◽  
Glycan Pattern

Large-cluster and combined fluorescent and gold immunoprobes

1996 ◽  
Vol 54 ◽  
pp. 892-893
Author(s):  
Richard D. Powell ◽  
James F. Hainfeld ◽  
Carol M. R. Halsey ◽  
David L. Spector ◽  
Shelley Kaurin ◽  
...  
Keyword(s):  
Metal Cluster ◽  
Sulfonyl Chloride ◽  
Gel Filtration ◽  
Cross Linking ◽  
Site Specific ◽  
Fab Fragments ◽  
Cluster Label ◽  
Covalently Linked ◽  
Texas Red ◽  
Fold Excess

Two new types of covalently linked, site-specific immunoprobes have been prepared using metal cluster labels, and used to stain components of cells. Combined fluorescein and 1.4 nm “Nanogold” labels were prepared by using the fluorescein-conjugated tris (aryl) phosphine ligand and the amino-substituted ligand in the synthesis of the Nanogold cluster. This cluster label was activated by reaction with a 60-fold excess of (sulfo-Succinimidyl-4-N-maleiniido-cyclohexane-l-carboxylate (sulfo-SMCC) at pH 7.5, separated from excess cross-linking reagent by gel filtration, and mixed in ten-fold excess with Goat Fab’ fragments against mouse IgG (obtained by reduction of F(ab’)2 fragments with 50 mM mercaptoethylamine hydrochloride). Labeled Fab’ fragments were isolated by gel filtration HPLC (Superose-12, Pharmacia). A combined Nanogold and Texas Red label was also prepared, using a Nanogold cluster derivatized with both and its protected analog: the cluster was reacted with an eight-fold excess of Texas Red sulfonyl chloride at pH 9.0, separated from excess Texas Red by gel filtration, then deprotected with HC1 in methanol to yield the amino-substituted label.

The challenge of detecting modifications on proteins

2020 ◽  
Vol 64 (1) ◽  
pp. 135-153 ◽  
Author(s):  
Lauren Elizabeth Smith ◽  
Adelina Rogowska-Wrzesinska
Keyword(s):  
Mass Spectrometry ◽  
Protein Function ◽  
Chemical Properties ◽  
Lysine Acetylation ◽  
Mass Determination ◽  
Post Translational Modifications ◽  
Site Specific ◽  
The Common

Abstract Post-translational modifications (PTMs) are integral to the regulation of protein function, characterising their role in this process is vital to understanding how cells work in both healthy and diseased states. Mass spectrometry (MS) facilitates the mass determination and sequencing of peptides, and thereby also the detection of site-specific PTMs. However, numerous challenges in this field continue to persist. The diverse chemical properties, low abundance, labile nature and instability of many PTMs, in combination with the more practical issues of compatibility with MS and bioinformatics challenges, contribute to the arduous nature of their analysis. In this review, we present an overview of the established MS-based approaches for analysing PTMs and the common complications associated with their investigation, including examples of specific challenges focusing on phosphorylation, lysine acetylation and redox modifications.

MEMORY EFFECTS IN THE FRAGMENTATION OF MOLECULES FOLLOWING SITE-SPECIFIC CORE EXCITATION

1987 ◽  
Vol 48 (C9) ◽  
pp. C9-741-C9-744 ◽  
Author(s):  
W. HABENICHT ◽  
L. A. CHEWTER ◽  
M. SANDER ◽  
K. MÜLLER-DETHLEFS ◽  
E. W. SCHLAG
Keyword(s):  
Memory Effects ◽  
Core Excitation ◽  
Site Specific

Sonic Movements – Spatial Reflexivity

Somatechnics ◽  
2014 ◽  
Vol 4 (1) ◽  
pp. 133-148
Author(s):  
Johanna Hällsten
Keyword(s):  
Alarm System ◽  
Edo Period ◽  
Public Places ◽  
Ongoing Research ◽  
Site Specific ◽  
Rural Environments ◽  
The World ◽  
Architectural Structures ◽  
Sonic Environment ◽  
Sonic Structures

This article aims to investigate the creation of space and sound in artistic and architectural fields, with particular emphasis on the notions of interval and duration in the production and experience of soundscapes. The discussion arises out of an ongoing research project concerning sonic structures in public places, in which Japanese uguisubari ([Formula: see text]) – ‘nightingale flooring’, an alarm system from the Edo period) plays a key role in developing new kinds of site-specific and location-responsive sonic architectural structures for urban and rural environments. This paper takes uguisubari as its frame for investigating and evaluating how sounds create a space (however temporary), and how that sound in turn is created through movement. It thus seeks to unpick aspects of the reciprocal and performative act in which participant and the space engage through movement, whilst creating a sonic environment that permeates, defines and composes the boundaries of this space. The article will develop a framework for these kinds of works through a discussion on walking, movement, soundscape and somatechnical aspects of our experience of the world, drawing upon the work of Merleau-Ponty, Bergson and the Japanese concept of Ma (space-time).

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