Reference Gene Selection and Prednisolone Target Gene Expression in Adipose Tissues of Friesian Cattle

2017 ◽  
Vol 65 (50) ◽  
pp. 11140-11145 ◽  
Author(s):  
Sara Divari ◽  
Enrica Berio ◽  
Bartolomeo Biolatti ◽  
Francesca Tiziana Cannizzo
Keyword(s):  
Gene Expression ◽  
Reference Gene ◽  
Target Gene ◽  
Gene Selection ◽  
Adipose Tissues ◽  
Target Gene Expression ◽  
Reference Gene Selection ◽  
Friesian Cattle

scholarly journals Current Practices for Reference Gene Selection in RT-qPCR of Aspergillus: Outlook and Recommendations for the Future

Genes ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 960
Author(s):  
Meagan Archer ◽  
Jianping Xu
Keyword(s):  
Gene Expression ◽  
Reference Gene ◽  
Reference Genes ◽  
Gene Selection ◽  
Quantitative Polymerase Chain Reaction ◽  
Specific Method ◽  
Experimental Conditions ◽  
Reference Gene Selection ◽  
Physiological Processes ◽  
The Stability

Aspergillus is a genus of filamentous fungi with vast geographic and ecological distributions. Species within this genus are clinically, agriculturally and biotechnologically relevant, leading to increasing interest in elucidating gene expression dynamics of key metabolic and physiological processes. Reverse-transcription quantitative Polymerase Chain Reaction (RT-qPCR) is a sensitive and specific method of quantifying gene expression. A crucial step for comparing RT-qPCR results between strains and experimental conditions is normalisation to experimentally validated reference gene(s). In this review, we provide a critical analysis of current reference gene selection and validation practices for RT-qPCR gene expression analyses of Aspergillus. Of 90 primary research articles obtained through our PubMed query, 17 experimentally validated the reference gene(s) used. Twenty reference genes were used across the 90 studies, with beta-tubulin being the most used reference gene, followed by actin, 18S rRNA and glyceraldehyde 3-phosphate dehydrogenase. Sixteen of the 90 studies used multiple reference genes for normalisation. Failing to experimentally validate the stability of reference genes can lead to conflicting results, as was the case for four studies. Overall, our review highlights the need to experimentally validate reference genes in RT-qPCR studies of Aspergillus.

scholarly journals Validation of internal reference genes for relative quantitation studies of gene expression in human laryngeal cancer

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2763 ◽  
Author(s):  
Xiaofeng Wang ◽  
Jinting He ◽  
Wei Wang ◽  
Ming Ren ◽  
Sujie Gao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reference Gene ◽  
Laryngeal Cancer ◽  
Reference Genes ◽  
Target Gene ◽  
Cancer Tissue ◽  
Internal Reference ◽  
Target Gene Expression ◽  
Internal Reference Gene ◽  
Relative Quantitation

BackgroundThe aim of this study was to determine the expression stabilities of 12 common internal reference genes for the relative quantitation analysis of target gene expression performed by reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) in human laryngeal cancer.MethodsHep-2 cells and 14 laryngeal cancer tissue samples were investigated. The expression characteristics of 12 internal reference gene candidates (18S rRNA, GAPDH, ACTB, HPRT1, RPL29, HMBS, PPIA, ALAS1, TBP, PUM1, GUSB, and B2M) were assessed by RT-qPCR. The data were analyzed by three commonly used software programs: geNorm, NormFinder, and BestKeeper.ResultsThe use of the combination of four internal reference genes was more appropriate than the use of a single internal reference gene. The optimal combination was PPIA + GUSB + RPL29 + HPRT1 for both the cell line and tissues; while the most appropriate combination was GUSB + RPL29 + HPRT1 + HMBS for the tissues.ConclusionsOur recommended internal reference genes may improve the accuracy of relative quantitation analysis of target gene expression performed by the RT-qPCR method in further gene expression research on laryngeal tumors.

Reference Gene Selection inPhenacoccus solenopsisTinsley (Hemiptera: Pseudococcidae) and Their Normalization Impact on Gene Expression in RNAi Studies

2018 ◽  
Vol 112 (1) ◽  
pp. 371-381 ◽  
Author(s):  
Satnam Singh ◽  
Suneet Pandher ◽  
Mridula Gupta ◽  
Gurmeet Kaur ◽  
Pankaj Rathore
Keyword(s):  
Gene Expression ◽  
Reference Gene ◽  
Gene Selection ◽  
Reference Gene Selection

scholarly journals Reference Gene Selection for Gene Expression Analysis of Oocytes Collected from Dairy Cattle and Buffaloes during Winter and Summer

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e93287 ◽  
Author(s):  
Carolina Habermann Macabelli ◽  
Roberta Machado Ferreira ◽  
Lindsay Unno Gimenes ◽  
Nelcio Antonio Tonizza de Carvalho ◽  
Júlia Gleyci Soares ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dairy Cattle ◽  
Reference Gene ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Gene Selection ◽  
Reference Gene Selection ◽  
Selection For
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