Isobaric Tags for Relative and Absolute Quantification (iTRAQ)-Based Untargeted Quantitative Proteomic Approach To Identify Change of the Plasma Proteins by Salbutamol Abuse in Beef Cattle

2017 ◽  
Vol 66 (1) ◽  
pp. 378-386 ◽  
Author(s):  
Kai Zhang ◽  
Chaohua Tang ◽  
Xiaowei Liang ◽  
Qingyu Zhao ◽  
Junmin Zhang
Keyword(s):  
Beef Cattle ◽  
Plasma Proteins ◽  
Absolute Quantification ◽  
Proteomic Approach ◽  
Isobaric Tags

scholarly journals iTRAQ-based comparative proteomic analysis in mature leaves of Ethiopian mustard (Brassica carinata) provides insights into the whole-proteomic profiles and the role of anthocyanin biosynthesis in leaf color diversity

2019 ◽  
Author(s):  
Tianya Wang ◽  
Shaomin Guo ◽  
Kunjiang Yu ◽  
Jun Zou ◽  
Yingfen Jiang ◽  
...  
Keyword(s):  
Bioinformatics Analysis ◽  
Anthocyanin Biosynthesis ◽  
Absolute Quantification ◽  
Biological Functions ◽  
Leaf Color ◽  
Mature Leaves ◽  
Ethiopian Mustard ◽  
Proteomic Approach ◽  
Isobaric Tags

Abstract Background Anthocyanins are the main pigments in leaves, flowers and fruits, performing diverse biological functions in plants and exhibiting benefits for human health. The purple-leaved accession of B. carinata was used to study the biosynthetic mechanism of anthocyanins in this study. To elucidate the mechanisms of anthocyanin accumulation in the purple-leaved line, we employed a proteomic approach based on an isobaric tags for relative and absolute quantification (iTRAQ)-based technique to investigate the protein expression profile of the purple-leaved line of B. carinata in comparison with that of the green-leaved line.Results In total, 4,631 proteins were identified, of which 285 exhibited significant changes in abundance between BC-G01 and BC-P01. Of the 285 DEPs, 175 are upregulated and 110 are downregulated in the leaves of the purple-leaved line compared with the leaves of the green-leaved line. Bioinformatics analysis indicated that anthocyanin biosynthesis was the most significantly elevated metabolic process, containing three DEPs corresponding to two genes UGT75C1 and UGT79B1 . UGT75C1 and UGT79B1 , encoding anthocyanin 3-O-glucoside: 2’’-O-xylosyltransferase and anthocyanin 5-O-glucosyltransferase, respectively, play an important role in the biosynthesis of anthocyanins.Conclusions The exact functions of these proteins remain to be examined. Nevertheless, our study paves the way for understanding the genetic regulatory mechanisms of anthocyanin biosynthesis and accumulation in B. carinata .

scholarly journals Quantitative Proteomics by iTRAQ-PRM based Reveals the New Characterization for Gout.

2021 ◽  
Author(s):  
Guangqi Chen ◽  
Jiafen Cheng ◽  
Hanjie Yu ◽  
Xiao Huang ◽  
Hui Bao ◽  
...  
Keyword(s):  
Plasma Proteins ◽  
Complex Form ◽  
Absolute Quantification ◽  
Acute Gout ◽  
Histone H2a ◽  
Histone H2b ◽  
Patient Will ◽  
Novel Biomarkers ◽  
Isobaric Tags ◽  
Complement Component C8

Abstract BackgroundGout is a common and complex form of immunoreactive arthritis based on hyperuricemia, while the symptoms would turn to remission or even got worse. So, it is hard to early identify whether an asymptomatic hyperuricemia (AHU) patient will be susceptible to get acute gout attack and it is also hard to predict the process of gout remission to flare. Here, we report that the plasma proteins profile can distinguish among acute gout (AG), remission of gout (RG), AHU patients, and healthy controls.MethodsWe established an isobaric tags for relative and absolute quantification (iTRAQ) and parallel reaction monitoring (PRM) based method to measure the plasma proteins for AG group (n=8), RG group(n=7), AHU group(n=7) and healthy controls(n=8).Resultseleven differentially expressed proteins such as Histone H2A, Histone H2B, Thrombospondin-1 (THBS1), Myeloperoxidase (MPO), Complement C2, Complement component C8 beta chain (C8B), Alpha-1-acid glycoprotein 1 (ORM1), Inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), Carbonic anhydrase 1 (CA1), Serum albumin (ALB) and Multimerin-1 (MMRN1) were identified. Histone H2A, Histone H2B and THBS1 might be the strongest influential regulator to maintain the balance and stability of the gout process. The complement and coagulation cascades is one of the main functional pathways in the mechanism of gout process.ConclusionsHistone H2A, Histone H2B and THBS1 may be novel biomarkers in discriminating gout attack from AHU or RG, providing new theoretical insights for the prognosis, treatment, and management of gout process.Trial registrationThis study is not a clinical trial.

scholarly journals Quantitative proteomics by iTRAQ-PRM based reveals the new characterization for gout

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Guangqi Chen ◽  
Jiafen Cheng ◽  
Hanjie Yu ◽  
Xiao Huang ◽  
Hui Bao ◽  
...  
Keyword(s):  
Plasma Proteins ◽  
Complex Form ◽  
Absolute Quantification ◽  
Acute Gout ◽  
Potential Candidate ◽  
Healthy Controls ◽  
Histone H2a ◽  
Histone H2b ◽  
Patient Will ◽  
Isobaric Tags

Abstract Background Gout is a common and complex form of immunoreactive arthritis based on hyperuricemia, while the symptoms would turn to remission or even got worse. So, it is hard to early identify whether an asymptomatic hyperuricemia (AHU) patient will be susceptible to get acute gout attack and it is also hard to predict the process of gout remission to flare. Here, we report that the plasma proteins profile can distinguish among acute gout (AG), remission of gout (RG), AHU patients, and healthy controls. Methods We established an isobaric tags for relative and absolute quantification (iTRAQ) and parallel reaction monitoring (PRM) based method to measure the plasma proteins for AG group (n = 8), RG group (n = 7), AHU group (n = 7) and healthy controls (n = 8). Results Eleven differentially expressed proteins such as Histone H2A, Histone H2B, Thrombospondin-1 (THBS1), Myeloperoxidase (MPO), Complement C2, Complement component C8 beta chain (C8B), Alpha-1-acid glycoprotein 1 (ORM1), Inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), Carbonic anhydrase 1 (CA1), Serum albumin (ALB) and Multimerin-1 (MMRN1) were identified. Histone H2A, Histone H2B and THBS1 might be the strongest influential regulator to maintain the balance and stability of the gout process. The complement and coagulation cascades is one of the main functional pathways in the mechanism of gout process. Conclusions Histone H2A, Histone H2B and THBS1 are potential candidate genes for novel biomarkers in discriminating gout attack from AHU or RG, providing new theoretical insights for the prognosis, treatment, and management of gout process. Trial registration This study is not a clinical trial.

scholarly journals The isobaric tags for relative and absolute quantification-based quantitative proteomics of fresh tissue-derived secretome in hepatocellular carcinoma

2021 ◽  
Author(s):  
Hai-Tao Jiang ◽  
Guo-Sheng Gao ◽  
Feng Ren ◽  
Yun-Jie Chen
Keyword(s):  
Hepatocellular Carcinoma ◽  
Quantitative Proteomics ◽  
Primary Cultures ◽  
Absolute Quantification ◽  
Janus Kinase ◽  
Cellular Growth ◽  
Mitogen Activated Protein Kinase ◽  
Proteomic Approach ◽  
Mapk Signalling ◽  
Isobaric Tags

IntroductionProteomics technology platforms offer an extremely useful tool for the discovery of new cancer biomarkers. Secreted proteins play important roles in signal transduction, cellular growth, proliferation, differentiation, and apoptosis. This study aimed to investigate the molecular signatures of the hepatocellular carcinoma (HCC) by quantitative proteomics using isobaric tags for relative and absolute quantification (iTRAQ) with liquid chromatography-tandem mass spectrometry (LC-MS/MS).Material and methodsIn this study, we used an iTRAQ-based quantitative proteomic approach to analyse the secretome of HCC tissues to identify plasma biomarkers. Serum-free conditioned media (CM) were collected from the primary cultures of cancerous tissues, the surrounding noncancerous tissues, and distal noncancerous tissues.ResultsA proteomic analysis of the CM proteins allowed for a total of 5214 identified proteins, of which 190 and 44 proteins were dysregulated in the HCC tissues/distal noncancerous tissues (HCC/DN group) and the adjacent noncancerous tissues/distal noncancerous tissues (AN/DN group) compared with the distal noncancerous tissues. The dysregulated proteins in the HCC/DN group were concentrated in mitogen-activated protein kinase (MAPK) signalling and Janus kinase-signal transducer and activator of the transcription (JAK-STAT) signalling, but the dysregulated proteins in the AN/DN group were more concentrated in the basal material metabolism.ConclusionsThe secretome profile alternations and signalling pathways were associated with HCC incidence and development. The dysregulated proteins in the HCC/DN group were concentrated in the MAPK signalling and JAK-STAT signalling, but the dysregulated proteins in the AN/DN group were more concentrated in the basal material metabolism.

Proteomic approach for purification of seminal plasma proteins involved in tumor proliferation

2007 ◽  
Vol 30 (12) ◽  
pp. 1979-1988 ◽  
Author(s):  
Md Imtaiyaz Hassan ◽  
Vijay Kumar ◽  
Tara Kashav ◽  
Neelima Alam ◽  
Tej P. Singh ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Tumor Proliferation ◽  
Proteomic Approach ◽  
Seminal Plasma Proteins

scholarly journals iTRAQ-Based Proteomics Analysis of Autophagy-Mediated Responses against MeJA in Laticifers of Euphorbia kansui L.

2019 ◽  
Vol 20 (15) ◽  
pp. 3770
Author(s):  
Fang ◽  
Yao ◽  
Zhang ◽  
Tian ◽  
Wang ◽  
...  
Keyword(s):  
Developmental Process ◽  
Cysteine Proteinase ◽  
Defense Responses ◽  
Absolute Quantification ◽  
Pcr Analysis ◽  
Control Group ◽  
Proteomics Analysis ◽  
Meja Treatment ◽  
Euphorbia Kansui ◽  
Isobaric Tags

Autophagy is a well-defined catabolic mechanism whereby cytoplasmic materials are engulfed into a structure termed the autophagosome. Methyl jasmonate (MeJA), a plant hormone, mediates diverse developmental process and defense responses which induce a variety of metabolites. In plants, little is known about autophagy-mediated responses against MeJA. In this study, we used high-throughput comparative proteomics to identify proteins of latex in the laticifers. The isobaric tags for relative and absolute quantification (iTRAQ) MS/MS proteomics were performed, and 298 proteins among MeJA treated groups and the control group of Euphorbia kansui were identified. It is interesting to note that 29 significant differentially expressed proteins were identified and their associations with autophagy and ROS pathway were verified for several selected proteins as follows: α-L-fucosidase, β-galactosidase, cysteine proteinase, and Cu/Zn superoxide dismutase. Quantitative real-time PCR analysis of the selected genes confirmed the fact that MeJA might enhance the expression of some genes related to autophagy. The western blotting and immunofluorescence results of ATG8 and ATG18a which are two important proteins for the formation of autophagosomes also demonstrated that MeJA could promote autophagy at the protein level. Using the electron microscope, we observed an increase in autophagosomes after MeJA treatment. These results indicated that MeJA might promote autophagy in E. kansui laticifers; and it was speculated that MeJA mediated autophagy through two possible ways: the increase of ROS induces ATG8 accumulation and then aotophagosome formation, and MeJA promotes ATG18 accumulation and then autophagosome formation. Taken together, our results provide several novel insights for understanding the mechanism between autophagy and MeJA treatment. However, the specific mechanism remains to be further studied in the future.

scholarly journals Partial Proteome Map of Campylobacter Jejuni Strain Nctc11168 by Gel-Free Proteomics Analysis

2014 ◽  
Vol 2 (4) ◽  
pp. 464-477
Author(s):  
Zilun Shi ◽  
Chris Dawson ◽  
Stephen L.W. On ◽  
Malik Altaf Hussain
Keyword(s):  
Campylobacter Jejuni ◽  
Foodborne Pathogen ◽  
Brain Heart Infusion ◽  
Cell Protein ◽  
Absolute Quantitation ◽  
Whole Cell ◽  
Itraq Analysis ◽  
Proteomic Approach ◽  
Whole Cell Protein ◽  
Isobaric Tags

A proteome map of the foodborne pathogen Campylobacter jejuni NCTC11168 was analyzed using a state-of-the-art gel-free proteomic approach for the first time. A whole cell protein extract was prepared from the C. jejuni strain NCTC11168 grown in brain heart infusion (BHI) broth at 42°C under microaerobic conditions. A gel-free technique using isobaric tags for relative and absolute quantitation (iTRAQ) was employed to create a protein expression profile of the strain. Liquid chromatography-mass spectrometry (LC-MS/MS) was used to identify the proteins. Protein functionalities were searched to classify them. A total of 235 proteins were identified in the whole cell protein fraction of C. jejuni NCTC11168 cells using iTRAQ analysis. Functional grouping of the identified proteins showed that forty percent of these proteins were associated with energy metabolism, protein synthesis and genetic information processing. iTRAQ was faster, easier and proved more sensitive than two-dimensional gel-based proteomics approaches previously applied to C. jejuni, making it an attractive tool for further studies of cellular physiological response. DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11253  Int J Appl Sci Biotechnol, Vol. 2(4): 464-477 

scholarly journals Comparative Proteomic Analysis of Dipsacus asperoides Roots from Different Habitats in China

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3605
Author(s):  
Haijun Jin ◽  
Hua Yu ◽  
Haixia Wang ◽  
Jia Zhang
Keyword(s):  
Proteomic Analysis ◽  
Absolute Quantification ◽  
Pcr Analysis ◽  
Allene Oxide ◽  
Allene Oxide Cyclase ◽  
Protein Levels ◽  
Depth Analysis ◽  
Differential Proteins ◽  
Isobaric Tags

Dipsacus asperoides is a kind of Chinese herbal medicine with beneficial health properties. To date, the quality of D. asperoides from different habitats has shown significant differences. However, the molecular differences in D. asperoides from different habitats are still unknown. The aim of this study was to investigate the differences in protein levels of D. asperoides from different habitats. Isobaric tags for relative and absolute quantification (iTRAQ) and 2DLC/MS/MS were used to detect statistically significant changes in D. asperoides from different habitats. Through proteomic analysis, a total of 2149 proteins were identified, of which 42 important differentially expressed proteins were screened. Through in-depth analysis of differential proteins, the protein metabolism energy and carbohydrate metabolism of D. asperoides from Hubei Province were strong, but their antioxidant capacity was weak. We found that three proteins, UTP-glucose-1-phosphate uridylyltransferase, allene oxide cyclase, and isopentyl diphosphate isomerase 2, may be the key proteins involved in dipsacus saponin VI synthesis. Eight proteins were found in D. asperoides in response to environmental stress from different habitats. Quantitative real-time PCR analysis confirmed the accuracy and authenticity of the proteomic analysis. The results of this study may provide the basic information for exploring the cause of differences in secondary metabolites in different habitats of D. asperoides and the protein mechanism governing differences in quality.

scholarly journals Quantitative proteomic analysis of G-protein signalling inStagonospora nodorumusing isobaric tags for relative and absolute quantification

PROTEOMICS ◽  
2010 ◽  
Vol 10 (1) ◽  
pp. 38-47 ◽  
Author(s):  
Tammy Casey ◽  
Peter S. Solomon ◽  
Scott Bringans ◽  
Kar-Chun Tan ◽  
Richard P. Oliver ◽  
...  
Keyword(s):  
G Protein ◽  
Proteomic Analysis ◽  
Absolute Quantification ◽  
Quantitative Proteomic Analysis ◽  
G Protein Signalling ◽  
Isobaric Tags
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