Study of Substituted Ester Formation in Red Wine by the Development of a New Method for Quantitative Determination and Enantiomeric Separation of Their Corresponding Acids

2017 ◽  
Vol 65 (24) ◽  
pp. 5018-5025 ◽  
Author(s):  
Georgia Lytra ◽  
Celine Franc ◽  
Margaux Cameleyre ◽  
Jean-Christophe Barbe
Keyword(s):  
Quantitative Determination ◽  
Red Wine ◽  
Enantiomeric Separation ◽  
New Method ◽  
Ester Formation

AGGLUTINATION INHIBITION REACTIONS FOR THE DETERMINATION OF GONADOTROPHINS

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S95-S112 ◽  
Author(s):  
A. H. W. M. Schuurs
Keyword(s):  
Immune System ◽  
Quantitative Determination ◽  
Haemagglutination Inhibition ◽  
Latex Agglutination ◽  
Literature Survey ◽  
New Method ◽  
Test Fluid ◽  
Main Application ◽  
Test Systems

ABSTRACT Various techniques for sensitising erythrocytes and latex particles with gonadotrophins, particularly with HCG, are described. The haemagglutination inhibition reactions are generally interpreted by means of »erythrocyte settling patterns«. By a new method of evaluating these patterns a relatively precise quantitative determination is possible. Latex agglutination inhibition reactions on slides are particularly suitable as rapid qualitative tests. In cases where the maximum attainable sensitivity of the agglutination inhibition tests is insufficient, e. g. for determining LH concentrations in urine, the hormone in the test fluid has to be concentrated or extracted. An alternative method is a modified haemagglutination inhibition test for large volumes which is applicable to unconcentrated urine. Due to non-specific inhibitions the above-mentioned tests cannot be applied to unprocessed serum. Agglutination inhibition tests with HCG are already well advanced, pregnancy diagnosis being their main application. Now that highly purified HCG is available, a satisfactory specificity for these tests can be attained. If the immune system for HCG is used for estimating LH, it has to meet additional specificity requirements. Furthermore, the measure of cross-reaction and the choice of standard merit special attention. Finally, a literature survey is given of test systems in which LH and FSH were used as antigens.

scholarly journals ON THE CALCULATION OF "TURNOVER TIME" AND "TURNOVER RATE" FROM EXPERIMENTS INVOLVING THE USE OF LABELING AGENTS

1943 ◽  
Vol 26 (3) ◽  
pp. 325-331 ◽  
Author(s):  
D. B. Zilversmit ◽  
C. Entenman ◽  
M. C. Fishler
Keyword(s):  
Quantitative Determination ◽  
Turnover Rate ◽  
Turnover Time ◽  
New Method ◽  
Animal Body

1. A new method for the determination of an immediate precursor of a substance occurring in the animal body is presented. 2. Calculations on the quantitative determination of the rate of turnover of a substance and their application to experiments involving the use of labeling agents are given. These calculations take into account loss of the isotopic substance by way of breakdown or transport.

Optimization of an Aqueous Two-Phase System for the Determination of Trace Ethyl Carbamate in Red Wine

2019 ◽  
Vol 82 (8) ◽  
pp. 1377-1383 ◽  
Author(s):  
LIJUAN MA ◽  
WENZHE TONG ◽  
LIPING DU ◽  
SHIYONG HUANG ◽  
JINYAN WEI ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Red Wine ◽  
Ethyl Carbamate ◽  
Phase System ◽  
Extraction Temperature ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Relative Standard ◽  
Standard Deviations

ABSTRACT In this study, a novel method using gas chromatography–mass spectrometry coupled with ethanol and K2HPO4 aqueous two-phase system (ATPS) was established for the quantitative determination of trace ethyl carbamate (EC) in red wine. The parameters that influence EC extraction in an aqueous two-phase system, including extraction temperature, time, pH, and ethanol concentration, were optimized. Method validation results indicated that the regression coefficient of the proposed method was 0.9979 in the linear range of 10 to 100 μg/L, and the limits of detection and quantification were 2.8 and 9.2 μg/L, respectively. Four red wine samples made from different grape varieties were processed by the proposed method for the repeatability verification, and EC concentrations were between 15.8 and 37.3 μg/L, with the relative standard deviations ranging from 3.5 to 6.6%. Results of the precision assay showed the average recovery of EC in red wine at 95.4 to 107.1%, with the relative standard deviations ranging from 1.4 to 6.2%. This method proved to be simple and reliable for quantitative determination of trace EC in red wine and would give guidance for quality monitoring of various red wines in the production process.

scholarly journals A new method for quantitative determination of protein associated with crustacean chitin.

1987 ◽  
Vol 61 (4) ◽  
pp. 437-441 ◽  
Author(s):  
Yasuyuki TAKIGUCHI ◽  
Hiroshi YAMASHITA ◽  
Kazuhiro OHKOUCHI ◽  
Kenzo SHIMAHARA
Keyword(s):  
Quantitative Determination ◽  
New Method

scholarly journals A NEW METHOD FOR THE QUANTITATIVE DETERMINATION OF PROTEIN OF BACTERIAL ORIGIN ON THE BASIS OF D-ASPARTIC ACID AND D-GLUTAMIC ACID CONTENT

2001 ◽  
Vol 30 (1) ◽  
pp. 37-52 ◽  
Author(s):  
J. Csapó ◽  
J. Schmidt ◽  
Zs. Csapó-Kiss ◽  
G. Holló ◽  
I. Holló ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Acid Content ◽  
New Method ◽  
Bacterial Origin

scholarly journals Pharmacokinetics and Bioavailability Study of Monocrotaline in Mouse Blood by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Lianguo Chen ◽  
Bin Zhang ◽  
Jinlai Liu ◽  
Zhehua Fan ◽  
Ziwei Weng ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Quantitative Determination ◽  
Ultra Performance Liquid Chromatography ◽  
New Method ◽  
Tandem Mass ◽  
Mouse Blood ◽  
Detection Range

Background and Aims. The present study aimed to develop a simple and sensitive method for quantitative determination of monocrotaline (MCT) in mouse blood employing ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI/MS/MS) using rhynchophylline as an internal standard. Methods. Proteins present in the blood samples were precipitated using acetonitrile. MCT was separated using a 1.7-μm ethylene bridged hybrid (BEH) C18 column (2.1 mm × 50 mm) with a gradient elution program and a constant flow rate of 0.4 mL/min. The LC mobile phase consisted of 10 mmol/L ammonium acetate (containing 0.1% formic acid) and acetonitrile. The total elution time was 4.0 min. The analytes were detected on a UPLC-ESI mass spectrometer in multiple reaction monitoring (MRM) mode and quantified. Results. The new method for the determination of MCT has a satisfactory linear detection range of 1-2000 ng/mL and excellent linearity (r = 0.9971). The lower limit of quantification (LLOQ) of MCT is 1.0 ng/mL. Intra- and interassay precisions of MCT were ≤13% with an accuracy from 96.2% to 106.6%. The average recovery of the new method was >75.0%, and matrix effects were between 89.0% and 94.3%. Based on the pharmacokinetics data, the bioavailability of MCT in mice was 88.3% after oral administration. Conclusions. The results suggest that the newly standardized method for quantitative determination of MCT in whole blood is fast, reliable, specific, sensitive, and suitable for pharmacokinetic studies of MCT after intravenous or intragastric administration.

A new method for the quantitative determination of zeolitic Brønsted acidity

1997 ◽  
pp. 383-384 ◽  
Author(s):  
Manfred Richter ◽  
Barbara Parlitz ◽  
Reinhard Eckelt ◽  
Rolf Fricke
Keyword(s):  
Quantitative Determination ◽  
New Method ◽  
Brønsted Acidity ◽  
Brönsted Acidity

A new method for the quantitative determination of enzymes splitting N-acetyl tyrosine ethylester

1968 ◽  
Vol 19 (3) ◽  
pp. 405-409 ◽  
Author(s):  
M. Rybák ◽  
V. Mansfeld ◽  
M. Petáková ◽  
E. Simonianová
Keyword(s):  
Quantitative Determination ◽  
New Method
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