Cooking Has Variable Effects on the Fermentability in the Large Intestine of the Fraction of Meats, Grain Legumes, and Insects That Is Resistant to Digestion in the Small Intestine in an in Vitro Model of the Pig’s Gastrointestinal Tract

2017 ◽  
Vol 65 (2) ◽  
pp. 435-444 ◽  
Author(s):  
Christine Poelaert ◽  
Xavier Despret ◽  
Marianne Sindic ◽  
Yves Beckers ◽  
Frédéric Francis ◽  
...  
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Large Intestine ◽  
In Vitro Model ◽  
Grain Legumes ◽  
Vitro Model

Survival of a probiotic-containing product using capsule-within-capsule technology in an in vitro model of the stomach and small intestine (TIM-1)

2020 ◽  
Vol 11 (4) ◽  
pp. 403-409
Author(s):  
K. Venema ◽  
J. Verhoeven ◽  
C. Beckman ◽  
D. Keller
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
In Vitro Model ◽  
Additional Benefit ◽  
Outer Capsule ◽  
Probiotic Strains ◽  
Human Adults ◽  
Similar Survival ◽  
Vitro Model

The aim of the research was to compare the survival of a blend of five probiotic strains (2 bifidobacteria and 3 lactobacilli) in a capsule within capsule (Duocap®) containing Ahiflower® oil, as compared to the strains in the powder (with or without Ahiflower oil), or the strains when present in the inner capsule only. This was tested in a validated, dynamic in vitro model of the stomach and small intestine (TIM-1), simulating human adults. Experiments were performed both in the gastric compartment of the model, as well as in the complete system (stomach + small intestine). Survival of the strains after transit through the gastric compartment in the Duocap capsule was higher by about a factor of 1.5 compared to the other 3 variables. In these gastric experiments, the Ahiflower oil did not seem to have an additional benefit, in the sense that it did not increase survival over the strains alone. After transit through the complete gastrointestinal tract survival was approximately 2-fold higher for the strains within the Duocap capsule, compared to the strains within the inner capsule or the powder. In these experiments, Ahiflower oil did have an additional benefit. The survival of the strains in the combination of powder with Ahiflower oil showed a similar survival as that of the Duocap, although in the first few hours of the experiments survival of both species lagged behind, and only caught up at the end of the test. In conclusion, the developed capsule-in-capsule technology increased the amount of viable cells in the upper gastrointestinal tract, mainly due to the presence of the polyunsaturated fatty acids contained in the outer capsule, which particularly protected the blend of probiotics in the small intestine.

scholarly journals Gastrointestinal digestion of dietary advanced glycation endproducts using an in vitro model of the gastrointestinal tract (TIM-1)

2020 ◽  
Vol 11 (7) ◽  
pp. 6297-6307 ◽  
Author(s):  
Timme van der Lugt ◽  
Koen Venema ◽  
Stefan van Leeuwen ◽  
Misha F. Vrolijk ◽  
Antoon Opperhuizen ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
In Vitro Model ◽  
Advanced Glycation Endproducts ◽  
Food Products ◽  
Advanced Glycation ◽  
Gastrointestinal Digestion ◽  
Glycation Endproducts ◽  
Vitro Model

In a sophisticated gastrointestinal model, dietary advanced glycation endproducts (dAGEs) in food products remain bound to proteins after digestion and concentrations increase.

scholarly journals Silicon dioxide nanoparticle exposure affects small intestine function in an in vitro model

2018 ◽  
Vol 12 (5) ◽  
pp. 485-508 ◽  
Author(s):  
Zhongyuan Guo ◽  
Nicole J. Martucci ◽  
Yizhong Liu ◽  
Eusoo Yoo ◽  
Elad Tako ◽  
...  
Keyword(s):  
Small Intestine ◽  
Silicon Dioxide ◽  
In Vitro Model ◽  
Nanoparticle Exposure ◽  
Vitro Model ◽  
Silicon Dioxide Nanoparticle

scholarly journals Trolox and Ascorbic Acid Reduce Direct and Indirect Oxidative Stress in the IPEC-J2 Cells, an In Vitro Model for the Porcine Gastrointestinal Tract

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0120485 ◽  
Author(s):  
Hans Vergauwen ◽  
Bart Tambuyzer ◽  
Karen Jennes ◽  
Jeroen Degroote ◽  
Wei Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Gastrointestinal Tract ◽  
In Vitro Model ◽  
Vitro Model

Influence of Bacillus subtilis C-3102 on microbiota in a dynamic in vitro model of the gastrointestinal tract simulating human conditions

2012 ◽  
Vol 3 (3) ◽  
pp. 229-236 ◽  
Author(s):  
M. Hatanaka ◽  
Y. Nakamura ◽  
A.J.H. Maathuis ◽  
K. Venema ◽  
I. Murota ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Gastrointestinal Tract ◽  
In Vitro Model ◽  
Germination Rate ◽  
Human Colon ◽  
Micro Array ◽  
Vitro Model ◽  
Oral Doses ◽  
The Impact

Survival and germination rate of Bacillus subtilis C-3102 spores were investigated in a stomach and small intestine model (TIM-1), while the impact of C-3102 cells that had passed through TIM-1 on human colon microbiota was evaluated in a model of the large intestine (TIM-2). The survival of C-3102 spores in TIM-1 was 99%; 8% of the spores had germinated. Effluent of TIM-1 was subsequently introduced into TIM-2 and a micro-array platform was employed to assess changes in the microbiota composition. The effluent, which contained germinated C-3102 cells, increased some Bifidobacterium species and decreased some Clostridium groups. These changes were greater compared to those obtained by adding C-3102 spores directly to TIM-2. The present study suggests that oral doses of B. subtilis C-3102 spores have the potential to modulate the human colon microbiota. This effect may be caused by germination of the spores in the gastrointestinal tract.

Differentiated CaCo-2 cells as an in-vitro model to evaluate de-novo apolipoprotein A-I production in the small intestine

2009 ◽  
Vol 21 (6) ◽  
pp. 642-649 ◽  
Author(s):  
Stefan P.J. Dullens ◽  
Ronald P. Mensink ◽  
Edwin C.M. Mariman ◽  
Jogchum Plat
Keyword(s):  
Small Intestine ◽  
In Vitro Model ◽  
De Novo ◽  
Apolipoprotein A ◽  
Vitro Model
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