scholarly journals Identification of Pyrogallol in the Ethyl Acetate-Soluble Part of Coffee as the Main Contributor to Its Xanthine Oxidase Inhibitory Activity

2016 ◽  
Vol 64 (41) ◽  
pp. 7743-7749 ◽  
Author(s):  
Sari Honda ◽  
Toshiya Masuda
Keyword(s):  
Ethyl Acetate ◽  
Xanthine Oxidase ◽  
Inhibitory Activity ◽  
Main Contributor ◽  
Soluble Part

scholarly journals Phytochemical Composition, Antioxidant Activity, and Enzyme Inhibitory Activities (α-Glucosidase, Xanthine Oxidase, and Acetylcholinesterase) of Musella lasiocarpa

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4472
Author(s):  
Rurui Li ◽  
Yuerong Ru ◽  
Zhenxing Wang ◽  
Xiahong He ◽  
Kin-Weng Kong ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Xanthine Oxidase ◽  
Inhibitory Activity ◽  
Methanol Extract ◽  
Total Phenolic ◽  
Aqueous Fraction ◽  
Antioxidant Power ◽  
Etoac Fraction ◽  
Inhibitory Activities ◽  
Crude Methanol Extract

In this study, we aimed to investigate the chemical components and biological activities of Musella lasiocarpa, a special flower that is edible and has functional properties. The crude methanol extract and its four fractions (petroleum ether, ethyl acetate, n-butanol, and aqueous fractions) were tested for their total antioxidant capacity, followed by their α-glucosidase, acetylcholinesterase, and xanthine oxidase inhibitory activities. Among the samples, the highest total phenolic and total flavonoid contents were found in the ethyl acetate (EtOAc) fraction (224.99 mg GAE/g DE) and crude methanol extract (187.81 mg QE/g DE), respectively. The EtOAc fraction of Musella lasiocarpa exhibited the strongest DPPH· scavenging ability, ABTS·+ scavenging ability, and α-glucosidase inhibitory activity with the IC50 values of 22.17, 12.10, and 125.66 μg/mL, respectively. The EtOAc fraction also showed the strongest ferric reducing antioxidant power (1513.89 mg FeSO4/g DE) and oxygen radical absorbance capacity ability (524.11 mg Trolox/g DE), which were higher than those of the control BHT. In contrast, the aqueous fraction demonstrated the highest acetylcholinesterase inhibitory activity (IC50 = 10.11 μg/mL), and the best xanthine oxidase inhibitory ability (IC50 = 5.23 μg/mL) was observed from the crude methanol extract as compared with allopurinol (24.85 μg/mL). The HPLC-MS/MS and GC-MS analyses further revealed an impressive arsenal of compounds, including phenolic acids, fatty acids, esters, terpenoids, and flavonoids, in the most biologically active EtOAc fraction. Taken together, this is the first report indicating the potential of Musella lasiocarpa as an excellent natural source of antioxidants with possible therapeutic, nutraceutical, and functional food applications.

scholarly journals Flavonoid isolation and identification of mother-in-law’s tongue leaves (sansevieria trifasciata) and the inhibitory activities to xanthine oxidase enzyme

2018 ◽  
Vol 67 ◽  
pp. 03011 ◽  
Author(s):  
Abdullah ◽  
Angelina ◽  
Mauhibah Yumna ◽  
Rita Arbianti ◽  
Tania Surya Utami ◽  
...  
Keyword(s):  
Uric Acid ◽  
Ethyl Acetate ◽  
Xanthine Oxidase ◽  
Crude Extract ◽  
Inhibitory Activity ◽  
Enzyme Inhibitor ◽  
The Body ◽  
Isolation And Identification ◽  
Oxidase Enzyme ◽  
Antioxidant Agent

Hyperuricemia is a disease that is characterized by high uric acid levels, in which the number of victim increase year by year in the worldwide. Flavonoid is an active compound with inhibitory activity towards Xanthine Oxidase Enzyme which is a compound that plays a role in the formation of uric acid in the body. Sansevieria trifasciata is an ornamental plant which is also useful as a source of antibacterial and antioxidant agent. Studies of S. trifasciata as Xanthine Oxidase Enzyme inhibitor have not been reported. This research isolate flavonoid compounds using open column chromatography from crude extract of S. trifasciata leaves that extracted by sonication method. There are six eluent used to isolate flavonoid which are methanol : ethyl acetate, chloroform : ethyl acetate, chloroform : ethyl acetate : methanol. Wilstater test is used to select the fraction that rich of flavonoid. The best result from isolation step that contains flavonoid is assessed the inhibitory activity of xanthine oxidase. It is analyzed qualitative using Liquid Chromatography Mass Spectrometry (LCMS). The inhibition percentage showed that fraction 3 was potential to inhibit XO by 85.48 %. LC-MS chromatogram can show that crude extract and positive fraction of isolation were containing falvonoid.

scholarly journals ANTIHYPERURICEMIC AND XANTHINE OXIDASE INHIBITORY ACTIVITIES OF FRACTIONS FROM ETHANOLIC LEAVES EXTRACT OF STELECHOCARPUS BURAHOL

2016 ◽  
Vol 9 (6) ◽  
pp. 255
Author(s):  
Titik Sunarni
Keyword(s):  
Uric Acid ◽  
Ethyl Acetate ◽  
Xanthine Oxidase ◽  
Inhibitory Activity ◽  
Extraction Method ◽  
Acid Level ◽  
Liquid Extraction ◽  
Liquid Liquid Extraction ◽  
Activity Test ◽  
Inhibitory Activities

Objectives: The aims of the research were to evaluate antihyperuricemic and xanthine oxidase (XO) inhibitory activity of fractions from ethanolic leaves extract of Stelechocarpus burahol. Methods: S. burahol leaves powder was extracted in ethanol by maceration method, then extract was fractionated by liquid-liquid extraction method using n-hexane and ethyl acetate. Hyperuricemic rat model was induced by administered potassium oxonate intraperitoneally. The activity of these fractions on XO inhibitory was determined by measuring the uric acid formation using UV-Vis spectrophotometry method after incubating with XO.   Results: The n-hexane and ethyl acetate fractions significantly (p < 0.05) reduce the uric level by 32.0 and 28.0% respectively compared to uric acid level of hyperuricemic group at one hour after drug administration (p < 0.05). On XO inhibitory activity test, only n-hexane fraction exhibited XO inhibitory activity with IC50 541.76 µg/ml. Conclusion: The n-hexane and ethyl acetate fractions from ethanolic leaves extract of S.burahol had antihiperuricemic activity, but less inhibitor effect on XO activity. Keywords: Stelechocarpus burahol, liquid-liquid extraction, antihyperuricemic, xanthine oxidase inhibitory activity 

Screening of Hungarian mushrooms for xanthine-oxidase inhibitory activity

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
A Ványolós ◽  
O Orbán-Gyapai ◽  
T Támadi ◽  
J Hohmann
Keyword(s):  
Xanthine Oxidase ◽  
Inhibitory Activity

Polyphenols from Cyclopia genistoides and their xanthine oxidase inhibitory activity

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
O Roza ◽  
A Martins ◽  
J Hohmann ◽  
WC Lai ◽  
FR Chang ◽  
...  
Keyword(s):  
Xanthine Oxidase ◽  
Inhibitory Activity

Verbascum sinaiticum: A Rich Source of Antioxidant Phenylethanoid Glycosides

2020 ◽  
Vol 10 (2) ◽  
pp. 158-162 ◽  
Author(s):  
Humaira Yasmeen Gondal ◽  
Roshan Zamir ◽  
Muhammad Nisar ◽  
Muhammad Iqbal Choudhary
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Aerial Part ◽  
Methanolic Extract ◽  
Water Soluble ◽  
Antioxidant Potential ◽  
Active Fraction ◽  
Phenylethanoid Glycosides ◽  
Active Constituents ◽  
Soluble Part

Background: The genus Verbascum is well documented for its antioxidant potential but Verbascum sinaiticum is comparatively less studied plant. The current study was carried out to search for antioxidant nutraceuticals from this species. Objective: To explore the antioxidant potential of Verbascum sinaiticum and to identify its active constituents. Methods: The methanolic extract of air-dried aerial part of the Verbascum sinaiticum was partitioned with hexane, chloroform and ethyl acetate. The water-soluble part of ethyl acetate afforded six phenylethanoid glycosides by repeated chromatography over Sephadex LH-20, silica gel and ODS columns. Antioxidant activity of solvent extracts and isolated constituents were evaluated by DPPH, ABTS and FRAP assays. Results: Six phenylethanoid glycosides was isolated and characterized as Verbascoside, Eukovoside, Martynoside, Jionoside D, Campneoside I and Campneoside II, from the most active fraction. Conclusion: Verbascum sinaiticum demonstrated prospective antioxidant activity. The watersoluble part of EtOAc (WSEAE) was found the most active extract whereas Verbascoside was identified as the most potent constituent. All isolated compounds exhibited significant antioxidant activity whereas their synergistic effect was found prominent in the parent fraction.

scholarly journals Evaluation of Enzyme Inhibitory Activity of Flavonoids by Polydopamine-Modified Hollow Fiber-Immobilized Xanthine Oxidase

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3931
Author(s):  
Cong-Peng Zhao ◽  
Guo-Ying Chen ◽  
Yuan Wang ◽  
Hua Chen ◽  
Jia-Wen Yu ◽  
...  
Keyword(s):  
Xanthine Oxidase ◽  
Hollow Fiber ◽  
Inhibitory Activity ◽  
Ph Value ◽  
Enzymatic Reaction ◽  
Amino Acid Residues ◽  
Reaction Conditions ◽  
Docking Analysis ◽  
Dopamine Concentration ◽  
Immobilization Time

In this study, a polydopamine (PDA)-modified hollow fiber-immobilized xanthine oxidase (XOD) was prepared for screening potential XOD inhibitors from flavonoids. Several parameters for the preparation of PDA-modified hollow fiber-immobilized XOD, including the dopamine concentration, modification time, XOD concentration and immobilization time, were optimized. The results show that the optimal conditions for immobilized XOD activity were a dopamine concentration of 2.0 mg/mL in 10.0 mM Tris-HCl buffer (pH 8.5), a modification time of 3.0 h, an XOD concentration of 1000 μg/mL in 10.0 mM phosphate buffer (pH 7.5) and an immobilization time of 3.0 h. Subsequently, the enzymatic reaction conditions such as the pH value and temperature were investigated, and the enzyme kinetics and inhibition parameters were determined. The results indicate that the optimal pH value (7.5) and temperature (37 °C) of the PDA-modified hollow fiber-immobilized XOD were consistent with the free enzyme. Moreover, the PDA-modified hollow fiber-immobilized XOD could still maintain above 50% of its initial immobilized enzyme activity after seven consecutive cycles. The Michaelis–Menten constant (Km) and the half-maximal inhibitory concentration (IC50) of allopurinol on the immobilized XOD were determined as 0.25 mM and 23.2 μM, respectively. Furthermore, the PDA-modified hollow fiber-immobilized XOD was successfully applied to evaluate the inhibitory activity of eight flavonoids. Quercetin, apigenin, puerarin and epigallocatechin showed a good inhibition effect, and their percentages of inhibition were (79.86 ± 3.50)%, (80.98 ± 0.64)%, (61.15 ± 6.26)% and (54.92 ± 0.41)%, respectively. Finally, molecular docking analysis further verified that these four active compounds could bind to the amino acid residues in the XOD active site. In summary, the PDA-modified hollow fiber-immobilized XOD is an efficient method for the primary screening of XOD inhibitors from natural products.

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