Protective Effects of Degraded Soybean Polysaccharides on Renal Epithelial Cells Exposed to Oxidative Damage

2016 ◽  
Vol 64 (42) ◽  
pp. 7911-7920 ◽  
Author(s):  
Xin-Yuan Sun ◽  
Jian-Ming Ouyang ◽  
Poonam Bhadja ◽  
Qin Gui ◽  
Hua Peng ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Oxidative Damage ◽  
Protective Effects ◽  
Renal Epithelial Cells

Prior heat stress enhances survival of renal epithelial cells after ATP depletion

1996 ◽  
Vol 270 (6) ◽  
pp. F1057-F1065 ◽  
Author(s):  
Y. H. Wang ◽  
S. C. Borkan
Keyword(s):  
Heat Stress ◽  
Epithelial Cells ◽  
Recovery Period ◽  
Atp Depletion ◽  
Protective Effects ◽  
Atp Content ◽  
Renal Epithelial Cells ◽  
Opossum Kidney ◽  
Ok Cells

The 72-kDa heat stress protein (HSP-72) is an inducible cytoprotectant protein. Although transient renal ischemia in vivo induces HSP-72, it is not known whether prior heat stress protects renal epithelial cells from injury mediated by ATP depletion. To evaluate this hypothesis, opossum kidney (OK) cells were exposed to sodium cyanide and 2-deoxy-D-glucose in the absence of medium glucose, a maneuver that reduced cell ATP content to < 10% of the control value within 10 min and decreased cell survival. One day after 2 h of ATP depletion, OK cells previously exposed to heat stress (to induce accumulation of HSP-72) exhibited marked improvement in survival (a > 4-fold increase in total DNA), less uptake of vital dye, and less release of lactate dehydrogenase (LDH) than cells subjected to ATP depletion alone (23.0 +/- 1.6 vs. 34.1 +/- 1.2% of total LDH, respectively). Enhanced clonogenicity post-heat stress was completely prevented by cycloheximide and positively correlated with the steady-state content of HSP-72. In the recovery period after ATP depletion, cell ATP content, maximum mitochondrial ATP production rate, and total LDH activity were all significantly higher in cells with abundant HSP-72. Although the protective effects associated with heat stress are likely to be multifactoral, preserved cell metabolism and higher ATP content could enhance cellular repair processes after ATP depletion.

scholarly journals Protective Effect of Degraded Porphyra yezoensis Polysaccharides on the Oxidative Damage of Renal Epithelial Cells and on the Adhesion and Endocytosis of Nanocalcium Oxalate Crystals

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Qian-Long Peng ◽  
Chuang-Ye Li ◽  
Yao-Wang Zhao ◽  
Xin-Yuan Sun ◽  
Hong Liu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Epithelial Cells ◽  
Oxidative Damage ◽  
Protective Effect ◽  
Calcium Oxalate ◽  
Kidney Stone ◽  
Stone Formation ◽  
Porphyra Yezoensis ◽  
Renal Epithelial Cells ◽  
Kidney Stone Formation

The protective effects of Porphyra yezoensis polysaccharides (PYPs) with molecular weights of 576.2 (PYP1), 105.4 (PYP2), 22.47 (PYP3), and 3.89 kDa (PYP4) on the oxidative damage of human kidney proximal tubular epithelial (HK-2) cells and the differences in adherence and endocytosis of HK-2 cells to calcium oxalate monohydrate crystals before and after protection were investigated. Results showed that PYPs can effectively reduce the oxidative damage of oxalic acid to HK-2 cells. Under the preprotection of PYPs, cell viability increased, cell morphology improved, reactive oxygen species levels decreased, mitochondrial membrane potential increased, S phase cell arrest was inhibited, the cell apoptosis rate decreased, phosphatidylserine exposure reduced, the number of crystals adhered to the cell surface reduced, but the ability of cells to endocytose crystals enhanced. The lower the molecular weight, the better the protective effect of PYP. The results in this article indicated that PYPs can reduce the risk of kidney stone formation by protecting renal epithelial cells from oxidative damage and reducing calcium oxalate crystal adhesion, and PYP4 with the lowest molecular weight may be a potential drug for preventing kidney stone formation.

Protective Effects of Nicotinamide Riboside on H2O2-induced Oxidative Damage in Lens Epithelial Cells

2020 ◽  
pp. 1-10
Author(s):  
Biting Zhou ◽  
Guangyu Zhao ◽  
Yihua Zhu ◽  
Xiaole Chen ◽  
Nanwen Zhang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Oxidative Damage ◽  
Lens Epithelial Cells ◽  
Protective Effects ◽  
Nicotinamide Riboside

Repair activity and crystal adhesion inhibition of polysaccharides with different molecular weights from red algae Porphyra yezoensis against oxalate-induced oxidative damage in renal epithelial cells

2019 ◽  
Vol 10 (7) ◽  
pp. 3851-3867 ◽  
Author(s):  
Xin-Yuan Sun ◽  
Hui Zhang ◽  
Jie Liu ◽  
Jian-Ming Ouyang
Keyword(s):  
Epithelial Cells ◽  
Cell Surface ◽  
Oxidative Damage ◽  
Red Algae ◽  
Porphyra Yezoensis ◽  
Renal Epithelial Cells ◽  
Molecular Weights ◽  
Repair Activity ◽  
Crystal Adhesion ◽  
Adhesion Inhibition

Porphyra yezoensis polysaccharide repaired oxalate-injured renal epithelial cells and decreased COM crystal adhesion on the cell surface.

Melatonin reduces high levels of lipid peroxidation induced by potassium iodate in porcine thyroid

2019 ◽  
pp. 1-7 ◽  
Author(s):  
Paulina Iwan ◽  
Jan Stepniak ◽  
Malgorzata Karbownik-Lewinska
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Damage ◽  
Membrane Lipids ◽  
Chemical Properties ◽  
Iodine Concentration ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Protective Effects ◽  
Potassium Iodate ◽  
Hormone Synthesis

Abstract. Iodine is essential for thyroid hormone synthesis. Under normal iodine supply, calculated physiological iodine concentration in the thyroid is approx. 9 mM. Either potassium iodide (KI) or potassium iodate (KIO3) are used in iodine prophylaxis. KI is confirmed as absolutely safe. KIO3 possesses chemical properties suggesting its potential toxicity. Melatonin (N-acetyl-5-methoxytryptamine) is an effective antioxidant and free radical scavenger. Study aims: to evaluate potential protective effects of melatonin against oxidative damage to membrane lipids (lipid peroxidation, LPO) induced by KI or KIO3 in porcine thyroid. Homogenates of twenty four (24) thyroids were incubated in presence of either KI or KIO3 without/with melatonin (5 mM). As melatonin was not effective against KI-induced LPO, in the next step only KIO3 was used. Homogenates were incubated in presence of KIO3 (200; 100; 50; 25; 20; 15; 10; 7.5; 5.0; 2.5; 1.25 mM) without/with melatonin or 17ß-estradiol. Five experiments were performed with different concentrations of melatonin (5.0; 2.5; 1.25; 1.0; 0.625 mM) and one with 17ß-estradiol (1.0 mM). Malondialdehyde + 4-hydroxyalkenals (MDA + 4-HDA) concentration (LPO index) was measured spectrophotometrically. KIO3 increased LPO with the strongest damaging effect (MDA + 4-HDA level: ≈1.28 nmol/mg protein, p < 0.05) revealed at concentrations of around 15 mM, thus corresponding to physiological iodine concentrations in the thyroid. Melatonin reduced LPO (MDA + 4-HDA levels: from ≈0.97 to ≈0,76 and from ≈0,64 to ≈0,49 nmol/mg protein, p < 0.05) induced by KIO3 at concentrations of 10 mM or 7.5 mM. Conclusion: Melatonin can reduce very strong oxidative damage to membrane lipids caused by KIO3 used in doses resulting in physiological iodine concentrations in the thyroid.

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