A Vernonia Diacylglycerol Acyltransferase Can Increase Renewable Oil Production

2016 ◽  
Vol 64 (38) ◽  
pp. 7188-7194 ◽  
Author(s):  
Tomoko Hatanaka ◽  
William Serson ◽  
Runzhi Li ◽  
Paul Armstrong ◽  
Keshun Yu ◽  
...  
Keyword(s):  
Oil Production ◽  
Diacylglycerol Acyltransferase

scholarly journals Overexpression of Type 1 and 2 Diacylglycerol Acyltransferase Genes (JcDGAT1 and JcDGAT2) Enhances Oil Production in the Woody Perennial Biofuel Plant Jatropha curcas

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 699
Author(s):  
Tian-Tian Zhang ◽  
Huiying He ◽  
Chuan-Jia Xu ◽  
Qiantang Fu ◽  
Yan-Bin Tao ◽  
...  
Keyword(s):  
Jatropha Curcas ◽  
Soluble Sugars ◽  
Oil Production ◽  
Dry Weight ◽  
Diacylglycerol Acyltransferase ◽  
Seed Kernel ◽  
Woody Perennial ◽  
Late Stages

Diacylglycerol acyltransferase (DGAT) is the only enzyme that catalyzes the acyl-CoA-dependent acylation of sn-1, 2-diacylglycerol (DAG) to form triacylglycerol (TAG). The two main types of DGAT enzymes in the woody perennial biofuel plant Jatropha curcas, JcDGAT1 and JcDGAT2, were previously characterized only in heterologous systems. In this study, we investigated the functions of JcDGAT1 and JcDGAT2 in J. curcas.JcDGAT1 and JcDGAT2 were found to be predominantly expressed during the late stages of J. curcas seed development, in which large amounts of oil accumulated. As expected, overexpression of JcDGAT1 or JcDGAT2 under the control of the CaMV35S promoter gave rise to an increase in seed kernel oil production, reaching a content of 53.7% and 55.7% of the seed kernel dry weight, respectively, which were respectively 25% and 29.6% higher than that of control plants. The increase in seed oil content was accompanied by decreases in the contents of protein and soluble sugars in the seeds. Simultaneously, there was a two- to four-fold higher leaf TAG content in transgenic plants than in control plants. Moreover, by analysis of the fatty acid (FA) profiles, we found that JcDGAT1 and JcDGAT2 had the same substrate specificity with preferences for C18:2 in seed TAGs, and C16:0, C18:0, and C18:1 in leaf TAGs. Therefore, our study confirms the important role of JcDGAT1 and JcDGAT2 in regulating oil production in J. curcas.

Functional Characterization of Three Novel Genes Encoding Diacylglycerol Acyltransferase (DGAT) from Oil-Rich Tubers of Cyperus esculentus

2019 ◽  
Vol 61 (1) ◽  
pp. 118-129 ◽  
Author(s):  
Dantong Liu ◽  
Hongying Ji ◽  
Zhenle Yang
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Functional Characterization ◽  
Oil Production ◽  
Diacylglycerol Acyltransferase ◽  
Type I ◽  
Cyperus Esculentus ◽  
Wild Type ◽  
Oil Synthesis ◽  
Genes Encoding

Abstract Cyperus esculentus is probably the only plant that is known to accumulate large amounts of oil in its tubers. However, the underlying metabolic mechanism and regulatory factors involved in oil synthesis of tubers are still largely unclear. In this study, one gene encoding type I diacylglycerol acyltransferase (DGAT) (CeDGAT1) and two genes encoding type II DGAT (CeDGAT2a and CeDGAT2b) from C. esculentus were identified and functionally analyzed. All three DGAT genes were found to be expressed in tuber, root and leaf tissues. CeDGAT1 is highly expressed in roots and leaves, whereas CeDGAT2b is dominantly expressed in tubers. Furthermore, the temporal expression pattern of CeDGAT2b is well coordinated with the oil accumulation in developing tubers. When each CeDGAT was heterologously expressed in triacylglycerol (TAG)-deficient mutant of Saccharomyces cerevisiae, Arabidopsis thaliana wild type or its TAG1 mutant with AtDGAT1 disruption, only CeDGAT2b showed the ability to restore TAG biosynthesis with lipid body formation in yeast mutant, enhance seed oil production of Arabidopsis wild type and rescue multiple seed phenotypes of TAG1 mutant. In addition, CeDGAT2b was shown to have a substrate preference for unsaturated fatty acids toward TAG synthesis. Taken together, our results indicated that CeDGAT2b from C. esculentus is an actively functional protein and is most likely the major contributor to tuber oil biosynthesis containing common fatty acids, in contrast to oil-rich seeds and fruits where DGAT1 plays a more central role than DGAT2 in oil production accumulating normal fatty acids, whereas DGAT2 is a primary regulator for oil synthesis rich in unusual fatty acids.

Russian Oil: Production and Exports

2003 ◽  
pp. 136-146
Author(s):  
K. Liuhto
Keyword(s):  
Baltic Sea ◽  
Statistical Data ◽  
Oil Production ◽  
The Baltic Sea ◽  
North West ◽  
The North ◽  
Oil Transportation ◽  
Sea Ports ◽  
The Baltic

Statistical data on reserves, production and exports of Russian oil are provided in the article. The author pays special attention to the expansion of opportunities of sea oil transportation by construction of new oil terminals in the North-West of the country and first of all the largest terminal in Murmansk. In his opinion, one of the main problems in this sphere is prevention of ecological accidents in the process of oil transportation through the Baltic sea ports.

Reusability Study of Sulfated Zirconia Functionalized SBA-15 Catalyst for Biolubricant Oil Production from Oleic Acid

2019 ◽  
Vol 1 (1) ◽  
pp. 46
Author(s):  
F R Rangganita ◽  
L Hermida ◽  
A Angraeni ◽  
D Khoirunnisa
Keyword(s):  
Oleic Acid ◽  
Sulfated Zirconia ◽  
Room Temperature ◽  
Oil Production ◽  
Catalyst Reusability

Sulfated zirconia functionalized SBA-15 catalsyt (SZr-SBA-15) was prepared byreacting SBA-15 with Zirkoniumoxychloride and urea at 90oC to form ZrO2-SBA-15. Then, ZrO2-SBA-15 was reacted with H2SO4 at room temperature to produceSZr-SBA-15 catalsyt.. The catalyst was characterized in terms of adsorptiondesorption nitrogen analysis, SEM-EDX and FTIR. Based on SEM-EDX andadsorption-desorption nitrogen analysis results, it was found that Zr had beenincorporated in SBA-15. By using the SZr-SBA-15 catalyst, esterification reactionof oleic acid with TMP to produce biolubricant oil of Trimethylolpropanetrioleatachieved 85% oleic acid conversion and selectivity of 63,7%. Reusability study ofSZr-SBA-15 catalyst was carried out for 3 rounds of reaction. It was found that thecatalyst could be used up to 3 rounds without significant decrease in activityKeywords: biolubricant oil, catalyst reusability, sba-15, sulfated zirconia.

Optimization of well product pumpless transportation under offshore oil production conditions

2017 ◽  
pp. 140-142
Author(s):  
A.G. Akhmadeev ◽  
◽  
Pham Thanh Vinh ◽  
Bui Trong Han ◽  
Le Huu Toan ◽  
...  
Keyword(s):  
Oil Production ◽  
Offshore Oil Production ◽  
Offshore Oil ◽  
Production Conditions

Creation of the ‘Internet of things’ in crude oil production

2017 ◽  
Vol 10 ◽  
pp. 120-124
Author(s):  
R.S. Khisamov ◽  
◽  
R.A. Gabdrahmanov ◽  
A.P. Bespalov ◽  
V.V. Zubarev ◽  
...  
Keyword(s):  
Internet Of Things ◽  
Crude Oil ◽  
Oil Production ◽  
The Internet ◽  
Crude Oil Production ◽  
The Internet Of Things
Sign in / Sign up

Export Citation Format

Share Document