Anti-hyaluronidase Activity in Vitro and Amelioration of Mouse Experimental Dermatitis by Tomato Saponin, Esculeoside A

2016 ◽  
Vol 64 (2) ◽  
pp. 403-408 ◽  
Author(s):  
Jian-Rong Zhou ◽  
Yurina Kanda ◽  
Anna Tanaka ◽  
Hideyuki Manabe ◽  
Toshihiro Nohara ◽  
...  
Keyword(s):  
Hyaluronidase Activity ◽  
Esculeoside A

scholarly journals Competitive Inhibition of Mammalian Hyaluronidase by Tomato Saponin, Esculeoside A

2018 ◽  
Vol 13 (11) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Jian-Rong Zhou ◽  
Souta Kimura ◽  
Toshihiro Nohara ◽  
Kazumi Yokomizo
Keyword(s):  
Hyaluronic Acid ◽  
Kinetic Analysis ◽  
Competitive Inhibition ◽  
Tomato Fruits ◽  
Hyaluronidase Activity ◽  
Significant Difference ◽  
Esculeoside A ◽  
Menten Constant

Esculeoside A, a glycoside of spirosolane-type, is identified as a major component in ripe tomato fruits. Our previous study showed that esculeoside A inhibited hyaluronidase activity in vitro and ameliorated experimental dermatitis in vivo. The aim of this present study is to investigate the inhibition mode on mammalian hyaluronidase by esculeoside A. Measured by a modified Morgan-Elson method, the present kinetic analysis of the hydrolysis reaction using hyaluronic acid revealed that no significant difference was observed in velocity maximum Vmax, and Michaelis-Menten constant Km was shown as 0.74 mM in the absence of esculeoside A, was increased as 1.32 and 1.98 mM with 3 and 30 μM of esculeoside A, respectively. Thus suggested that the inhibition mode on hyaluronidase by esculeoside A was competitive. This competitive inhibition on hyaluronidase activity may become valuable in the amelioration of mice experimental dermatitis by esculeoside A.

scholarly journals STUDIES ON SPREADING FACTORS

1947 ◽  
Vol 86 (1) ◽  
pp. 19-28 ◽  
Author(s):  
Oscar Hechter ◽  
Ella L. Scully
Keyword(s):  
Environmental Conditions ◽  
Defense Mechanism ◽  
Normal Serum ◽  
Inhibitory Factor ◽  
Inhibitory Influence ◽  
Serum Factor ◽  
Hyaluronidase Activity

The reaction between normal serum and hyaluronidase has been studied in vitro and under in vivo conditions in skin. Using in vitro conditions of incubation, serum exhibits antihyaluronidase activity as measured by assay of hyaluronidase spreading activity in skin. This confirms the work of others, who have previously described the serum inhibitory factor using other tests of hyaluronidase activity. When, however, hyaluronidase and setum are allowed to incubate in skin under in vivo conditions, no inhibitory influence of serum upon hyaluronidase spreading activity is evident. This latter finding has been taken to indicate that the environmental conditions in skin are unfavorable for the inhibitory reaction of serum upon hyaluronidase. The disparity between the in vivo and in vitro effectiveness of serum, and the significance of the serum factor as a defense mechanism against invasive processes, have been briefly discussed.

Inhibition of hyaluronidase activity of human and rat spermatozoa in vitro and antispermatogenic activity in rats in vivo by Terminalia chebula, a flavonoid rich plant

2010 ◽  
Vol 29 (2) ◽  
pp. 214-224 ◽  
Author(s):  
Archana Srivastav ◽  
Abhishek Chandra ◽  
Madhulika Singh ◽  
Farrukh Jamal ◽  
Preeti Rastogi ◽  
...  
Keyword(s):  
Terminalia Chebula ◽  
Hyaluronidase Activity

Investigation of Aminomethyl Indole Derivatives as Hyaluronidase Inhibitors

2010 ◽  
Vol 65 (7-8) ◽  
pp. 445-450 ◽  
Author(s):  
Süreyya Ölgen ◽  
Andre Kaessler ◽  
Zühal Zühal Kılıç-Kurt ◽  
Joachim Jose
Keyword(s):  
Bacterial Infections ◽  
The Other ◽  
In Vitro Assays ◽  
Indole Derivatives ◽  
Malignant Tumours ◽  
Lipophilic Compounds ◽  
Hyaluronidase Activity ◽  
Therapeutic Value

Hyaluronidase inhibitors are of potential therapeutic value for the treatment of a variety of diseases, such as cancer, arthrosis, or bacterial infections. Potent and selective hyaluronidase inhibitors are not known so far, and current approaches to the development of hyaluronidase inhibitors are limited. Elevated levels of hyaluronan (HA) are connected with most malignant tumours. Therefore, the search for drugs modulating the hyaluronidase activity became very important. In the present study, a new series of aminomethyl indole derivatives (AMIDs) were tested for inhibition of bovine testes hyaluronidase (BTH). In vitro assays were performed using stains-all at pH 7 and Morgan-Elson reaction at pH 3.5. Among the AMIDs, 3-[(4-methylpiperazin-1-yl)methyl]-5-phenyl-1H-indole (9) was found to be active with 23% inhibition at 50 μM and pH 7. All the other inhibitors showed less activity at pH 3.5 and pH 7. These activity results demonstrated that compounds with phenyl substitution at position 5 have higher activity. The results confirmed that more lipophilic compounds have better inhibition against the hyaluronidase enzyme.

scholarly journals Anti-Ageing Potential of S. euboea Heldr. Phenolics

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3151
Author(s):  
Ekaterina-Michaela Tomou ◽  
Christina D. Papaemmanouil ◽  
Dimitrios A. Diamantis ◽  
Androniki D. Kostagianni ◽  
Paschalina Chatzopoulou ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Early Stage ◽  
Chemical Constituents ◽  
Docking Simulation ◽  
In Vitro Study ◽  
2D Nmr ◽  
In Silico Docking ◽  
Hyaluronidase Activity ◽  
Bioactive Agents

In recent years, the use of Sideritis species as bioactive agents is increasing exponentially. The present study aimed to investigate the chemical constituents, as well as the anti-ageing potential of the cultivated Sideritis euboea Heldr. The chemical fingerprinting of the ethyl acetate residue of this plant was studied using 1D and 2D-NMR spectra. Isomeric compounds belonging to acylated flavone derivatives and phenylethanoid glycosides were detected in the early stage of the experimental process through 2D-NMR techniques. Overall, thirty-three known compounds were isolated and identified. Some of them are reported for the first time not only in S. euboea, but also in genus Sideritis L. The anti-ageing effect of the ethyl acetate residue and the isolated specialized products was assessed as anti-hyaluronidase activity. In silico docking simulation revealed the interactions of the isolated compounds with hyaluronidase. Furthermore, the in vitro study on the inhibition of hyaluronidase unveiled the potent inhibitory properties of ethyl acetate residue and apigenin 7-O-β-d-glucopyranoside. Though, the isomers of apigenin 7-O-p-coumaroyl-glucosides and also the 4′-methyl-hypolaetin 7-O-[6′′′-O-acetyl-β-d-allopyranosyl]-(1→2)-β-d-glucopyranoside exerted moderate hyaluronidase inhibition. This research represents the first study to report on the anti-hyaluronidase activity of Sideritis species, confirming its anti-inflammatory, cytotoxic and anti-ageing effects and its importance as an agent for cosmetic formulations as also anticancer potential.

scholarly journals In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.)

2014 ◽  
Vol 4 (12) ◽  
pp. 959-963 ◽  
Author(s):  
Wanigasekera Daya Ratnasooriya ◽  
Walimuni Prabhashini Kaushalya Mend Abeysekera ◽  
Chatura Tissa Dayendra Ratnasooriya
Keyword(s):  
Camellia Sinensis ◽  
Black Tea ◽  
Sri Lankan ◽  
Hyaluronidase Activity

scholarly journals Enhancement of the Antioxidant, Anti-Tyrosinase, and Anti-Hyaluronidase Activity of Morus alba L. Leaf Extract by Pulsed Electric Field Extraction

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2212
Author(s):  
Wantida Chaiyana ◽  
Jakkapan Sirithunyalug ◽  
Suvimol Somwongin ◽  
Chanun Punyoyai ◽  
Natnaree Laothaweerungsawat ◽  
...  
Keyword(s):  
Electric Field ◽  
Leaf Extract ◽  
Phenolic Content ◽  
Pulsed Electric Field ◽  
Morus Alba ◽  
Total Phenolic ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Hyaluronidase Activity

In this study we aimed to compare the chemical composition and biological activity between Morus alba L. leaf extract obtained with 95% v/v ethanol using a pulsed electric field (PEF) and the conventional maceration method. Extracts of M. alba leaves collected from Chiang Mai (CM), Sakon Nakon (SK), and Buriram (BR), Thailand, were investigated for 1-deoxynojirimycin content by high-performance liquid chromatography and for total phenolic content by the Folin–Ciocalteu method. Antioxidant activity was investigated by 2,2′-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azinobis-3-ethylbenzothiazoline-6-sulphonate (ABTS), and ferric reducing antioxidant power (FRAP) assay. Anti-tyrosinase and anti-hyaluronidase activity was investigated by in vitro spectrophotometry. The results show that this is the first study to indicate PEF as a novel method for enhancing the phenolic content and antioxidant, anti-tyrosinase, and anti-hyaluronidase activity of M. alba leaf extract (P < 0.05). PEF extract of M. alba leaves collected from BR had comparable ABTS•+ scavenging activity to l-ascorbic acid and comparable anti-tyrosinase activity to kojic acid (P > 0.05). On the other hand, PEF extract of M. alba leaves collected from SK exhibited significantly high anti-hyaluronidase activity, comparable to that of oleanolic acid (P > 0.05). Therefore, PEF is suggested for further M. alba leaf extraction in the production of natural whitening and anti-aging cosmetic ingredients.

Hyaluronidase activity of macaque sperm assessed by an in vitro cumulus penetration assay

1997 ◽  
Vol 46 (3) ◽  
pp. 392-400 ◽  
Author(s):  
Stuart A. Meyers ◽  
Ashley I. Yudin ◽  
Gary N. Cherr ◽  
Catherine A. VandeVoort ◽  
Diana G. Myles ◽  
...  
Keyword(s):  
Hyaluronidase Activity

Inhibition of boar sperm hyaluronidase activity by tannic acid reduces polyspermy during in vitro fertilization of porcine oocytes

Zygote ◽  
2006 ◽  
Vol 14 (4) ◽  
pp. 275-285 ◽  
Author(s):  
Hideki Tatemoto ◽  
Isao Tokeshi ◽  
Satoshi Nakamura ◽  
Norio Muto ◽  
Tadashi Nakada
Keyword(s):  
Tannic Acid ◽  
Penetration Rate ◽  
Vitro Fertilization ◽  
Hyaluronidase Activity ◽  
High Penetration ◽  
Boar Sperm ◽  
Dependent Inhibition ◽  
Dose Dependent Inhibition ◽  
Dose Dependent

SummaryThe present study was conducted to examine the effects of three polyphenols (tannic acid, apigenin and quercetin) on hyaluronidase activity and in vitro fertilization (IVF) parameters. Among them, tannic acid showed by far the strongest potency for blocking hyaluronidase activity extracted from preincubated boar sperm, causing a dose-dependent inhibition over the range of 2–10 μg/ml. When cumulus-intact and cumulus-free oocytes were inseminated in IVF medium containing tannic acid, the penetration and the polyspermy rates were significantly decreased in the presence of 10 μg/ml tannic acid compared with those in the absence of tannic acid, and the addition of 5 μg/ml tannic acid significantly reduced the polyspermy rate (p < 0.05) compared with that of the control while maintaining the high penetration rate. However, apigenin and quercetin had no effect on the rate of polyspermy. Interestingly, the incidence of polyspermy was significantly reduced in oocytes inseminated with sperm pretreated with 5 μg/ml tannic acid (p < 0.05), although the pretreatment of oocytes had no effect against the polyspermy after insemination with untreated sperm. Treatment with tannic acid caused neither a protective proteolytic modification of the zona pellucida matrix before fertilization, nor a reduction of the proteolytic activity of acrosomal contents or the number of zona-bound spermatozoa. These data suggest that an appropriate concentration of tannic acid prevents polyspermy through the inhibition of sperm hyaluronidase activity during IVF of porcine oocytes.

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