Flavourzyme, an Enzyme Preparation with Industrial Relevance: Automated Nine-Step Purification and Partial Characterization of Eight Enzymes

2015 ◽  
Vol 63 (23) ◽  
pp. 5682-5693 ◽  
Author(s):  
Michael Merz ◽  
Thomas Eisele ◽  
Pieter Berends ◽  
Daniel Appel ◽  
Swen Rabe ◽  
...  
Keyword(s):  
Enzyme Preparation ◽  
Partial Characterization ◽  
Industrial Relevance

The assay and partial characterization of 3β-hydroxysteroid sulfotransferase of the hamster epididymis

1985 ◽  
Vol 63 (1) ◽  
pp. 71-76 ◽  
Author(s):  
M. Bouthillier ◽  
G. Bleau ◽  
A. Chapdelaine ◽  
K. D. Roberts
Keyword(s):  
Organic Phase ◽  
Enzyme Preparation ◽  
Enzymatic Reaction ◽  
Partial Characterization ◽  
Optimal Conditions ◽  
Steroid Nucleus ◽  
Optimum Ph ◽  
High Concentrations

Using a partially purified enzyme preparation obtained from hamster epididymis, a simple assay has been developed to measure the sulfurylation of dehydroisoandrosterone (DHA) and desmosterol in the presence of 3′-phosphoadenosine 5′-phospho[35S]sulfate ([35S]PAPS). After stopping the enzymatic reaction with methanol and KCl, the 35S-labelled steroid sulfates are readily extracted into an organic phase. Optimal conditions for the sulfurylation of the two steroids were compared; optimum pH is 8.7 for DHA and 9.8 for desmosterol. Sulfoconjugation of desmosterol increases with magnesium concentrations up to 6 mM, while 40 mM concentrations of the divalent ion are required for the optimal sulfurylation of DHA. Maximum sulfurylation of these steroids requires the presence of 15 mM cysteine. Michaelis–Menten kinetics are observed with DHA which has an apparent Km of 32 μM, while desmosterol inhibits sulfotransferase activity at high concentrations. Saturation of the enzyme with PAPS results in an allosteric behaviour. Only the 3β-hydroxyl function of the steroid nucleus appears to be an appropriate sulfate acceptor for the epididymal hydroxysteroid sulfotransferase.

Multiple forms of glycosidases in an enzyme preparation from Aspergillus niger: Partial characterization of a β-apiosidase

1997 ◽  
Vol 21 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Ziya Günata ◽  
Isabelle Dugelay ◽  
M.J. Vallier ◽  
J.C. Sapis ◽  
C. Bayonove
Keyword(s):  
Aspergillus Niger ◽  
Enzyme Preparation ◽  
Partial Characterization ◽  
Multiple Forms

Partial characterization of the N-linked oligosaccharides occurring on rat hepatocyte glycoproteins

2010 ◽  
Vol 108 (10) ◽  
pp. 323-329 ◽  
Author(s):  
Marti F. A. Bierhuizen ◽  
Moniek de Wit ◽  
Carin A. R. L. Govers ◽  
Willem van Dijk
Keyword(s):  
Partial Characterization ◽  
Rat Hepatocyte

scholarly journals Localization and Partial Characterization of an Aldehydic Component in Tropocollagen

1966 ◽  
Vol 241 (7) ◽  
pp. 1530-1536
Author(s):  
Marcos Rojkind ◽  
Olga O. Blumenfeld ◽  
Paul M. Gallop
Keyword(s):  
Partial Characterization

scholarly journals Detection and partial characterization of a protein in mouse fibroblasts that binds and inhibits trypsin.

1980 ◽  
Vol 255 (11) ◽  
pp. 5468-5474 ◽  
Author(s):  
R.J. Kirschner ◽  
C.S. Federiuk ◽  
J.P. Ford ◽  
J.A. Shafer
Keyword(s):  
Partial Characterization ◽  
Mouse Fibroblasts
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