Whole-Cell Biocatalyst for Rubusoside Production in Saccharomyces cerevisiae

2021 ◽  
Author(s):  
Yaping Mao ◽  
Zhuo Chen ◽  
Yuhong Ren ◽  
Yuwei Sun ◽  
Yong Wang
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Whole Cell ◽  
Whole Cell Biocatalyst

scholarly journals Enzymatic glutathione production using metabolically engineered Saccharomyces cerevisiae as a whole-cell biocatalyst

2011 ◽  
Vol 91 (4) ◽  
pp. 1001-1006 ◽  
Author(s):  
Hideyo Yoshida ◽  
Kiyotaka Y. Hara ◽  
Kentaro Kiriyama ◽  
Hideki Nakayama ◽  
Fumiyoshi Okazaki ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
Engineered Saccharomyces Cerevisiae

Baker’s yeast catalyzed one-pot synthesis of bioactive 2-[benzylidene(or pyrazol-4-ylmethylene)hydrazono]-1,3-thiazolidin-4-one-5-yl-acetic acids

2018 ◽  
Vol 24 (2) ◽  
pp. 103-107 ◽  
Author(s):  
Anusaya S. Chavan ◽  
Arun S. Kharat ◽  
Manisha R. Bhosle ◽  
Ramrao A. Mane
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Maleic Anhydride ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
One Pot ◽  
Whole Cell ◽  
One Pot Synthesis ◽  
Whole Cell Biocatalyst ◽  
Derivatives Of ◽  
Acetic Acids

Abstract An efficient and simple one-pot protocol has been developed for synthesis of substituted derivatives of 2-hydrazono-4-thiazolidinone-5-acetic acids 4a–j and 6a–g by cyclocondensation of aryl/pyrazolyl aldehyde, thiosemicarbazide and maleic anhydride in acetonitrile in the presence of readily available whole cell biocatalyst, baker’s yeast (Saccharomyces cerevisiae). The reaction is enhanced by ultrasonication.

scholarly journals IMPROVEMENT OF BIOETHANOL PRODUCTION BY USING Saccharomyces cerevisiae [Meyen ex E.C. Hansen] IMMOBILIZED ON PRETREATED SUGARCANE BAGASSE

REAKTOR ◽  
2018 ◽  
Vol 18 (03) ◽  
pp. 127 ◽  
Author(s):  
Sita Heris Anita ◽  
Wibowo Mangunwardoyo ◽  
Yopi Yopi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Physical Properties ◽  
Sugarcane Bagasse ◽  
Immobilized Cells ◽  
Ethanol Yield ◽  
Steam Treatment ◽  
Bioethanol Production ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
Pretreated Sugarcane Bagasse

Pretreated of sugarcane bagasse was used as a carrier for immobilization of Saccharomyces cerevisiae. Pretreatments were carried out by steaming, pressurized steam, and combination both of procedure.  The objectives of this research was to investigate the effect of pretreatment on sugarcane bagasse to cells adsorption and bioethanol production.  Immobilization process was conducted in a ratio of 2.5 g carrier/50 mL cell suspension.  Whole cell biocatalyst as much as 1% (w/v) was used as inoculum for bioethanol fermentation.  The best pretreated sugarcane bagasse for carrier of immobilized cells was obtained using steam treatment for 30 minutes.  Those treatment improved the physical properties of carrier and increased the cell retention up to 10.05 mg/g.  The use of whole cell biocatalyst after steaming pretreatment also enhanced ethanol yield 1.5 times higher than control. Keywords: bioethanol; immobilization; pretreatment; steam treatment; sugarcane bagasse

Construction of whole-cell biocatalyst for xylan degradation through cell-surface xylanase display in Saccharomyces cerevisiae

2002 ◽  
Vol 17 (3-5) ◽  
pp. 189-195 ◽  
Author(s):  
Yasuya Fujita ◽  
Satoshi Katahira ◽  
Mitsuyoshi Ueda ◽  
Atsuo Tanaka ◽  
Hirofumi Okada ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Surface ◽  
Whole Cell ◽  
Xylan Degradation ◽  
Whole Cell Biocatalyst

scholarly journals Tuning a bi-enzymatic cascade reaction in Escherichia coli to facilitate NADPH regeneration for ε-caprolactone production

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jinghui Xiong ◽  
Hefeng Chen ◽  
Ran Liu ◽  
Hao Yu ◽  
Min Zhuo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Sites ◽  
Regeneration System ◽  
Nadph Regeneration ◽  
Whole Cell ◽  
Cyclohexanone Monooxygenase ◽  
Ribosome Binding ◽  
Ribosome Binding Sites ◽  
Whole Cell Biocatalyst ◽  
Substrate Tolerance

Abstractε-Caprolactone is a monomer of poly(ε-caprolactone) which has been widely used in tissue engineering due to its biodegradability and biocompatibility. To meet the massive demand for this monomer, an efficient whole-cell biocatalytic approach was constructed to boost the ε-caprolactone production using cyclohexanol as substrate. Combining an alcohol dehydrogenase (ADH) with a cyclohexanone monooxygenase (CHMO) in Escherichia coli, a self-sufficient NADPH-cofactor regeneration system was obtained. Furthermore, some improved variants with the better substrate tolerance and higher catalytic ability to ε-caprolactone production were designed by regulating the ribosome binding sites. The best mutant strain exhibited an ε-caprolactone yield of 0.80 mol/mol using 60 mM cyclohexanol as substrate, while the starting strain only got a conversion of 0.38 mol/mol when 20 mM cyclohexanol was supplemented. The engineered whole-cell biocatalyst was used in four sequential batches to achieve a production of 126 mM ε-caprolactone with a high molar yield of 0.78 mol/mol.

scholarly journals Development and Characterization of Two Types of Surface Displayed Levansucrases for Levan Biosynthesis

Catalysts ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 757
Author(s):  
Huiyi Shang ◽  
Danni Yang ◽  
Dairong Qiao ◽  
Hui Xu ◽  
Yi Cao
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Weight ◽  
Large Scale ◽  
Surface Display ◽  
Yeast Surface Display ◽  
Fusion Partner ◽  
Whole Cell ◽  
Food Industries ◽  
Yeast Surface ◽  
The Stability

Levan has wide applications in chemical, cosmetic, pharmaceutical and food industries. The free levansucrase is usually used in the biosynthesis of levan, but the poor reusability and low stability of free levansucrase have limited its large-scale use. To address this problem, the surface-displayed levansucrase in Saccharomyces cerevisiae were generated and evaluated in this study. The levansucrase from Zymomonas mobilis was displayed on the cell surface of Saccharomyces cerevisiae EBY100 using a various yeast surface display platform. The N-terminal fusion partner is based on a-agglutinin, and the C-terminal one is Flo1p. The yield of levan produced by these two whole-cell biocatalysts reaches 26 g/L and 34 g/L in 24 h, respectively. Meanwhile, the stability of the surface-displayed levansucrases is significantly enhanced. After six reuses, these two biocatalysts retained over 50% and 60% of their initial activities, respectively. Furthermore, the molecular weight and polydispersity test of the products suggested that the whole-cell biocatalyst of levansucrase displayed by Flo1p has more potentials in the production of levan with low molecular weight which is critical in certain applications. In conclusion, our method not only enable the possibility to reuse the enzyme, but also improves the stability of the enzyme.

Antityrosinase Mechanism and Antimelanogenic Effect of Arbutin Esters Synthesis Catalyzed by Whole-Cell Biocatalyst

2021 ◽  
Vol 69 (14) ◽  
pp. 4243-4252
Author(s):  
Haixia Xu ◽  
Xiaofeng Li ◽  
Xuan Xin ◽  
Lan Mo ◽  
Yucong Zou ◽  
...  
Keyword(s):  
Whole Cell ◽  
Whole Cell Biocatalyst

Engineered whole-cell biocatalyst-based detoxification and detection of neurotoxic organophosphate compounds

2014 ◽  
Vol 32 (3) ◽  
pp. 652-662 ◽  
Author(s):  
Chang Sup Kim ◽  
Jeong Hyun Seo ◽  
Dong Gyun Kang ◽  
Hyung Joon Cha
Keyword(s):  
Whole Cell ◽  
Organophosphate Compounds ◽  
Whole Cell Biocatalyst
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