Enhanced Lignanamide Absorption and Antioxidative Effect of Extruded Hempseed (Cannabis sativa L.) Hull in Caco-2 Intestinal Cell Culture

2021 ◽  
Vol 69 (38) ◽  
pp. 11259-11271
Author(s):  
William Leonard ◽  
Yun Xiong ◽  
Pangzhen Zhang ◽  
Danyang Ying ◽  
Zhongxiang Fang
Keyword(s):  
Cell Culture ◽  
Cannabis Sativa ◽  
Intestinal Cell ◽  
Antioxidative Effect

scholarly journals Adenovirus Infection of Human Enteroids Reveals Interferon Sensitivity and Preferential Infection of Goblet Cells

2018 ◽  
Vol 92 (9) ◽  
Author(s):  
Mayumi K. Holly ◽  
Jason G. Smith
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Enteric Viruses ◽  
Goblet Cells ◽  
Clinical Isolates ◽  
Intestinal Cell ◽  
Intestinal Epithelial ◽  
Cell Culture System ◽  
Human Adenoviruses ◽  
Enteric Adenoviruses

ABSTRACTHuman adenoviruses (HAdV) are significant human pathogens. Although only a subset of HAdV serotypes commonly cause gastroenteritis in humans, most HAdV species replicate in the gastrointestinal tract. Knowledge of the complex interaction between HAdVs and the human intestinal epithelium has been limited by the lack of a suitable cell culture system containing relevant cell types. Recently, this need has been met by the stable and prolonged cultivation of primary intestinal epithelial cells as enteroids. Human enteroids have been used to reveal novel and interesting aspects of rotavirus, norovirus, and enterovirus replication, prompting us to explore their suitability for HAdV culture. We found that both prototype strains and clinical isolates of enteric and nonenteric HAdVs productively replicate in human enteroids. HAdV-5p, a respiratory pathogen, and HAdV-41p, an enteric pathogen, are both sensitive to type I and III interferons in human enteroid monolayers but not A549 cells. Interestingly, HAdV-5p, but not HAdV-41p, preferentially infected goblet cells. And, HAdV-5p but not HAdV-41p was potently neutralized by the enteric human alpha-defensin HD5. These studies highlight new facets of HAdV biology that are uniquely revealed by primary intestinal epithelial cell culture.IMPORTANCEEnteric adenoviruses are a significant cause of childhood gastroenteritis worldwide, yet our understanding of their unique biology is limited. Here we report robust replication of both prototype and clinical isolates of enteric and respiratory human adenoviruses in enteroids, a primary intestinal cell culture system. Recent studies have shown that other fastidious enteric viruses replicate in human enteroids. Therefore, human enteroids may provide a unified platform for culturing enteric viruses, potentially enabling isolation of a greater diversity of viruses from patients. Moreover, both the ability of interferon to restrict respiratory and enteric adenoviruses and a surprising preference of a respiratory serotype for goblet cells demonstrate the power of this culture system to uncover aspects of adenovirus biology that were previously unattainable with standard cell lines.

Investigation into the metabolism of 1,8-cineole in an intestinal cell culture model and acquisition of its immune-modulatory effect via gene expression analysis

2012 ◽  
Vol 27 (6) ◽  
pp. 405-413 ◽  
Author(s):  
Jakob Müller ◽  
Natalie Gruner ◽  
Isabella Almstätter ◽  
Frauke Kirsch ◽  
Andrea Buettner ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Culture ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Intestinal Cell ◽  
Cell Culture Model ◽  
Culture Model

scholarly journals Cannabidiol Isolated From Cannabis sativa L. Protects Intestinal Barrier From In Vitro Inflammation and Oxidative Stress

2021 ◽  
Vol 12 ◽  
Author(s):  
Veronica Cocetta ◽  
Paolo Governa ◽  
Vittoria Borgonetti ◽  
Mattia Tinazzi ◽  
Gregorio Peron ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cannabis Sativa ◽  
Intestinal Barrier ◽  
Intestinal Cell ◽  
Ros Production ◽  
Herbal Products ◽  
Bowel Diseases ◽  
Current Therapies ◽  
And Oxidative Stress

The relevance and incidence of intestinal bowel diseases (IBD) have been increasing over the last 50 years and the current therapies are characterized by severe side effects, making essential the development of new strategies that combine efficacy and safety in the management of human IBD. Herbal products are highly considered in research aimed at discovering new approaches for IBD therapy and, among others, Cannabis sativa L. has been traditionally used for centuries as an analgesic and anti-inflammatory remedy also in different gastrointestinal disorders. This study aims to investigate the effects of different C. sativa isolated compounds in an in vitro model of intestinal epithelium. The ability of treatments to modulate markers of intestinal dysfunctions was tested on Caco-2 intestinal cell monolayers. Our results, obtained by evaluation of ROS production, TEER and paracellular permeability measurements and tight junctions evaluation show Cannabidiol as the most promising compound against intestinal inflammatory condition. Cannabidiol is able to inhibit ROS production and restore epithelial permeability during inflammatory and oxidative stress conditions, suggesting its possible application as adjuvant in IBD management.

Intestinal Cell Culture Models

2001 ◽  
pp. 97-113 ◽  
Author(s):  
Yula Sambruy ◽  
S. Ferruzza ◽  
G. Ranaldi ◽  
I. De Angelis
Keyword(s):  
Cell Culture ◽  
Intestinal Cell ◽  
Culture Models ◽  
Cell Culture Models

Specific biochemical features of replication of clinical influenza viruses in human intestinal cell culture

2007 ◽  
Vol 72 (4) ◽  
pp. 398-408 ◽  
Author(s):  
O. P. Zhirnov ◽  
I. V. Vorobjeva ◽  
O. A. Saphonova ◽  
N. A. Malyshev ◽  
A. V. Ovcharenko ◽  
...  
Keyword(s):  
Cell Culture ◽  
Influenza Viruses ◽  
Intestinal Cell ◽  
Biochemical Features

scholarly journals Challenges of Culturing Human Norovirus in Three-Dimensional Organoid Intestinal Cell Culture Models

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e63485 ◽  
Author(s):  
Efstathia Papafragkou ◽  
Joanne Hewitt ◽  
Geun Woo Park ◽  
Gail Greening ◽  
Jan Vinjé
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Intestinal Cell ◽  
Human Norovirus ◽  
Culture Models ◽  
Cell Culture Models

Intestinal cell culture models

1994 ◽  
Vol 2 (1-2) ◽  
pp. 36-38 ◽  
Author(s):  
C.L. Laboisse ◽  
A. Jarry ◽  
C. Bou-Hanna ◽  
D. Merlin ◽  
G. Vallette
Keyword(s):  
Cell Culture ◽  
Intestinal Cell ◽  
Culture Models ◽  
Cell Culture Models
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