Transcriptomic and Metabolic Analysis of Fruit Development and Identification of Genes Involved in Raffinose and Hydrolysable Tannin Biosynthesis in Walnuts

Author(s):  
Hua Wang ◽  
Keysarjan Asker ◽  
Chang Zhan ◽  
Nian Wang
2020 ◽  
Author(s):  
Zhen Chen

Abstract Background: Rubus chingii Hu (Chinese raspberry) is an important dual functional food with nutraceutical and pharmaceutical values. Comprehensive understanding of fruit development and bioactive components synthesis and regulation could accelerate genetic analysis and molecular breeding for the unique species.Results: A combined transcriptomic and metabolic analysis of R. chingii fruits from different developmental stages was conducted in this study. A total of 89,188 unigenes was generated and 57,545 unigenes (64.52%) were got annotated. Differential expression genes (DEGs) and differential ions mainly involved in biosynthesis of secondary metabolites. Phenolic acids and flavonol glycosides syntheses were strongly activated at earlier stages, while amino acids, linolenic acid metabolism and anthocyanins synthesis were dominant at later stages. The core genes participated in biosynthesis of ellagic acid (EA) and kaempferol-3-O-rutinoside (K-3-R) and their corresponding metabolites were elaborately characterized. And some probable MYB and bHLH transcription factors controlling flavonoids synthesis were also identified. Conclusion: Combined transcriptomic and metabolic analysis initially reveals molecular and chemical mechanism of fruit development of R. chingii Hu fruit. The fruit launched the biosynthesis of phenolic acids and flavonols at the very beginning of fruit set and then coordinately accumulated and converted. And it was tightly regulated by expressions of the related genes and transcription factors. The results provide a solid foundation for genetic analysis, functional genes isolation, fruit quality improvement and modifiable breeding.


2020 ◽  
Vol 149 ◽  
pp. 233-244 ◽  
Author(s):  
Michail Michailidis ◽  
Evangelos Karagiannis ◽  
Georgia Tanou ◽  
Martina Samiotaki ◽  
Eirini Sarrou ◽  
...  

1992 ◽  
Vol 85 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Maria-Jose Sanchez-Beltran ◽  
Juan Carbonell ◽  
Jose L. Garcia-Martinez ◽  
Isabel Lopez-Diaz

Fruits ◽  
2015 ◽  
Vol 70 (4) ◽  
pp. 201-212 ◽  
Author(s):  
Esther Aurora Ruiz Huerta ◽  
Judith Márquez Guzmán ◽  
Clara Pelayo Zaldívar ◽  
Claudia Barbosa Martínez ◽  
Leticia Ponce de León García

1997 ◽  
Vol 66 (2) ◽  
pp. 253-259 ◽  
Author(s):  
Shiping Wang ◽  
Goro Okamoto ◽  
Ken Hirano

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