Identification and Characterization of a New Quorum-Quenching N-acyl Homoserine Lactonase in the Plant Pathogen Erwinia amylovora

2021 ◽  
Author(s):  
Sapir Ya’ar Bar ◽  
Shlomit Dor ◽  
Mayan Erov ◽  
Livnat Afriat-Jurnou
Keyword(s):  
Plant Pathogen ◽  
Erwinia Amylovora ◽  
Quorum Quenching ◽  
Identification And Characterization

scholarly journals Identification and Characterization of Quorum-Quenching Activity of N-Acylhomoserine Lactonase from Coagulase-Negative Staphylococci

Antibiotics ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 483
Author(s):  
Tomohiro Morohoshi ◽  
Yaoki Kamimura ◽  
Nobutaka Someya
Keyword(s):  
Quorum Sensing ◽  
Opportunistic Pathogen ◽  
Quorum Quenching ◽  
Coagulase Negative Staphylococci ◽  
Sensing Systems ◽  
Genes Encoding ◽  
Acyl Chains ◽  
Gene Homologue ◽  
Identification And Characterization

N-Acylhomoserine lactones (AHLs) are used as quorum-sensing signals in Gram-negative bacteria. Many genes encoding AHL-degrading enzymes have been cloned and characterized in various microorganisms. Coagulase-negative staphylococci (CNS) are present on the skin of animals and are considered low-virulent species. The AHL-lactonase gene homologue, ahlS, was present in the genomes of the CNS strains Staphylococcus carnosus, Staphylococcus haemolyticus, Staphylococcus saprophyticus, and Staphylococcus sciuri. We cloned the candidate ahlS homologue from six CNS strains into the pBBR1MCS5 vector. AhlS from the CNS strains showed a higher degrading activity against AHLs with short acyl chains compared to those with long acyl chains. AhlS from S. sciuri was expressed and purified as a maltose-binding protein (MBP) fusion. Pseudomonas aeruginosa is an opportunistic pathogen that regulates several virulence factors such as elastase and pyocyanin by quorum-sensing systems. When MBP-AhlS was added to the culture of P. aeruginosa PAO1, pyocyanin production and elastase activity were substantially reduced compared to those in untreated PAO1. These results demonstrate that the AHL-degrading activity of AhlS from the CNS strains can inhibit quorum sensing in P. aeruginosa PAO1.

scholarly journals Identification and Characterization of theErwinia amylovora rpoS Gene: RpoS Is Not Involved in Induction of Fireblight Disease Symptoms

1998 ◽  
Vol 180 (24) ◽  
pp. 6789-6792 ◽  
Author(s):  
M. Anderson ◽  
C. E. Pollitt ◽  
I. S. Roberts ◽  
J. A. Eastgate
Keyword(s):  
Sigma Factor ◽  
Erwinia Amylovora ◽  
Environmental Stresses ◽  
Alternative Sigma Factor ◽  
Gene Encoding ◽  
Disease Symptoms ◽  
Highly Sensitive ◽  
Identification And Characterization ◽  
Sigma Factor Rpos

ABSTRACT The Erwinia amylovora rpoS gene, encoding the alternative sigma factor RpoS, has been cloned and characterized. Though highly sensitive to a number of environmental stresses, anE. amylovora rpoS mutant was not compromised in its ability to grow or cause disease symptoms within apple seedlings or in an overwintering model.

scholarly journals Characterization of acfr-Carrying Plasmid from Porcine Escherichia coli That Closely Resembles Plasmid pEA3 from the Plant Pathogen Erwinia amylovora

2015 ◽  
Vol 60 (1) ◽  
pp. 658-661 ◽  
Author(s):  
Rongmin Zhang ◽  
Bin Sun ◽  
Yang Wang ◽  
Lei Lei ◽  
Stefan Schwarz ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Dna Sequence ◽  
Plant Pathogen ◽  
Erwinia Amylovora ◽  
The Other ◽  
Plant Origin ◽  
Chromosomal Dna ◽  
Content Type

ABSTRACTThe multiresistance genecfrwas found in two porcineEscherichia coliisolates, one harboring it on the conjugative 33,885-bp plasmid pFSEC-01, the other harboring it in the chromosomal DNA. Sequence analysis of pFSEC-01 revealed that a 6,769-bp fragment containing thecfrgene bracketed by two IS26elements was inserted into a plasmid closely related to pEA3 from the plant pathogenErwinia amylovora, suggesting that pFSEC-01 may be transferred between different bacterial genera of both animal and plant origin.

Application of commercial test-systems to identify gram-negative facultatively anaerobic bacteria

2016 ◽  
Vol 3 (1) ◽  
pp. 43-48 ◽  
Author(s):  
V. Patyka ◽  
L. Butsenko ◽  
L. Pasichnyk
Keyword(s):  
Erwinia Amylovora ◽  
Anaerobic Bacteria ◽  
Biochemical Properties ◽  
Test System ◽  
Phytopathogenic Bacteria ◽  
Gram Negative ◽  
Test Systems ◽  
Facultatively Anaerobic ◽  
Microbiological Methods

Aim. To validate the suitability of commercial API 20E test-system (bioMerieux) for the identifi cation and characterization of facultative gram-negative phytopathogenic bacterial isolates. Methods. Conventional mi- crobiological methods, API 20E test-system (bioMerieux) according to the manufacturer’s instructions. Re- sults. The identifi cation results for Erwinia amylovora, Pectobacterium carotovorum and Pantoea agglome- rans isolates were derived from the conventional and API 20E test systems, which, were in line with the literature data for these species. The API 20E test-system showed high suitability for P. agglomerans isolates identifi cation. Although not all the species of facultatively anaerobic phytopathogenic bacteria may be identi- fi ed using API 20E test-system, its application will surely allow obtaining reliable data about their physiologi- cal and biochemical properties, valuable for identifi cation of bacteria, in the course of 24 h. Conclusions. The results of tests, obtained for investigated species while using API 20E test-system, and those of conventional microbiological methods coincided. The application of API 20E test-system (bioMerieux) ensures fast obtain- ing of important data, which may be used to identify phytopathogenic bacteria of Erwinia, Pectobacterium, Pantoea genera.

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