Dragonfly-Associated Trichoderma harzianum QTYC77 Is Not Only a Potential Biological Control Agent of Fusarium oxysporum f. sp. cucumerinum But Also a Source of New Antibacterial Agents

2020 ◽  
Vol 68 (48) ◽  
pp. 14161-14167
Author(s):  
Shuxiang Zhang ◽  
Feifei Sun ◽  
Lijun Liu ◽  
Liyun Bao ◽  
Wei Fang ◽  
...  
Keyword(s):  
Biological Control ◽  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Antibacterial Agents ◽  
Biological Control Agent ◽  
Control Agent

scholarly journals Seleksi Komposisi Medium Pertumbuhan dan Bahan Pembawa untuk Formulasi Cendawan Agens Hayati Fusarium oxysporum Non-Patogenik P21a

2019 ◽  
Vol 15 (2) ◽  
pp. 44-52
Author(s):  
Fany Juliarti Panjaitan ◽  
Suryo Wiyono ◽  
Rahayu Widyastuti
Keyword(s):  
Biological Control ◽  
Fusarium Oxysporum ◽  
Growth Medium ◽  
Storage Temperature ◽  
Biological Control Agent ◽  
Critical Factor ◽  
Control Agent ◽  
Mycelium Growth ◽  
And Storage ◽  
Selection Of

Selection of Compositions of Growth Medium and Carriers for Formulation of Biological Agents of Non-Pathogenic Fusarium oxysporum P21aThe potency of non-pathogenic Fusarium oxysporum P21a (NPFo P21a) as a biological control agent has prospect to be developed commercially. The growth medium and carrier are the critical factor to formulate biological control. This study aimed to obtain the best solid medium dan lighting type for mycelium growth and sporulation as well as to determine the carrier and storage temperature which is suitable for NPFo P21a toward the survival of propagule and germination of shallot. The results showed that M2 medium treatment (rice grain:rice bran; 20:1 w/w) was the best medium for mycelium growth. The M3 medium treatment (20:2 w/w) was the best medium for sporulation of NPFo P21a. The near-UV lighting treatment was able to stimulate the macroconidium production of NPFo P21a. Talc-based formulation and the storage at 20 °C showed the best shelf-life for NPFo P21a with density of viable propagule and shallot germination better.

scholarly journals EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG

2017 ◽  
Vol 16 (2) ◽  
pp. 115
Author(s):  
Johanna Taribuka ◽  
Christanti Sumardiyono ◽  
Siti Muslimah Widyastuti ◽  
Arif Wibowo
Keyword(s):  
Biological Control ◽  
Phylogenetic Analysis ◽  
Endophytic Fungi ◽  
Trichoderma Harzianum ◽  
Biocontrol Agent ◽  
Biological Control Agent ◽  
Control Agent ◽  
Selective Medium ◽  
Trichoderma Spp ◽  
Its1 And Its2

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.

scholarly journals Pellet Formulation of Trichoderma Isolated from Aceh with Potential for Biocontrol of Phytophthora Sp. on Cacao Sedlings

2019 ◽  
Vol 3 (2) ◽  
pp. 202-212
Author(s):  
Rina Sriwati ◽  
Tjut Chamzhurni ◽  
Alfizar Alfizar ◽  
Bonny PW Soekarno ◽  
Vina Maulidia ◽  
...  
Keyword(s):  
Biological Control ◽  
Disease Severity ◽  
Trichoderma Harzianum ◽  
Biological Control Agent ◽  
Control Agent ◽  
Diameter Growth ◽  
Good Ability ◽  
Trichoderma Isolate ◽  
Pellet Formulation

Molleculler study was conducted to identify several species of Trichoderma isolate from several  plant (Pine, Cacao, Gliceria, Nutmeg, Bamboo, Coffee, Potato).  The growth of eight species Trichodermaafter pelleting formulation has been observed. Pellet Trichoderma harzianum have good ability to growth on PDA medium after 4 weeks storage. Base on their mycelium diameter growth on PDA, T. harzianum have selected as potential species on pellet formulation growth.  Several dose of pellet formulation have been applied for controlling Phythopthora disease. The application of T. harzianum pellets in the form of a 2 g / 100 ml (S1) suspension effective in inhibiting the development of Phytophthora sp in cacao seedlings, when the higher concentrations of T. harzianum pellets applied to cacao seeds,the disease severity increase. Pellet Trichoderma could be use as biological control agent of cacao seedling in certain dosage.

scholarly journals Cladobotryum mycophilum as Potential Biocontrol Agent

Agronomy ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 891
Author(s):  
Mila Santos ◽  
Fernando Diánez ◽  
Alejandro Moreno-Gavíra ◽  
Brenda Sánchez-Montesinos ◽  
Francisco J. Gea
Keyword(s):  
Biological Control ◽  
Fusarium Oxysporum ◽  
Biocontrol Agent ◽  
Biological Control Agent ◽  
Control Agent ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Dual Culture ◽  
Potential Biocontrol Agent

A study was conducted to explore the efficacy of potential biocontrol agent Cladobotryum mycophilum against different phytopathogenic fungi. The growth rates of 24 isolates of C. mycophilum were determined, and their antagonistic activity was analysed in vitro and in vivo against Botrytis cinerea, Fusarium oxysporum f. sp. radicis-lycopersici, Fusarium oxysporum f.sp. cucumerinum, Fusarium solani, Phytophthora parasitica, Phytophthora capsici, Pythium aphanidermatum and Mycosphaerella melonis. Most isolates grow rapidly, reaching the opposite end of the Petri dish within 72–96 h. Under dual-culture assays, C. mycophilum showed antagonistic activity in vitro against all phytopathogenic fungi tested, with mycelial growth inhibition ranging from 30 to 90% against all the different phytopathogens tested. Similarly, of all the selected isolates, CL60A, CL17A and CL18A significantly (p < 0.05) reduced the disease incidence and severity in the plant assays compared to the controls for the different pathosystems studied. Based on these results, we conclude that C. mycophilum can be considered as a potential biological control agent in agriculture. This is the first study of Cladobotryum mycophilum as a biological control agent for different diseases caused by highly relevant phytopathogens in horticulture.

scholarly journals Rhizosphere Streptomyces formulas as the biological control agent of phytopathogenic fungi Fusarium oxysporum and plant growth promoter of soybean

2021 ◽  
Vol 22 (6) ◽  
Author(s):  
MAYA SARI ◽  
ABDJAD ASIH NAWANGSIH ◽  
Aris Tri Wahyudi
Keyword(s):  
Biological Control ◽  
Plant Growth ◽  
Fusarium Oxysporum ◽  
Biological Control Agent ◽  
Control Agent ◽  
Phytopathogenic Fungi ◽  
Disease Suppression ◽  
Growth Promoter ◽  
Sterile Soil ◽  
Plant Growth Promoter

Abstract. Sari M, Nawangsih AA, Wahyudi AT. 2021. Rhizosphere Streptomyces formulas as the biological control agent of phytopathogenic fungi Fusarium oxysporum and plant growth promoter of soybean. Biodiversitas 22: 3015-3023. Rhizosphere Streptomyces are considered as promising sources of plant growth-promoting rhizobacteria (PGPR) and biocontrol agents against pathogenic fungi, particularly Fusarium oxysporum causing root rot, cotyledon rot, hypocotyl rot, and stunted growth in soybean. Formulation of rhizosphere Streptomyces with appropriate carrier materials is necessary to facilitate storage and application in plants. This study aimed to develop a formulation of rhizosphere Streptomyces, apply the formula to control F. oxysporum, and promote soybean plant growth. Five Streptomyces isolates, i.e., Streptomyces panaciradicis ARK 13, Streptomyces tritolerans ARK 17, Streptomyces recifensis ARK 63, Streptomyces tendae ARK 91, and Streptomyces manipurensis ARK 94 were used in this study. All of the isolates could grow in potato broth, rice bran extract, and molasses as alternative media. The highest biomass produced from the molasses growth medium. All five isolates had antifungal activity against F. oxysporum with the inhibition percentage ranging from 41% to 76%, and all of them were detected to have the iaaM gene. Indole-3-acetic acid (IAA) hormone produced by these isolates were ranging from 8.99-15.14 mg L-1, with the phosphate solubilization index of 2.13-2.47. Five rhizosphere Streptomyces formulas with the main carrier of peat could maintain the viability with the population density of 108 CFU g-1 for 8 weeks of storage at room temperature. Two formulas, F17 and F94, were the best formulas to control disease caused by F. oxysporum with disease suppression of 74% in sterile soil and 80-85% in non-sterile soil. Formula F17 and F94 significantly increased soybean growth in sterile and non-sterile soils. Therefore, these formulas could be recommended as biocontrol and plant growth promoters of soybean.

qRT-PCR quantification of the biological control agent Trichoderma harzianum in peat and compost-based growing media

2011 ◽  
Vol 102 (3) ◽  
pp. 2793-2798 ◽  
Author(s):  
Robert Beaulieu ◽  
Rubén López-Mondéjar ◽  
Fabio Tittarelli ◽  
Margarita Ros ◽  
José Antonio Pascual
Keyword(s):  
Biological Control ◽  
Trichoderma Harzianum ◽  
Biological Control Agent ◽  
Control Agent ◽  
Growing Media ◽  
Qrt Pcr

Evaluation of Trichoderma harzianum strain T22 as biological control agent of Calonectria pauciramosa

BioControl ◽  
2011 ◽  
Vol 57 (5) ◽  
pp. 687-696 ◽  
Author(s):  
Alessandro Vitale ◽  
Gabriella Cirvilleri ◽  
Ivana Castello ◽  
Dalia Aiello ◽  
Giancarlo Polizzi
Keyword(s):  
Biological Control ◽  
Trichoderma Harzianum ◽  
Biological Control Agent ◽  
Control Agent

scholarly journals ANTAGONISTIC POTENTIAL OF TRICHODERMA HARZIANUM AGAINST FUSARIUM OXYSPORUM F. SP. CUBENSE ASSOCIATED WITH PANAMA WILT OF BANANA

2017 ◽  
Vol 29 (1) ◽  
pp. 111
Author(s):  
Babar Khan ◽  
Zuniara Akash ◽  
Shahzad Asad ◽  
Nazir Javed ◽  
Nasir A. Rajput ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Vascular System ◽  
Biological Control Agent ◽  
Colony Growth ◽  
Research Area ◽  
Control Agent ◽  
Research Centre ◽  
Antagonistic Potential

Fusarium oxysporum f. sp. cubense is a soil loving pathogen of banana that can cause distortion of vascular system. It is responsible for the disease Fusarium wilt of bananas also renowned as panama wilt disease that has responsible of immense losses in the banana industry worldwide. In this study, diseased samples were collected from rhizosphere of banana plants in the research area of National Agriculture Research Centre (NARC), Islamabad and cultured on PDA to isolate pathogenic strains of F. oxysporium f. sp. cubense. Antagonistic potential of Trichoderma harzianum against F. oxysporum f. sp. cubense was evaluated under controlled conditions. T. harzianum produced up to 75.5% inhibition of colony growth of the pathogen followed by incubation for 72 h at 28+2°C in vitro. In pot culture T. harzianum considerably reduced disease severity. It proves that it is a potential biological control agent against banana wilt pathogen.

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