Optimization of Culture Conditions for the Efficient Biosynthesis of Trilobatin from Phloretin by Engineered Escherichia coli Harboring the Apple Phloretin-4′-O-glycosyltransferase

2020 ◽  
Vol 68 (48) ◽  
pp. 14212-14220
Author(s):  
Bhagwat Nawade ◽  
Mosaab Yahyaa ◽  
Rachel Davidovich-Rikanati ◽  
Efraim Lewinsohn ◽  
Mwafaq Ibdah
Keyword(s):  
Escherichia Coli ◽  
Culture Conditions ◽  
Optimization Of Culture Conditions ◽  
Engineered Escherichia Coli

Expression and Secretion of Endostar Protein by Escherichia Coli: Optimization of Culture Conditions Using the Response Surface Methodology

2016 ◽  
Vol 58 (10) ◽  
pp. 634-647 ◽  
Author(s):  
Abbas Mohajeri ◽  
Jalal Abdolalizadeh ◽  
Younes Pilehvar-Soltanahmadi ◽  
Farhad Kiafar ◽  
Nosratollah Zarghami
Keyword(s):  
Escherichia Coli ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Culture Conditions ◽  
Optimization Of Culture Conditions

Improved l-threonine production of Escherichia coli mutant by optimization of culture conditions

2006 ◽  
Vol 101 (2) ◽  
pp. 127-130 ◽  
Author(s):  
Man-Hyo Lee ◽  
Hong-Weon Lee ◽  
Jin-Ho Park ◽  
Jung-Oh Ahn ◽  
Joon-Ki Jung ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Culture Conditions ◽  
Coli Mutant ◽  
Threonine Production ◽  
Optimization Of Culture Conditions

Biodecomposition of Phenanthrene and Pyrene by a Genetically Engineered Escherichia coli

2020 ◽  
Vol 14 (2) ◽  
pp. 121-133 ◽  
Author(s):  
Maryam Ahankoub ◽  
Gashtasb Mardani ◽  
Payam Ghasemi-Dehkordi ◽  
Ameneh Mehri-Ghahfarrokhi ◽  
Abbas Doosti ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Performance ◽  
Human Cancer ◽  
Genetically Engineered ◽  
Hazardous Substances ◽  
E Coli ◽  
Spiked Soil ◽  
Engineered Escherichia Coli ◽  
Genetically Manipulated ◽  
Hplc Measurement

Background: Genetically engineered microorganisms (GEMs) can be used for bioremediation of the biological pollutants into nonhazardous or less-hazardous substances, at lower cost. Polycyclic aromatic hydrocarbons (PAHs) are one of these contaminants that associated with a risk of human cancer development. Genetically engineered E. coli that encoded catechol 2,3- dioxygenase (C230) was created and investigated its ability to biodecomposition of phenanthrene and pyrene in spiked soil using high-performance liquid chromatography (HPLC) measurement. We revised patents documents relating to the use of GEMs for bioremediation. This approach have already been done in others studies although using other genes codifying for same catechol degradation approach. Objective: In this study, we investigated biodecomposition of phenanthrene and pyrene by a genetically engineered Escherichia coli. Methods: Briefly, following the cloning of C230 gene (nahH) into pUC18 vector and transformation into E. coli Top10F, the complementary tests, including catalase, oxidase and PCR were used as on isolated bacteria from spiked soil. Results: The results of HPLC measurement showed that in spiked soil containing engineered E. coli, biodegradation of phenanthrene and pyrene comparing to autoclaved soil that inoculated by wild type of E. coli and normal soil group with natural microbial flora, were statistically significant (p<0.05). Moreover, catalase test was positive while the oxidase tests were negative. Conclusion: These findings indicated that genetically manipulated E. coli can provide an effective clean-up process on PAH compounds and it is useful for bioremediation of environmental pollution with petrochemical products.

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