Novel Insights into Phosphorus Deprivation Boosted Lipid Synthesis in the Marine Alga Nannochloropsis oceanica without Compromising Biomass Production

2020 ◽  
Vol 68 (41) ◽  
pp. 11488-11502
Author(s):  
Ying Shi ◽  
Meijing Liu ◽  
Wei Ding ◽  
Jin Liu
Keyword(s):  
Biomass Production ◽  
Marine Alga ◽  
Lipid Synthesis ◽  
Nannochloropsis Oceanica ◽  
Phosphorus Deprivation

scholarly journals A Study on the Effect of Macro- and Micro- Nutrients on Nannochloropsis oceanica Growth, Fatty Acid Composition and Magnetic Harvesting Efficiency

Plants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 660 ◽  
Author(s):  
Maria G. Savvidou ◽  
Elenitsa Boli ◽  
Dimitrios Logothetis ◽  
Theopisti Lymperopoulou ◽  
Angelo Ferraro ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Lipid Synthesis ◽  
Monounsaturated Fatty Acids ◽  
Culture Conditions ◽  
Nannochloropsis Oceanica ◽  
Fatty Acid Production ◽  
Electrostatic Charges ◽  
Phosphorus Deprivation ◽  
Harvesting Efficiency

The effect of iron, manganese, phosphorus and nitrogen on growth and lipid synthesis of the microalgae Nannochloropsis oceanica CCMP1779, as well as their impact on the magnetic harvesting efficiency, are examined under their depriving cell culture conditions. Herein, it is demonstrated that nitrogen and manganese depletion primarily reduced cell growth while phosphorus and iron restriction led to higher dry biomass. Subsequently, the role of those nutrients on fatty acids profile was examined. Phosphorus and nitrogen restriction resulted in lower and higher lipid content, respectively. High amounts of polyunsaturated fatty acids like eicosapentaenoic acid are produced under iron and manganese depletion. Phosphorus deprivation favors monounsaturated fatty acids such as C18:1 and C16:1, while nitrogen restriction favors saturated fatty acid production like C14:0, C16:0 and C18:0. Since the presence/absence of macro- and micro-elements may affect the overall electrostatic charges on the outmost microalgae surface, it was also analyzed how these elements affect the magnetic harvesting efficiency. Results showed that phosphorus deprivation led to the best magnetic harvesting efficiency of N. oceanica cells (93%) as compared to other nutrient starvation as well as standard medium.

scholarly journals Enhancing oil production and harvest by combining the marine alga Nannochloropsis oceanica and the oleaginous fungus Mortierella elongata

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhi-Yan Du ◽  
Jonathan Alvaro ◽  
Brennan Hyden ◽  
Krzysztof Zienkiewicz ◽  
Nils Benning ◽  
...  
Keyword(s):  
Marine Alga ◽  
Oil Production ◽  
Nannochloropsis Oceanica ◽  
Oleaginous Fungus ◽  
Mortierella Elongata

scholarly journals Integration of proteome and transcriptome refines key molecular processes underlying oil production in Nannochloropsis oceanica

2019 ◽  
Author(s):  
Wuxin You ◽  
Li Wei ◽  
Yanhai Gong ◽  
Mohamed El Hajjami ◽  
Jian Xu ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Nitrogen Deficiency ◽  
Lipid Synthesis ◽  
Proteome Analysis ◽  
Tca Cycle ◽  
Membrane Lipid ◽  
Nannochloropsis Oceanica ◽  
Kennedy Pathway ◽  
The Tca Cycle ◽  
Post Transcriptional Regulation

Abstract Background Under nitrogen deficiency situation, Nannochloropsis spp. accumulate large amounts of lipid in the form of triacylglycerides (TAG). Several researches have studied the mechanism of this process from the perspective of transcriptome and metabolome, yet proteome analysis on this process is still sparse and lacking the analysis of the dynamic adaption to nitrogen deficiency. Here, proteomes for 03h, 06h, 12h, 24h, 48h and 10th day of nitrogen deplete and replete conditions were compared. New proteome results were integrated with existing transcriptome and other data.Results Obtained results illuminated physiological adaptations not deduced from previous transcriptome data: (a) Abundance of proteins related to photosynthesis only slightly decreased in the first 48h, indicating that photosynthesis is still working efficiently, and protein amounts adjust gradually with reduction in chloroplast size. (b) Most proteins related to the TCA cycle were strongly upregulated after 48h under nitrogen deficiency, suggesting that respiration is enhanced after 48h and that TCA cycle efflux supports the carbon required for lipid synthesis. (c) Proteins related to lipid accumulation via the Kennedy pathway increased their abundance at 48h, synchronous with the previously reported diversification of fatty acids after 48h.Conclusions This study adds a proteome perspective on the major pathways for TAG accumulation by Nannochloropsis due to absence of nitrogen: photosynthesis, membrane lipid conversion, protein degradation, TCA cycle. By integrating existing transcriptome and other data, our research provided for Nannochloropsis oceanica a multi-layered description of adaptation to nitrogen limitation and lipid accumulation. Cluster analysis of this integrated dataset allowed inference of post-transcriptional regulation events.

scholarly journals Integration of proteome and transcriptome refines key molecular processes underlying oil production in Nannochloropsis oceanica

2020 ◽  
Author(s):  
Wuxin You ◽  
Li Wei ◽  
Yanhai Gong ◽  
Mohamed El Hajjami ◽  
Jian Xu ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Nitrogen Deficiency ◽  
Lipid Synthesis ◽  
Proteome Analysis ◽  
Tca Cycle ◽  
Membrane Lipid ◽  
Nannochloropsis Oceanica ◽  
Kennedy Pathway ◽  
The Tca Cycle ◽  
Post Transcriptional Regulation

Abstract Background: Under nitrogen deficiency situation, Nannochloropsis spp. accumulate large amounts of lipid in the form of triacylglycerides (TAG). Several researches have studied the mechanism of this process from the perspective of transcriptome and metabolome, yet proteome analysis on this process is still sparse and lacking the analysis of the dynamic adaption to nitrogen deficiency. Here, proteomes for 03h, 06h, 12h, 24h, 48h and 10th day of nitrogen deplete and replete conditions were compared. New proteome results were integrated with existing transcriptome and other data. Results: Obtained results illuminated physiological adaptations not deduced from previous transcriptome data: (a) Abundance of proteins related to photosynthesis only slightly decreased in the first 48h, indicating that photosynthesis is still working efficiently, and protein amounts adjust gradually with reduction in chloroplast size. (b) Most proteins related to the TCA cycle were strongly upregulated after 48h under nitrogen deficiency, suggesting that respiration is enhanced after 48h and that TCA cycle efflux supports the carbon required for lipid synthesis. (c) Proteins related to lipid accumulation via the Kennedy pathway increased their abundance at 48h, synchronous with the previously reported diversification of fatty acids after 48h. Conclusions: This study adds a proteome perspective on the major pathways for TAG accumulation by Nannochloropsis due to absence of nitrogen: photosynthesis, membrane lipid conversion, protein degradation, TCA cycle. By integrating existing transcriptome and other data, our research provided for Nannochloropsis oceanica a multi-layered description of adaptation to nitrogen limitation and lipid accumulation. Cluster analysis of this integrated dataset allowed inference of post-transcriptional regulation events.

Green integrated process for mitigation of municipal and industrial liquid and solid waste mixes for enhanced microalgal biomass and lipid synthesis for biodiesel

RSC Advances ◽  
2015 ◽  
Vol 5 (87) ◽  
pp. 70929-70938 ◽  
Author(s):  
Ankush Karemore ◽  
Ramkrishna Sen
Keyword(s):  
Solid Waste ◽  
Biomass Production ◽  
Lipid Synthesis ◽  
Integrated Process ◽  
Microalgal Biomass ◽  
Biodiesel Feedstock

We herein report: a sustainable green integrated process for effective waste mitigation and microalgal biomass production for biodiesel feedstock generation in a biorefinery model.

Fine Structural Localization of Acyltransferases Involved in Lipid Synthesis in Intestinal Mucosa.

1970 ◽  
Vol 28 ◽  
pp. 54-55
Author(s):  
R. J. Barrnett ◽  
J. A. Higgins
Keyword(s):  
Smooth Endoplasmic Reticulum ◽  
Lipid Synthesis ◽  
Mucosal Cell ◽  
Structural Localization ◽  
Morphological Studies ◽  
Mucosal Cells ◽  
Initial Appearance ◽  
Sh Group ◽  
Hydrolysis Of ◽  
Intestinal Mucosal Cells

The main products of intestinal hydrolysis of dietary triglycerides are free fatty acids and monoglycerides. These form micelles from which the lipids are absorbed across the mucosal cell brush border. Biochemical studies have indicated that intestinal mucosal cells possess a triglyceride synthesising system, which uses monoglyceride directly as an acylacceptor as well as the system found in other tissues in which alphaglycerophosphate is the acylacceptor. The former pathway is used preferentially for the resynthesis of triglyceride from absorbed lipid, while the latter is used mainly for phospholipid synthesis. Both lipids are incorporated into chylomicrons. Morphological studies have shown that during fat absorption there is an initial appearance of fat droplets within the cisternae of the smooth endoplasmic reticulum and that these subsequently accumulate in the golgi elements from which they are released at the lateral borders of the cell as chylomicrons.We have recently developed several methods for the fine structural localization of acyltransferases dependent on the precipitation, in an electron dense form, of CoA released during the transfer of the acyl group to an acceptor, and have now applied these methods to a study of the fine structural localization of the enzymes involved in chylomicron lipid biosynthesis. These methods are based on the reduction of ferricyanide ions by the free SH group of CoA.

Optimization of biomass production with enhanced bioactive compound content by the medicinal mushroom Ganoderma australe under submerged culture

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
LM Papaspyridi ◽  
E Topakas ◽  
N Aligiannis ◽  
P Christakopoulos ◽  
AL Skaltsounis ◽  
...  
Keyword(s):  
Biomass Production ◽  
Submerged Culture ◽  
Bioactive Compound ◽  
Medicinal Mushroom ◽  
Ganoderma Australe
Sign in / Sign up

Export Citation Format

Share Document