Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis

2020 ◽  
Vol 68 (44) ◽  
pp. 12295-12309
Author(s):  
Xin Shao ◽  
Chongzhen Sun ◽  
Xin Tang ◽  
Xiaosa Zhang ◽  
Duo Han ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Sodium Sulfate ◽  
Allium Sativum ◽  
Dextran Sodium Sulfate ◽  
Anti Inflammatory

scholarly journals THE EFFECT OF MAHKOTA DEWA (PHALERIA MACROCARPA) (SCHEFF.) FRUIT PERICARP EXTRACT ON INOS IN MICE COLON INTERMITTENTLY-INDUCED BY DEXTRAN SODIUM SULFATE

2017 ◽  
Vol 10 (12) ◽  
pp. 309
Author(s):  
Kusmardi Kusmardi ◽  
Dilla Shavera ◽  
Ari Estuningtyas ◽  
Aryo Tedjo ◽  
Bambang Priyosoeryanto
Keyword(s):  
Sodium Sulfate ◽  
Total Yield ◽  
Ethanolic Extract ◽  
Dextran Sodium Sulfate ◽  
Inflammatory Activity ◽  
Fruit Pericarp ◽  
Phaleria Macrocarpa ◽  
Anti Inflammatory ◽  
Oxide Synthase ◽  
Different Doses

  Objective: The objective of this research was to investigate the anti-inflammatory effect of Mahkota Dewa fruit pericarp extract (Phaleria macrocarpa) on inducible nitric oxide synthase (iNOS) in mice colon induced by dextran sodium sulfate (DSS).Method: The simplisia of P. macrocarpa pericarp was weighed (1000 g) and extracted by maceration process. The total yield of the ethanolic extract was 26.43%. Phytochemical screening was carried out for the detection of the phytoconstituents by simple qualitative methods. The anti-inflammatory activity was performed by DSS-induced colitis model through assessment of hematoxylin-eosin staining and expression of iNOS by immunohistochemistry assay at four different doses, i.e., 650, 1250, 2500, and 5000 mg/kg. Swiss Webster male mice weighing 25-30 g were used for the study.Results: Inflammation score in dose 625, 1250, 2500, and 5000 mg/kg were 1.63, 1.43, 1.32, and 2.20, respectively. This result is significantly different (p=0.008) with DSS group that was 4.37. The results of iNOS optical density score in dose 625, 1250, 2500, and 5000 mg/kg were 1.21, 1.119, 1.22, and 1.37, respectively. This result was significantly different (p=0.000) with DSS group that was 2.24.Conclusion: Pericarp extract of P. macrocarpa fruit exhibited anti-inflammatory activity in the experimental model shown by suppressing the expression of inflammatory cell and iNOS. 

scholarly journals Acetylsalicylic Acid Reduces the Severity of Dextran Sodium Sulfate-Induced Colitis and Increases the Formation of Anti-Inflammatory Lipid Mediators

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Thomas Köhnke ◽  
Beate Gomolka ◽  
Süleyman Bilal ◽  
Xiangzhi Zhou ◽  
Yanping Sun ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Acetylsalicylic Acid ◽  
Bowel Disease ◽  
Sodium Sulfate ◽  
Lipid Mediators ◽  
Dextran Sodium Sulfate ◽  
Cyclooxygenase Inhibitors ◽  
Omega 3 ◽  
Inflammatory Bowel ◽  
Anti Inflammatory

The role of non-steroidal anti-inflammatory drugs in inflammatory bowel disease is controversial, as they have been implicated in disease aggravation. Different from other cyclooxygenase inhibitors, acetylsalicylic acid (ASA) enhances the formation of anti-inflammatory and proresolution lipoxins derived from arachidonic acid as well as resolvins from omega-3 polyunsaturated fatty acids such as docosahexaenoic acid (DHA). In this study, we examined the effect of ASA on murine dextran sodium sulfate colitis. A mouse magnetic resonance imaging (MRI) protocol and post mortem assessment were used to assess disease severity, and lipid metabolites were measured using liquid chromatography-coupled tandem mass spectrometry. Decreased colitis activity was demonstrated by phenotype and MRI assessment in mice treated with ASA, and confirmed in postmortem analysis. Analysis of lipid mediators showed sustained formation of lipoxin A4 and an increase of DHA-derived 17-hydroxydocosahexaenoic acid (17-HDHA) after treatment with ASA. Furthermore,in vitroexperiments in RAW264.7 murine macrophages demonstrated significantly increased phagocytosis activity after incubation with 17-HDHA, supporting its proresolution effect. These results show a protective effect of ASA in a murine colitis model and could give a rationale for a careful reassessment of ASA therapy in patients with inflammatory bowel disease and particularly ulcerative colitis, possibly combined with DHA supplementation.

scholarly journals Melatonin Attenuates Dextran Sodium Sulfate Induced Colitis in Obese Mice

2021 ◽  
Vol 14 (8) ◽  
pp. 822
Author(s):  
Shijia Pan ◽  
Fan Hong ◽  
Letong Li ◽  
Yuan Guo ◽  
Xiaoxiao Qiao ◽  
...  
Keyword(s):  
Sodium Sulfate ◽  
Antioxidant Activities ◽  
Intestinal Barrier ◽  
Inflammatory Cells ◽  
Inhibitory Effect ◽  
Immune Defense ◽  
Dextran Sodium Sulfate ◽  
Obese Mice ◽  
Promoting Effect ◽  
Anti Inflammatory

Epidemiological studies have indicated that obesity is an independent risk factor for colitis and that a high-fat diet (HFD) increases the deterioration of colitis-related indicators in mice. Melatonin has multiple anti-inflammatory effects, including inhibiting tumor growth and regulating immune defense. However, the mechanism of its activity in ameliorating obesity-promoted colitis is still unclear. This study explored the possibility that melatonin has beneficial functions in HFD-induced dextran sodium sulfate (DSS)-induced colitis in mice. Here, we revealed that HFD-promoted obesity accelerated DSS-induced colitis, while melatonin intervention improved colitis. Melatonin significantly alleviated inflammation by increasing anti-inflammatory cytokine release and reducing the levels of proinflammatory cytokines in HFD- and DSS-treated mice. Furthermore, melatonin expressed antioxidant activities and reversed intestinal barrier integrity, resulting in improved colitis in DSS-treated obese mice. We also found that melatonin could reduce the ability of inflammatory cells to utilize fatty acids and decrease the growth-promoting effect of lipids by inhibiting autophagy. Taken together, our study indicates that the inhibitory effect of melatonin on autophagy weakens the lipid-mediated prosurvival advantage, which suggests that melatonin-targeted autophagy may provide an opportunity to prevent colitis in obese individuals.

scholarly journals Anti-inflammatory effect of Perilla frutescens seed oil rich in omega-3 fatty acid on dextran sodium sulfate-induced colitis in mice

2021 ◽  
Vol 16 (5) ◽  
pp. 464 ◽  
Author(s):  
Napapan Kangwan ◽  
Komsak Pintha ◽  
Chakkrit Khanaree ◽  
Sarawut Kongkarnka ◽  
Teera Chewonarin ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Sodium Sulfate ◽  
Seed Oil ◽  
Perilla Frutescens ◽  
Dextran Sodium Sulfate ◽  
Omega 3 ◽  
Omega 3 Fatty Acid ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Salvia miltiorrhiza Ameliorates Disease Progression in Dextran-Sodium-Sulfate Induced Colitis in Mice

2021 ◽  
Author(s):  
Juan Chen ◽  
Danyu Chen ◽  
Lu Cheng ◽  
YongKang Yang ◽  
Weidong Gu ◽  
...  
Keyword(s):  
Sodium Sulfate ◽  
Activity Index ◽  
Salvia Miltiorrhiza ◽  
Disease Activity Index ◽  
Cerebrovascular Diseases ◽  
Dextran Sodium Sulfate ◽  
Inflammatory Factors ◽  
Inflammatory Bowel ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Abstract Salvia miltiorrhiza (SM, or Danshen) extract has been approved by China FDA for the treatment of cardiovascular and cerebrovascular diseases owing to its potent anti-inflammatory effects. Whether SM may be used to treat inflammatory bowel disease (IBD) remains elusive. In the current study, Dextran-Sodium-Sulfate (DSS) induced colitis in mice was used as a model of IBD, and SM was given orally for 7 days. SM administration has significantly reduced the disease activity index (DAI) score and weight lost and colon shortening in the DSS-induced colitis mice. The macrophage infiltration was significantly reduced in the SM treatment group. To explore the mechanisms, macrophage processor cell line Raw 264.7 was used to verify the anti-inflammatory effect of SM. SM treatment inhibited lipopolysaccharide (LPS)-induced macrophage activation in RAW264.7 cells and significantly reduced the production of pro-inflammatory factors. The current study provided evidence that oral administration of SM ameliorates pathological deterioration of IBD in mice, and warrants future clinical application of SM for the management of IBD.

scholarly journals Anti-Inflammatory Effects of Prunus mume Mixture in Colitis Induced by Dextran Sodium Sulfate

2011 ◽  
Vol 19 (1) ◽  
pp. 16-23 ◽  
Author(s):  
Hai-Lan Jin ◽  
Bo-Ram Lee ◽  
Kyung-Jik Lim ◽  
Trishna Debnath ◽  
Heung-Mook Shin ◽  
...  
Keyword(s):  
Sodium Sulfate ◽  
Dextran Sodium Sulfate ◽  
Prunus Mume ◽  
Anti Inflammatory

scholarly journals THE POTENCY OF INDONESIA’S POMEGRANATE PEEL ETHANOL EXTRACT (PUNICA GRANATUM LINN.) AS ANTI-INFLAMMATORY AGENT IN MICE COLON INDUCED BY DEXTRAN SODIUM SULFATE: FOCUS ON CYCLOOXYGENASE-2 AND INOS EXPRESSIONS

2017 ◽  
Vol 10 (12) ◽  
pp. 370 ◽  
Author(s):  
Kusmardi Kusmardi ◽  
Dony Hermanto ◽  
Ari Estuningytas ◽  
Aryo Tedjo ◽  
Bambang P Priosoeryanto
Keyword(s):  
Drinking Water ◽  
Ellagic Acid ◽  
Sodium Sulfate ◽  
Ethanol Extract ◽  
Dextran Sodium Sulfate ◽  
Pomegranate Peel ◽  
Inflammatory Agent ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Inflammation Score

Objective: Inflammatory disease occurs in the mucosal of the colon, or ulcerative colitis (UC) is one of subtypes of inflammatory bowel disease. The numerous of drug side effects for treatment of colitis give rise to use medicinal herbs as alternative therapies. Pomegranate peel extract has been used for the treatment of pain and inflammatory conditions. This study aimed to investigate the anti-inflammatory effects of pomegranate peel ethanol extract on mice colon through inflammation pathway which reduce inflammation score in mice model of chronic inflammation induced by dextran sodium sulfate (DSS).Methods: Thirty Swiss Webster mice divided randomly into 6 groups: Normal, aspirin 43 mg/kg/d (ASP), ellagic acid 26 mg/kg/d (ELG), DSS 2%b/v (DSS), pomegranate peel ethanol extract 240 mg/kg/d (DOSES-1), and 480 mg/kg/d (DOSES-2). All groups were given DSS 2% over 3 cycles except normal group (where each cycle in the DSS group consisted of 2% DSS in drinking water for 7 days, followed by a 7-day interval with normal drinking water). At the end of the experiment, colon samples were washed with water then buffered neutral formalin 10% fixed and paraffin embedded for histological analysis.Results: DOSES-1 and DOSES-2 were significantly reduced inflammation score in colon mice induced by DSS (p<0.05), mean score 2.01 and 2.02. Expression of cyclooxygenase (COX-2) was significantly decreased (p<0.05), mean score 27.48 and 17.77. Expression of iNOS was also significantly decreased (p<0.05), mean score 54.01 and 36.69. There were no significant differences between DOSES-1 and DOSES-2 groups with ASP and ELG group (p>0.05).Conclusion: Pomegranate peel ethanol extract has an anti-inflammatory agent by reduces inflammation score, inhibiting COX-2 and iNOS expression on mice colon by DSS induced. Furthermore, pomegranate peel ethanol extract has equivalent effectiveness with aspirin and pure ellagic acid.

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