Development of Real-Time Polymerase Chain Reaction Systems for the Detection of So-Called “Superfoods” Chia and Quinoa in Commercial Food Products

2020 ◽  
Vol 68 (49) ◽  
pp. 14334-14342 ◽  
Author(s):  
Hans-Volker Tichy ◽  
Anika Bruhs ◽  
Anja Palisch
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Food Products ◽  
Chain Reaction ◽  
Reaction Systems ◽  
Polymerase Chain

Quantitative real-time polymerase chain reaction for the presence of genetically-modified maize in breaded “ready-to-cook” food products

Food Control ◽  
2008 ◽  
Vol 19 (10) ◽  
pp. 1002-1005 ◽  
Author(s):  
Angela Di Pinto ◽  
Flora Alfano ◽  
Angela Giordano ◽  
Federico Capuano ◽  
Terio Valentina ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Genetically Modified ◽  
Food Products ◽  
Genetically Modified Maize ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Animal DNA identification in food products and animal feed by real time polymerase chain reaction method

ScienceRise ◽  
2016 ◽  
Vol 11 (0) ◽  
pp. 6-11
Author(s):  
Людмила Мар’янівна Іщенко ◽  
Лариса Миколаївна Шинкаренко ◽  
Ілля Володимирович Андрєєв ◽  
Любов Іванівна Калакайло ◽  
Вадим Дмитрович Іщенко ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Animal Feed ◽  
Food Products ◽  
Polymerase Chain Reaction Method ◽  
Dna Identification ◽  
Chain Reaction ◽  
Reaction Method ◽  
Polymerase Chain

Development and Comparison of Four Real-Time Polymerase Chain Reaction Systems for Specific Detection and Quantification ofZea maysL.

2004 ◽  
Vol 52 (15) ◽  
pp. 4632-4637 ◽  
Author(s):  
Marta Hernández ◽  
Marie-Noëlle Duplan ◽  
Georges Berthier ◽  
Marc Vaïtilingom ◽  
Wolfgang Hauser ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Specific Detection ◽  
Chain Reaction ◽  
Reaction Systems ◽  
Polymerase Chain ◽  
Detection And Quantification

scholarly journals Evaluation of a Real Time PCR Assay and a ELISA Method for the Detection of Walnuts and Almonds Allergen Traces in Food Products

2019 ◽  
Vol 8 (3) ◽  
pp. 71 ◽  
Author(s):  
Dimitra Houhoula ◽  
Spiridon Andreas Papatheodorou ◽  
Dimitra Moschou ◽  
Sofia Pappa ◽  
Nikolaos Tsaatazoglou ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Food Industry ◽  
Food Products ◽  
Polymerase Chain Reaction Method ◽  
Raw Material ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Development And Validation ◽  
Improved Accuracy

Food allergens are a well acknowledged issue in food industry and are regulated by legislation. The presence of allergens can either origin from the raw material or due to contamination during production. Allergen information on packaging is mandatory although it cannot be accurate in the case of contamination therefore warnings are used. The purpose of the study is the development and validation of a SYBR Green Real Time Polymerase Chain Reaction method using specific primer pairs based on Jug r 1, Jug r 3, and Jug r 4 allergen-coding sequences to improve the sensitivity of Real Time Polymerase Chain Reaction techniques for detection of walnut and almond traces in commercial food products and its comparison with ELISA methodology in terms of detection ability. A total of 100 samples were collected from local markets and were analyzed by Real Time Polymerase Chain Reaction (RT-PCR) and ELISA methods. The results indicated that 16 samples (16%) were found positive in walnut traces and 18 samples (18%) were found positive in almond traces by Real Time Polymerase Chain Reaction of which Elisa identified 14 samples (14%) positive in walnut traces and 15 samples (15%) positive in almond traces. Among them, 4 samples (25%) that contained walnut traces and 6 samples (33.3%) that contained almond traces had no allergen declaration on their label. The improved accuracy of Real Time Polymerase Chain Reaction underlines the importance of this method for allergen detection and quantification in the food industry

scholarly journals Detection of Cashew Nut DNA in Spiked Baked Goods Using a Real-Time Polymerase Chain Reaction Method

2006 ◽  
Vol 89 (4) ◽  
pp. 1035-1038 ◽  
Author(s):  
Jennifer L Brzezinski
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Food Products ◽  
Polymerase Chain Reaction Method ◽  
Taqman Probe ◽  
Chain Reaction ◽  
2S Albumin ◽  
Baked Goods ◽  
Cashew Nut ◽  
Polymerase Chain

Abstract The detection of potentially allergenic foods, such as tree nuts, in food products is a major concern for the food processing industry. A real-time polymerase chain reaction (PCR) method was designed to determine the presence of cashew DNA in food products. The PCR amplifies a 67 bp fragment of the cashew 2S albumin gene, which is detected with a cashew-specific, dual-labeled TaqMan probe. This reaction will not amplify DNA derived from other tree nut species, such as almond, Brazil nut, hazelnut, and walnut, as well as 4 varieties of peanut. This assay was sensitive enough to detect 5 pg purified cashew DNA as well as cashew DNA in a spiked chocolate cookie sample containing 0.01% (100 mg/kg) cashew.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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