Overexpression of Glutathione S-Transferase Genes in Field λ-Cyhalothrin-Resistant Population of Cydia pomonella: Reference Gene Selection and Expression Analysis

2020 ◽  
Vol 68 (21) ◽  
pp. 5825-5834
Author(s):  
Zi-Han Wei ◽  
Miao Liu ◽  
Chao Hu ◽  
Xue-Qing Yang
Keyword(s):  
Reference Gene ◽  
Expression Analysis ◽  
Gene Selection ◽  
Cydia Pomonella ◽  
Glutathione S Transferase ◽  
Reference Gene Selection ◽  
Resistant Population

scholarly journals Reference gene selection for quantitative real-time PCR (qRT-PCR) expression analysis in Galium aparine L.

PLoS ONE ◽  
2020 ◽  
Vol 15 (2) ◽  
pp. e0226668 ◽  
Author(s):  
Xu Su ◽  
Liuyang Lu ◽  
Yashe Li ◽  
Congai Zhen ◽  
Guilei Hu ◽  
...  
Keyword(s):  
Real Time ◽  
Reference Gene ◽  
Expression Analysis ◽  
Real Time Pcr ◽  
Gene Selection ◽  
Quantitative Real Time Pcr ◽  
Reference Gene Selection ◽  
Qrt Pcr ◽  
Galium Aparine ◽  
Selection For

Reference gene selection and validation for mRNA expression analysis by RT-qPCR in murine M1- and M2-polarized macrophage

2020 ◽  
Vol 47 (4) ◽  
pp. 2735-2748
Author(s):  
Wen Ju ◽  
Tiantian Sun ◽  
Wenyi Lu ◽  
Alhaji Osman Smith ◽  
Yurong Bao ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Reference Gene ◽  
Expression Analysis ◽  
Gene Selection ◽  
Reference Gene Selection ◽  
Mrna Expression Analysis

scholarly journals Reference Gene Selection for Gene Expression Analysis of Oocytes Collected from Dairy Cattle and Buffaloes during Winter and Summer

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e93287 ◽  
Author(s):  
Carolina Habermann Macabelli ◽  
Roberta Machado Ferreira ◽  
Lindsay Unno Gimenes ◽  
Nelcio Antonio Tonizza de Carvalho ◽  
Júlia Gleyci Soares ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dairy Cattle ◽  
Reference Gene ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Gene Selection ◽  
Reference Gene Selection ◽  
Selection For

scholarly journals Reference Gene Selection for Normalizing Gene Expression in Ips Sexdentatus (Coleoptera: Curculionidae: Scolytinae) Under Different Experimental Conditions

2021 ◽  
Vol 12 ◽  
Author(s):  
Gothandapani Sellamuthu ◽  
Shan Amin ◽  
Jan Bílý ◽  
Jirí Synek ◽  
Roman Modlinger ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reference Gene ◽  
Expression Analysis ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Gene Selection ◽  
Experimental Conditions ◽  
Tissue Specific ◽  
Reference Gene Selection ◽  
Ips Sexdentatus

Ips sexdentatus (Coleoptera: Curculionidae: Scolytinae) is one of the most destructive and economically important forest pests. A better understanding of molecular mechanisms underlying its adaptation to toxic host compounds may unleash the potential for future management of this pest. Gene expression studies could be considered as one of the key experimental approaches for such purposes. A suitable reference gene selection is fundamental for quantitative gene expression analysis and functional genomics studies in I. sexdentatus. Twelve commonly used reference genes in Coleopterans were screened under different experimental conditions to obtain accurate and reliable normalization of gene expression data. The majority of the 12 reference genes showed a relatively stable expression pattern among developmental stages, tissue-specific, and sex-specific stages; however, some variabilities were observed during varied temperature incubation. Under developmental conditions, the Tubulin beta-1 chain (β-Tubulin) was the most stable reference gene, followed by translation elongation factor (eEF2) and ribosomal protein S3 (RPS3). In sex-specific conditions, RPS3, β-Tubulin, and eEF2 were the most stable reference genes. In contrast, different sets of genes were shown higher stability in terms of expression under tissue-specific conditions, i.e., RPS3 and eEF2 in head tissue, V-ATPase-A and eEF2 in the fat body, V-ATPase-A and eEF2 in the gut. Under varied temperatures, β-Tubulin and V-ATPase-A were most stable, whereas ubiquitin (UbiQ) and V-ATPase-A displayed the highest expression stability after Juvenile Hormone III treatment. The findings were validated further using real-time quantitative reverse transcription PCR (RT-qPCR)-based target gene expression analysis. Nevertheless, the present study delivers a catalog of reference genes under varied experimental conditions for the coleopteran forest pest I. sexdentatus and paves the way for future gene expression and functional genomic studies on this species.

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