Mechanism Analysis of a Novel Angiotensin-I-Converting Enzyme Inhibitory Peptide from Isochrysis zhanjiangensis Microalgae for Suppressing Vascular Injury in Human Umbilical Vein Endothelial Cells

2020 ◽  
Vol 68 (15) ◽  
pp. 4411-4423
Author(s):  
Jiali Chen ◽  
Li Tan ◽  
Chengyong Li ◽  
Chunxia Zhou ◽  
Pengzhi Hong ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Vascular Injury ◽  
Umbilical Vein ◽  
Mechanism Analysis ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Inhibitory Peptide ◽  
Human Umbilical Vein ◽  
Angiotensin I Converting Enzyme ◽  
Umbilical Vein Endothelial Cells

scholarly journals Down-Regulation of MicroRNA-137 Improves High Glucose-Induced Oxidative Stress Injury in Human Umbilical Vein Endothelial Cells by Up-Regulation of AMPKα1

2016 ◽  
Vol 39 (3) ◽  
pp. 847-859 ◽  
Author(s):  
Jie Li ◽  
Junfeng Li ◽  
Tingting Wei ◽  
Junhua Li
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Protective Effect ◽  
Cell Viability ◽  
Vascular Injury ◽  
High Glucose ◽  
Umbilical Vein ◽  
Down Regulation ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

Background/Aims: To investigate the effects of miR-137 on high glucose (HG)-induced vascular injury, and to establish the mechanism underlying these effects. Methods: Human umbilical vein endothelial cells (HUVECs) were transfected with miR-137 inhibitor or mimic, and then treated with normal or high glucose. Cell viability and apoptosis were detected by using the Cell Counting Kit-8 (CCK-8) assay and flow cytometry, respectively. Reactive oxygen species (ROS), malondialdehyde (MDA), and superoxide dismutase (SOD) were detected by fluorescent probe (DCFH-DA), thiobarbituric acid reaction, and the nitroblue tetrazolium assay, respectively. The mRNA and protein expressions of AMPKα1 were determined by qRT-PCR and Western blotting. Results: Down-regulation of miR-137 dramatically reverted HG-induced decreases in cell viability and SOD levels and increases in apoptosis, ROS and MDA levels. Moreover, bioinformatics analysis predicted that the AMPKα1 was a potential target gene of miR-137. Luciferase reporter assay demonstrated that miR-137 could directly target AMPKα1. AMPKα1 overexpression had the similar effect as miR-137 inhibition. Down-regulation of AMPKα1 in HUVECs transfected with miR-137 inhibitor partially reversed the protective effect of miR-137 inhibition on HG-induced oxidative stress in HUVECs. Conclusion: Down-regulation of miR-137 ameliorates HG-induced injury in HUVECs by overexpression of AMPKα1, leading to increasing cellular reductive reactions and decreasing oxidative stress. These results provide further evidence for protective effect of miR-137 inhibition on HG-induced vascular injury.

scholarly journals A ligand-based approach to investigate the expression and function of angiotensin converting enzyme in intact human umbilical vein endothelial cells

Peptides ◽  
2010 ◽  
Vol 31 (8) ◽  
pp. 1546-1554 ◽  
Author(s):  
Gérémy Abdull Koumbadinga ◽  
Marie-Thérèse Bawolak ◽  
Emilie Marceau ◽  
Albert Adam ◽  
Lajos Gera ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Angiotensin Converting Enzyme ◽  
Umbilical Vein ◽  
Converting Enzyme ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells ◽  
And Function

l-158,809 and (d-Ala7)-angiotensin I/II (1–7) decrease PAI-1 release from human umbilical vein endothelial cells

2002 ◽  
Vol 105 (6) ◽  
pp. 531-536 ◽  
Author(s):  
Masaya Yoshida ◽  
Yasuhisa Naito ◽  
Tetsumei Urano ◽  
Akikazu Takada ◽  
Yumiko Takada
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Angiotensin I ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells ◽  
Pai 1
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