Wavelength-Dependent Damage to Adenoviral Proteins Across the Germicidal UV Spectrum

2017 ◽  
Vol 52 (1) ◽  
pp. 223-229 ◽  
Author(s):  
Sara E. Beck ◽  
Natalie M. Hull ◽  
Christopher Poepping ◽  
Karl G. Linden
Keyword(s):  
Uv Spectrum ◽  
Adenoviral Proteins

Ultrastructural changes in murine lymphoma cells exposed to ultraviolet-A radiation

1991 ◽  
Vol 49 ◽  
pp. 104-105
Author(s):  
Delma P. Thomas ◽  
Dianne E. Godar
Keyword(s):  
Medical Devices ◽  
Blood Cells ◽  
White Blood Cells ◽  
Consumer Products ◽  
Ultrastructural Changes ◽  
Ultraviolet A ◽  
Uv Spectrum ◽  
Lymphoma Cells ◽  
Murine Lymphoma ◽  
Transmission Electron

Ultraviolet radiation (UVR) from all three waveband regions of the UV spectrum, UVA (320-400 nm), UVB (290-320 nm), and UVC (200-290 nm), can be emitted by some medical devices and consumer products. Sunlamps can expose the blood to a considerable amount of UVR, particularly UVA and/or UVB. The percent transmission of each waveband through the epidermis to the dermis, which contains blood, increases in the order of increasing wavelength: UVC (10%) < UVB (20%) < UVA (30%). To investigate the effects of UVR on white blood cells, we chose transmission electron microscopy to examine the ultrastructure changes in L5178Y-R murine lymphoma cells.

DYNAMICS OF BARLEY FLAVONOID AND PRODUCTIVITY UNDER UF-A RADIATION

1970 ◽  
pp. 14-18
Author(s):  
L. I. Goncharova ◽  
P. N. Tsygvintsev ◽  
О. А. Guseva
Keyword(s):  
Grain Yield ◽  
Defense Mechanisms ◽  
Antioxidant Defense ◽  
Malonic Dialdehyde ◽  
Growth Period ◽  
Negative Effects ◽  
Uv Spectrum ◽  
Quantum Energy ◽  
Dry Soil ◽  
Universal Mechanism

The effect of increased UV-A radiation during the ontogeny of barley plants of the Vladimir variety in the vegetation experiment was studied. Changes in the content of malonic dialdehyde, flavonoids and grain yield were revealed. UV-A radiation as compared to UV-B radiation, has lower quantum energy and can have both positive and negative effects on plant regulatory and photosynthetic processes. One of the most damaging effects of increased levels of UV-A radiation is oxidative stress, which causes lipid peroxidation of biological membranes. The existence of a plant cell in such conditions is possible only thanks to a system of antioxidant defense mechanisms. The accumulation of phenolic compounds under the action of UV radiation is a universal mechanism of protection against photodamage, which was formed in the early stages of the evolution of photoautotrophic organisms. Flavonoids are localized in the epidermis of plant tissues and act as an internal filter. The content of flavonoids is determined by the genotype and due to ontogenetic patterns. Plants were grown in a greenhouse, in vessels containing 4.5 kg of air-dry soil. The repetition is threefold (3 vessels in each variant). Sowing density - 13 plants in each vessel. As a source of UV-A radiation used lamps Black Light BLUE company Philips. Plants were irradiated for 5 hours a day from 10 to 15 hours at 13, 25, 34, 43 and 52 stages of organogenesis. The magnitude of the daily biologically effective dose of UV-A radiation was 60.7 kJ / m2. The solar part of the UV spectrum in the vegetation experiment was absent in the greenhouse. The nature of changes in the content of flavonoids under the action of UV-A irradiation during the growing season of plants with the dynamics of the oxidative process has been established. The first maximum was observed during the vegetative growth period, the second - at the earing stage. The data obtained indicate that flavonoids have ontogenetic conditionality and perform photoprotective functions. The increase in their content under the action of UV-A radiation is accompanied by an increase in resistance to photodamage, which is confirmed by the formation of grain yield.

scholarly journals Thrombotic Thrombocytopenia after COVID-19 Vaccination: In Search of the Underlying Mechanism

Vaccines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 559
Author(s):  
Piotr Rzymski ◽  
Bartłomiej Perek ◽  
Robert Flisiak
Keyword(s):  
Direct Interaction ◽  
Underlying Mechanism ◽  
The United States ◽  
Cross Reactivity ◽  
Spike Protein ◽  
Vaccine Hesitancy ◽  
Future Research ◽  
Platelet Factor ◽  
Precautionary Measures ◽  
Adenoviral Proteins

The rollout of COVID-19 vaccines brings hope for successful pandemic mitigation and getting the transmission of SARS-CoV-2 under control. The vaccines authorized in Europe displayed a good safety profile in the clinical trials. However, during their post-authorization use, unusual thrombotic events associated with thrombocytopenia have rarely been reported for vector vaccines. This led to the temporary suspension of the AZD1222 vaccine (Oxford/AstraZeneca) in various European countries and the Ad26.COV2 vaccine (Janssen/Johnson&Johnson) in the United States, with regulatory bodies launching investigations into potential causal associations. The thromboembolic reactions were also rarely reported after mRNA vaccines. The exact cause of these adverse effects remains to be elucidated. The present paper outlines the hypotheses on the mechanisms behind the very rare thrombotic thrombocytopenia reported after the COVID-19 vaccination, along with currently existing evidence and future research prospects. The following are discussed: (i) the role of antibodies against platelet factor 4 (PF4), (ii) the direct interaction between adenoviral vector and platelets, (iii) the cross-reactivity of antibodies against SARS-CoV-2 spike protein with PF4, (iv) cross-reactivity of anti-adenovirus antibodies and PF4, (v) interaction between spike protein and platelets, (vi) the platelet expression of spike protein and subsequent immune response, and (vii) the platelet expression of other adenoviral proteins and subsequent reactions. It is also plausible that thrombotic thrombocytopenia after the COVID-19 vaccine is multifactorial. The elucidation of the causes of these adverse events is pivotal in taking precautionary measures and managing vaccine hesitancy. It needs to be stressed, however, that the reported cases are currently sporadic and that the benefits of COVID-19 vaccines vastly outweigh their potential risks.

scholarly journals Enhanced ultraviolet absorption in graphene by aluminum and magnesium hole-arrays

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xueling Cheng ◽  
Yunshan Wang
Keyword(s):  
Numerical Study ◽  
Uv Spectroscopy ◽  
Single Layer ◽  
Hybrid Structure ◽  
Uv Absorption ◽  
Single Layer Graphene ◽  
Visible Range ◽  
Uv Spectrum ◽  
Hole Arrays ◽  
Uv Range

AbstractOptoelectronic devices in the UV range have many applications including deep-UV communications, UV photodetectors, UV spectroscopy, etc. Graphene has unique exciton resonances, that have demonstrated large photosensitivity across the UV spectrum. Enhancing UV absorption in graphene has the potential to boost the performance of the various opto-electronic devices. Here we report numerical study of UV absorption in graphene on aluminum and magnesium hole-arrays. The absorption in a single-layer graphene on aluminum and magnesium hole-arrays reached a maximum value of 28% and 30% respectively, and the absorption peak is tunable from the UV to the visible range. The proposed graphene hybrid structure does not require graphene to be sandwiched between different material layers and thus is easy to fabricate and allows graphene to interact with its surroundings.

Rare Earth and Tetra Hydroxymethyl Phosphonium Sulfate Combination Tanning

2012 ◽  
Vol 479-481 ◽  
pp. 430-433
Author(s):  
Ya Li ◽  
Shuang Xi Shao ◽  
Lan Jiang ◽  
Kai Qi Shi
Keyword(s):  
Rare Earth ◽  
Tartaric Acid ◽  
Alkaline Ph ◽  
Uv Spectrum ◽  
Shrinkage Temperature

A new chrome free tanning system of rare earth (RE)-THPS has been developed in this study. It has been proved by UV spectrum that THPS and RE (CeCl3) can coordinate under certain conditions. The influence of CeCl3and THPS’s ratio, pH and ligands on the shrinkage temperature (Ts) was studied. Higher Ts was obtained while the tannage was processed in the sequence of THPS followed by CeCl3. The alkaline pH should be controlled between 5.5 and 6.0 to give ideal tanning effect. It was also found that employing of 0.5% tartaric acid in this combination tanning system can give high Ts of 89°C.

scholarly journals IUE Studies of Luminous X-Ray Binaries

1983 ◽  
Vol 6 ◽  
pp. 648-648
Author(s):  
J.B. Hutchings
Keyword(s):  
Magellanic Clouds ◽  
Thick Shell ◽  
High Luminosity ◽  
Uv Spectrum ◽  
X Ray ◽  
Unique Object ◽  
The Future ◽  
Orbit Inclination ◽  
Supercritical Accretion ◽  
X Ray Binaries

IUE has been used to study 11 high luminosity X-ray binaries, of which 3 are in the Magellanic Clouds. In the supergiant systems, X-ray ionisation bubbles have been found in most cases, leading to a greater understanding of the winds and accretion processes. Further studies of precessing objects such as LMC X-4 with IUE and ST are clearly of considerable interest, relating to X-ray heating and blanketing. Detailed studies of the Cyg X-l ionisation bubble may resolve the long standing puzzle of its orbit inclination and masses. UV continua have furnished valuable information on extinction, temperatures and luminosities, and the presence of non-stellar (i.e. disk) luminosity. Here too, more detailed studies are clearly indicated for the future. A unique object of interest is the LMC transient 0538-66 whose UV spectrum has quasarlike lines and luminosity which varies oppositely to the visible. This may be a case of supercritical accretion generating an optically thick shell (“disk”) about the pulsar.

scholarly journals Fast Dissolving Electrospun Nanofibers Fabricated from Jelly Fig Polysaccharide/Pullulan for Drug Delivery Applications

Polymers ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 241
Author(s):  
Thangavel Ponrasu ◽  
Bei-Hsin Chen ◽  
Tzung-Han Chou ◽  
Jia-Jiuan Wu ◽  
Yu-Shen Cheng
Keyword(s):  
Drug Delivery ◽  
Water Vapor Permeability ◽  
Electrospun Nanofibers ◽  
Average Diameter ◽  
Xrd Analysis ◽  
Water Soluble ◽  
Vapor Permeability ◽  
Uv Spectrum ◽  
Sem Images ◽  
Triton X

The fast-dissolving drug delivery systems (FDDDSs) are developed as nanofibers using food-grade water-soluble hydrophilic biopolymers that can disintegrate fast in the oral cavity and deliver drugs. Jelly fig polysaccharide (JFP) and pullulan were blended to prepare fast-dissolving nanofiber by electrospinning. The continuous and uniform nanofibers were produced from the solution of 1% (w/w) JFP, 12% (w/w) pullulan, and 1 wt% Triton X-305. The SEM images confirmed that the prepared nanofibers exhibited uniform morphology with an average diameter of 144 ± 19 nm. The inclusion of JFP in pullulan was confirmed by TGA and FTIR studies. XRD analysis revealed that the increased crystallinity of JFP/pullulan nanofiber was observed due to the formation of intermolecular hydrogen bonds. The tensile strength and water vapor permeability of the JFP/pullulan nanofiber membrane were also enhanced considerably compared to pullulan nanofiber. The JFP/pullulan nanofibers loaded with hydrophobic model drugs like ampicillin and dexamethasone were rapidly dissolved in water within 60 s and release the encapsulants dispersive into the surrounding. The antibacterial activity, fast disintegration properties of the JFP/pullulan nanofiber were also confirmed by the zone of inhibition and UV spectrum studies. Hence, JFP/pullulan nanofibers could be a promising carrier to encapsulate hydrophobic drugs for fast-dissolving/disintegrating delivery applications.

UV-microscopic analysis of acetylated spruce and birch cell walls

Holzforschung ◽  
2004 ◽  
Vol 58 (5) ◽  
pp. 483-488 ◽  
Author(s):  
Christian Hansmann ◽  
Manfred Schwanninger ◽  
Barbara Stefke ◽  
Barbara Hinterstoisser ◽  
Wolfgang Gindl
Keyword(s):  
Cell Walls ◽  
Middle Lamella ◽  
Microscopic Analysis ◽  
Weight Percent ◽  
Cellular Level ◽  
Uv Spectrum ◽  
Spectral Shifts ◽  
Microscopy Analysis ◽  
Absorbance Peak ◽  
Absorbance Spectra

Abstract Spruce and birch earlywood was acetylated to different weight percent gains using three different acetylation procedures. The absorbance spectra of secondary cell wall and compound cell corner middle lamella were determined by means of UV microscopy. Analysis of the spectra showed that the characteristic lignin absorbance peak in the UV spectrum of wood around 280 nm shifted to shorter wavelengths in acetylated samples. A distinct relationship between achieved weight percent gains after acetylation and observed spectral shifts could be established revealing a certain potential to measure acetylation on a cellular level by means of UV microscopy.

scholarly journals High‐Ionization Forbidden Lines in the UV Spectrum of AG Draconis

2006 ◽  
Vol 650 (2) ◽  
pp. 1091-1095 ◽  
Author(s):  
P. R. Young ◽  
A. K. Dupree ◽  
B. R. Espey ◽  
S. J. Kenyon
Keyword(s):  
Uv Spectrum ◽  
High Ionization

scholarly journals Development and Validation of UV Spectrophotometric Method for the Determination of Cefuroxime Axetil in Bulk and Pharmaceutical Formulation

2013 ◽  
Vol 6 (1) ◽  
pp. 133-141 ◽  
Author(s):  
S. Binte Amir ◽  
M. A. Hossain ◽  
M. A. Mazid
Keyword(s):  
Spectrophotometric Method ◽  
Cost Effective ◽  
Cefuroxime Axetil ◽  
Pharmaceutical Formulation ◽  
Uv Spectrum ◽  
Accuracy And Precision ◽  
Relative Standard ◽  
Label Claim ◽  
Development And Validation

The present study was undertaken to develop and validate a simple, sensitive, accurate, precise and reproducible UV spectrophotometric method for cefuroxime axetil using methanol as solvent. In this method the simple UV spectrum of cefuroxime axetil in methanol was obtained which exhibits absorption maxima (?max) at 278 nm. The quantitative determination of the drug was carried out at 278 nm and Beer’s law was obeyed in the range of (0.80-3.60) µg/ml. The proposed method was applied to pharmaceutical formulation and percent amount of drug estimated (95.6% and 96%) was found in good agreement with the label claim. The developed method was successfully validated with respect to linearity, specificity, accuracy and precision. The method was shown linear in the mentioned concentrations having line equation y = 0.05x + 0.048 with correlation coefficient of 0.995. The recovery values for cefuroxime axetil ranged from 99.85-100.05. The relative standard deviation of six replicates of assay was less than 2%. The percent relative standard deviations of inter-day precision ranged between 1.45-1.92% and intra-day precision of cefuroxime axetil was 0.96-1.51%. Hence, proposed method was precise, accurate and cost effective.  Keywords: UV-Vis spectrophotometer; Method validation; Cefuroxime axetil; Recovery studies.  © 2013 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.   doi: http://dx.doi.org/10.3329/jsr.v6i1.14879 J. Sci. Res. 6 (1), 133-141 (2013)  

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