Reduction of Human Norovirus GI, GII, and Surrogates by Peracetic Acid and Monochloramine in Municipal Secondary Wastewater Effluent

2017 ◽  
Vol 51 (20) ◽  
pp. 11918-11927 ◽  
Author(s):  
Nathan Dunkin ◽  
ShihChi Weng ◽  
Caroline G. Coulter ◽  
Joseph G. Jacangelo ◽  
Kellogg J. Schwab
Keyword(s):  
Peracetic Acid ◽  
Wastewater Effluent ◽  
Human Norovirus

Inactivation of Murine Norovirus and MS2 Bacteriophage by Peracetic Acid and Monochloramine in Municipal Secondary Wastewater Effluent

2017 ◽  
Vol 2017 (9) ◽  
pp. 3095-3102
Author(s):  
Joseph G Jacangelo ◽  
Nathan Dunkin ◽  
Shih-Chi Weng ◽  
James McQuarrie ◽  
Kati Bell ◽  
...  
Keyword(s):  
Peracetic Acid ◽  
Wastewater Effluent ◽  
Murine Norovirus ◽  
Ms2 Bacteriophage

Comparative Inactivation of Murine Norovirus and MS2 Bacteriophage by Peracetic Acid and Monochloramine in Municipal Secondary Wastewater Effluent

2017 ◽  
Vol 51 (5) ◽  
pp. 2972-2981 ◽  
Author(s):  
Nathan Dunkin ◽  
ShihChi Weng ◽  
Kellogg J. Schwab ◽  
James McQuarrie ◽  
Kati Bell ◽  
...  
Keyword(s):  
Peracetic Acid ◽  
Wastewater Effluent ◽  
Murine Norovirus ◽  
Ms2 Bacteriophage

Effects of pH Variability on Peracetic Acid Reduction of Human Norovirus GI, GII RNA, and Infectivity Plus RNA Reduction of Selected Surrogates

2018 ◽  
Vol 11 (1) ◽  
pp. 76-89 ◽  
Author(s):  
Nathan Dunkin ◽  
Caroline Coulter ◽  
ShihChi Weng ◽  
Joseph G. Jacangelo ◽  
Kellogg J. Schwab
Keyword(s):  
Peracetic Acid ◽  
Human Norovirus ◽  
Effects Of Ph ◽  
Acid Reduction

scholarly journals Inactivation of human rotavirus, SA11 and other enteric viruses in effluent by disinfectants

1984 ◽  
Vol 93 (1) ◽  
pp. 157-163 ◽  
Author(s):  
M. Harakeh ◽  
M. Butler
Keyword(s):  
Chlorine Dioxide ◽  
Peracetic Acid ◽  
Viral Antigen ◽  
Enteric Viruses ◽  
Wastewater Effluent ◽  
Human Rotavirus

SUMMARYA preparation of infectious human rotavirus, isolated from faeces and resuspended in wastewater effluent, was shown to be inactivated by chlorine, chlorine dioxide, ozone and peracetic acid. Infectivity was assayed in MA 104 cells by the detection of cell-associated viral antigen by immunofluorescence. The inactivation curves were similar to those reported for other enteric viruses. Human rotavirus was at least as resistant as poliovirus, coxsackievirus, echovirus and f2coliphage and was strikingly less sensitive to inactivation than the simian rotavirus, SA11. The latter was generally the most sensitive of the six tested viruses yet is often taken as being representative of the human rotaviruses.

scholarly journals The Basis of Peracetic Acid Inactivation Mechanisms for Rotavirus and Tulane Virus under Conditions Relevant for Vegetable Sanitation

2020 ◽  
Vol 86 (19) ◽  
Author(s):  
Miyu Fuzawa ◽  
Hezi Bai ◽  
Joanna L. Shisler ◽  
Thanh H. Nguyen
Keyword(s):  
Peracetic Acid ◽  
Molecular Mechanisms ◽  
Magnetic Beads ◽  
Virus Titer ◽  
Human Norovirus ◽  
Porcine Gastric Mucin ◽  
Genome Damage ◽  
Versus Protein ◽  
Receptor Interactions ◽  
Tulane Virus

ABSTRACT We determined the disinfection efficacy and inactivation mechanisms of peracetic acid (PAA)-based sanitizer using pH values relevant for vegetable sanitation against rotavirus (RV) and Tulane virus (TV; a human norovirus surrogate). TV was significantly more resistant to PAA disinfection than RV: for a 2-log10 reduction of virus titer, RV required 1 mg/liter PAA for 3.5 min of exposure, while TV required 10 mg/liter PAA for 30 min. The higher resistance of TV can be explained, in part, by significantly more aggregation of TV in PAA solutions. The PAA mechanisms of virus inactivation were explored by quantifying (i) viral genome integrity and replication using reverse transcription-quantitative PCR (RT-qPCR) and (ii) virus-host receptor interactions using a cell-free binding assay with porcine gastric mucin conjugated with magnetic beads (PGM-MBs). We observed that PAA induced damage to both RV and TV genomes and also decreased virus-receptor interactions, with the latter suggesting that PAA damages viral proteins important for binding its host cell receptors. Importantly, the levels of genome-versus-protein damage induced by PAA were different for each virus. PAA inactivation correlated with higher levels of RV genome damage than of RV-receptor interactions. For PAA-treated TV, the opposite trends were observed. Thus, PAA inactivates each of these viruses via different molecular mechanisms. The findings presented here potentially contribute to the design of a robust sanitation strategy for RV and TV using PAA to prevent foodborne disease. IMPORTANCE In this study, we examined the inactivation mechanisms of peracetic acid (PAA), a sanitizer commonly used for postharvest vegetable washing, for two enteric viruses: Tulane virus (TV) as a human norovirus surrogate and rotavirus (RV). PAA disinfection mechanisms for RV were mainly due to genome damage. In contrast, PAA disinfection in TV was due to damage of the proteins important for binding to its host receptor. We also observed that PAA triggered aggregation of TV to a much greater extent than RV. These studies demonstrate that different viruses are inactivated via different PAA mechanisms. This information is important for designing an optimal sanitation practice for postharvest vegetable washing to minimize foodborne viral diseases.

scholarly journals Impacts of virus processing on human norovirus GI and GII persistence during disinfection of municipal secondary wastewater effluent

2018 ◽  
Vol 134 ◽  
pp. 1-12 ◽  
Author(s):  
Nathan Dunkin ◽  
ShihChi Weng ◽  
Caroline G. Coulter ◽  
Joseph G. Jacangelo ◽  
Kellogg J. Schwab
Keyword(s):  
Wastewater Effluent ◽  
Human Norovirus

scholarly journals Effectiveness of Consumers Washing with Sanitizers to Reduce Human Norovirus on Mixed Salad

Foods ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 637 ◽  
Author(s):  
Anfruns-Estrada ◽  
Bottaro ◽  
Pintó ◽  
Guix ◽  
Bosch
Keyword(s):  
Sodium Hypochlorite ◽  
Chlorine Dioxide ◽  
Peracetic Acid ◽  
Acute Gastroenteritis ◽  
Food Industry ◽  
Risk Of Infection ◽  
Propidium Monoazide ◽  
Human Norovirus ◽  
Microbiological Safety ◽  
Fresh Products

Human norovirus (HuNoV) is a foremost cause of domestically acquired foodborne acute gastroenteritis and outbreaks. Despite industrial efforts to control HuNoV contamination of foods, its prevalence in foodstuffs at retail is significant. HuNoV infections are often associated with the consumption of contaminated produce, including ready-to-eat (RTE) salads. Decontamination of produce by washing with disinfectants is a consumer habit which could significantly contribute to mitigate the risk of infection. The aim of our study was to measure the effectiveness of chemical sanitizers in inactivating genogroup I and II HuNoV strains on mixed salads using a propidium monoazide (PMAxx)-viability RTqPCR assay. Addition of sodium hypochlorite, peracetic acid, or chlorine dioxide significantly enhanced viral removal as compared with water alone. Peracetic acid provided the highest effectiveness, with log10 reductions on virus levels of 3.66 ± 0.40 and 3.33 ± 0.19 for genogroup I and II, respectively. Chlorine dioxide showed lower disinfection efficiency. Our results provide information useful to the food industry and final consumers for improving the microbiological safety of fresh products in relation to foodborne viruses.

Обеззараживание семян капусты от сосудистого бактериоза

2018 ◽  
Author(s):  
A.T. Orynbayev ◽  
F.S. Dzhalilov
Keyword(s):  
Peracetic Acid ◽  
Biological Efficiency ◽  
Black Rot ◽  
Presowing Treatment

Приведены данные двухлетнего исследования по поиску эффективных препаратов для обеззараживания семян капусты от сосудистого бактериоза. Наилучший результат был получен при предпосевной обработке 0,15%-ной надуксусной кислотой. При этом биологическая эффективность составляла 94,1–97,6%, что значительно превышало соответствующий показатель в эталонном варианте – Фитолавин, ВРК, 0,2% (69,0–72,0%). Показано, что заблаговременная обработка семян НУК (за 550 суток до посева) не оказывала достоверного влияния на их лабораторную всхожесть.Presented data are the result of a two-year study aimed at identifying effective preparations for the disinfection of cabbage seeds from black rot. The best result was obtained by presowing treatment with 0.15% peracetic acid. In this case, the biological efficiency was 94.1–97.6%, which significantly exceeded the corresponding indicator in the reference variant – Phytolavin, VRK, 0.2% (69.0–72.0%). The study has not revealed any significant effect of advance treatment of seeds with peracetic acid (550 days before sowing) on their laboratory germination.

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