Evaluating the Toxicity of Silver Nanoparticles by Detecting Phosphorylation of Histone H3 in Combination with Flow Cytometry Side-Scattered Light

2015 ◽  
Vol 49 (8) ◽  
pp. 5003-5012 ◽  
Author(s):  
Xiaoxu Zhao ◽  
Yuko Ibuki
Keyword(s):  
Flow Cytometry ◽  
Silver Nanoparticles ◽  
Scattered Light ◽  
Histone H3

Detection of intracellular silver nanoparticles using flow cytometry

2018 ◽  
pp. 223-226
Author(s):  
С.И. Каба ◽  
А.А. Соколовская
Keyword(s):  
Flow Cytometry ◽  
Silver Nanoparticles ◽  
Endothelial Cells ◽  
Silver Nanoparticle ◽  
Cell Uptake ◽  
Control Samples

Продемонстрировано обнаружение наночастиц серебра во внутриклеточном пространстве с помощью проточной цитофлуориметрии. В эндотелиальных клетках линии EA.hy926, инкубированных в растворе, содержащем 2 мкг/мл наносеребра, измеряли боковое светорассеяние. По сравнению с контрольными образцами этот параметр возрастал, в то время как прочие значимые характеристики не изменялись. Это подтверждает чувствительность метода к изменившемуся состоянию клеток и указывает на поглощение наночастиц серебра клетками при концентрации ниже токсической. The study demonstrated a possibility for detection of intracellular silver nanoparticles using flow cytometry. The parameter used in this work, side scattering, was measured in EA.hy926 endothelial cells incubated in a 2 mg/ml silver nanoparticle solution. This parameter was increased compared to control samples. Therefore, this technique was sensitive to changes in the cell status and suggested the cell uptake of the particles under the subtoxic conditions.

Preliminary Study on In Situ Realtime Quantitation of Target Bacteria on the Principle of Flow Cytometry

2017 ◽  
Vol 262 ◽  
pp. 224-227
Author(s):  
Gen Murakami ◽  
Yuichi Sugai ◽  
Kyuro Sasaki
Keyword(s):  
Flow Cytometry ◽  
Bacterial Culture ◽  
Scattered Light ◽  
Bacterial Suspension ◽  
Culture Solution ◽  
Bacterial Cells ◽  
Monitoring Wells ◽  
Measurement Principle ◽  
Preliminary Study

In-situ realtime method that can monitor the target bacteria should be used to determine the real situation of the bacteria in deep parts of heaps in heap bioleaching plants. This study suggest to apply flow cytometry technology to in-situ realtime monitoring of target bacteria. Flow cytometry is a method that can rapidly quantify the bacterial cells in bacterial suspension based on the detection of lights that are emitted from bacterial cells. In this study, we estimated the possibility of the application of flow cytometry to the selective detection of target bacteria. The bacterial culture solution that had been diluted by water including other bacteria was provided for fluorescence spectral analysis and scattered light analysis that were functions of flow cytometry. Our target bacteria could be selectively detected by those analyses in this study, therefore, it was shown that the flow cytometry could be useful for detecting target bacteria selectively. Because the measurement principle of flow cytometry is quite simple, it can be expected to be installed into deep heaps through the monitoring wells and determine the dominance of target bacteria in-situ and realtime in the future.

The Future of Flow Cytometry in Biotechnology: The Response to Diversity and Complexity

2005 ◽  
Author(s):  
Larry A. Sklar
Keyword(s):  
Flow Cytometry ◽  
Scattered Light ◽  
National Laboratory ◽  
Modern World ◽  
Light Sources ◽  
Main Function ◽  
Hydrodynamic Focusing ◽  
Los Alamos National Laboratory ◽  
The Future ◽  
Dependent Cell

Flow cytometry is a mature technology: Instruments recognizable as having elements of modern flow cytometers date back at least 30 years. There are many good sources for information about the essential features of flow cytometers, how they operate, and how they have been used. For the purposes of this book, it is necessary to know that flow cytometers have fluidic, optical, electronic, computational, and mechanical features. The main function of the fluidic components is to use hydrodynamic focusing to create a stable particle stream in which particles are aligned in single file within a sheath stream, so that the particles can be analyzed and sorted. The main functions of the optical components are to allow the particles to be illuminated by one or more lasers or other light sources and to allow scattered light as well as multiple fluorescence signals to be resolved and be routed to individual detectors. The electronics coordinate these functions, from the acquisition of the signals (pulse collection, pulse analysis, triggering, time delay, data, gating, detector control) to forming and charging individual droplets, and to making sort decisions. The computational components are directed at postacquisition data display and analysis, analysis of multivariate populations and multiplexing assays, and calibration and analysis of time-dependent cell or reaction phenomena. Mechanical components are now being integrated with flow cytometers to handle plates of samples and to coordinate automation such as the movement of a cloning tray with the collection of the droplets. The reader is directed to a concise description of these processes in Robinson’s article in the Encyclopedia of Biomaterials and Biomedical Engineering. This book was conceived of to provide a perspective on the future of flow cytometry, and particularly its application to biotechnology. It attempts to answer the question I heard repeatedly, especially during my association with the National Institutes of Health–funded National Flow Cytometry Resource at Los Alamos National Laboratory: What is the potential for innovation in flow cytometer design and application? This volume brings together those approaches that identify the unique contributions of flow cytometry to the modern world of biotechnology.

scholarly journals Detection of silver nanoparticles in cells by flow cytometry using light scatter and far-red fluorescence

2013 ◽  
pp. n/a-n/a ◽  
Author(s):  
R. M. Zucker ◽  
K. M. Daniel ◽  
E. J. Massaro ◽  
S. J. Karafas ◽  
L. L. Degn ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Silver Nanoparticles ◽  
Light Scatter ◽  
Red Fluorescence ◽  
In Cells

Flow cytometry evaluation of in vitro cellular necrosis and apoptosis induced by silver nanoparticles

2015 ◽  
Vol 85 ◽  
pp. 45-51 ◽  
Author(s):  
Girish Kumar ◽  
Heba Degheidy ◽  
Brendan J. Casey ◽  
Peter L. Goering
Keyword(s):  
Flow Cytometry ◽  
Silver Nanoparticles ◽  
Cellular Necrosis

Multiparameter Analysis: Application to Vaccine Analysis

2005 ◽  
Author(s):  
Mario Roederer ◽  
Stephen C. De Rosa
Keyword(s):  
Flow Cytometry ◽  
Disease Progression ◽  
Scattered Light ◽  
Surrogate Marker ◽  
Basic Research ◽  
Major Effect ◽  
Fluorescence Measurement ◽  
Multiple Parameters ◽  
Aids Epidemic ◽  
Multiparameter Analysis

Fluorescence-based flow cytometry was introduced in the late 1960s and is now used extensively both in basic research and in the clinic. Flow cytometry allows not only for the rapid multiparametric analysis of cells on a cell-by-cell basis but also for the viable separation, or sorting, of highly purified populations of cells. In this chapter, we will discuss only the analysis aspects. The earliest flow cytometry experiments had three parameters: one fluorescence measurement and two scattered light signals. An early “one-color” experiment successfully separated antibody-secreting B cells from mouse splenocytes. This and other early studies quickly demonstrated the usefulness of this technology in immunological studies. However, measurement of only one fluorescence was a limitation. By adding detectors collecting light in specified wavelength ranges, multiple fluorescence measurements could be made simultaneously. By 1984, four-color fluorescence experiments could be routinely performed, at least in the most sophisticated flow cytometry laboratories, but it took another 10 years before most laboratories could perform routine three-color experiments. One reason for this delay is that it took some time to recognize the need for measuring multiple parameters in addressing questions that explored the complexity of the immune system. Another reason was that it was not until the late 1980s that fourcolor benchtop instrumentation became available. The AIDS epidemic also had a major effect on the expansion of flow cytometry into the research community, as early in the epidemic, the enumeration of CD4 was found to serve as a surrogate marker for disease progression. During this period, we were examining a number of functionally-important T cell subsets in HIV-infected adults and children, including naïve and memory, using three-color flow cytometry. These studies demonstrated clearly for the first time the loss of both CD4 and CD8 naïve T cells during HIV disease progression. This loss had not been previously recognized either because appropriate combinations of reagents were not used or because the studies were limited to two colors. Having demonstrated that multiple markers used in combination could lead to clinically relevant findings that were previously missed, we wondered how many other important subsets could be detected by measuring additional parameters.

scholarly journals Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Daniel Kage ◽  
Kerstin Heinrich ◽  
Konrad v. Volkmann ◽  
Jenny Kirsch ◽  
Kristen Feher ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
High Throughput ◽  
Pulse Shape ◽  
Single Cells ◽  
Scattered Light ◽  
Free Cell ◽  
Hek293 Cells ◽  
Label Free ◽  
Functional Changes

AbstractFlow cytometers are robust and ubiquitous tools of biomedical research, as they enable high-throughput fluorescence-based multi-parametric analysis and sorting of single cells. However, analysis is often constrained by the availability of detection reagents or functional changes of cells caused by fluorescent staining. Here, we introduce MAPS-FC (multi-angle pulse shape flow cytometry), an approach that measures angle- and time-resolved scattered light for high-throughput cell characterization to circumvent the constraints of conventional flow cytometry. In order to derive cell-specific properties from the acquired pulse shapes, we developed a data analysis procedure based on wavelet transform and k-means clustering. We analyzed cell cycle stages of Jurkat and HEK293 cells by MAPS-FC and were able to assign cells to the G1, S, and G2/M phases without the need for fluorescent labeling. The results were validated by DNA staining and by sorting and re-analysis of isolated G1, S, and G2/M populations. Our results demonstrate that MAPS-FC can be used to determine cell properties that are otherwise only accessible by invasive labeling. This approach is technically compatible with conventional flow cytometers and paves the way for label-free cell sorting.

scholarly journals Biophysical comparison of four silver nanoparticles coatings using microscopy, hyperspectral imaging and flow cytometry

PLoS ONE ◽  
2019 ◽  
Vol 14 (7) ◽  
pp. e0219078 ◽  
Author(s):  
Robert M. Zucker ◽  
Jayna Ortenzio ◽  
Laura L. Degn ◽  
Jeremy M. Lerner ◽  
William K. Boyes
Keyword(s):  
Flow Cytometry ◽  
Silver Nanoparticles ◽  
Hyperspectral Imaging

Antimicrobial properties of biosynthesized silver nanoparticles studied by flow cytometry and related techniques

2016 ◽  
Vol 37 (5-6) ◽  
pp. 752-761 ◽  
Author(s):  
Viorica Railean-Plugaru ◽  
Pawel Pomastowski ◽  
Katarzyna Rafinska ◽  
Magdalena Wypij ◽  
Wojciech Kupczyk ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Silver Nanoparticles ◽  
Antimicrobial Properties
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