Food, Beverage, and Feedstock Processing Facility Wastewater: a Unique and Underappreciated Source of Contaminants to U.S. Streams

AbstractEfficient and accurate identification of microorganisms throughout the food chain can potentially allow the identification of sources of contamination and the timely implementation of control measures. High throughput DNA sequencing represents a potential means through which microbial monitoring can be enhanced. While Illumina sequencing platforms are most typically used, newer portable platforms, such as the Oxford Nanopore Technologies (ONT) MinION, offer the potential for rapid analysis of food chain microbiomes. Initial assessment of the ability of rapid MinION-based sequencing to identify microbes within a simple mock metagenomic mixture is performed. Subsequently, we compare the performance of both ONT and Illumina sequencing for environmental monitoring of an active food processing facility. Overall, ONT MinION sequencing provides accurate classification to species level, comparable to Illumina-derived outputs. However, while the MinION-based approach provides a means of easy library preparations and portability, the high concentrations of DNA needed is a limiting factor.

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An Educational Digital Image Processing Facility

Computer ◽

10.1109/c-m.1977.217823 ◽

1977 ◽

Vol 10 (8) ◽

pp. 48-53 ◽

Cited By ~ 2

Author(s):

H.C. Andrews

Keyword(s):

Image Processing ◽

Digital Image Processing ◽

Digital Image ◽

Processing Facility

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Defining Event Reporting In The Cell Processing Facility (CPF)

Biology of Blood and Marrow Transplantation ◽

10.1016/j.bbmt.2009.12.118 ◽

2010 ◽

Vol 16 (2) ◽

pp. S189-S190

Author(s):

M. Merchant ◽

M. Kletzel ◽

T. Shook ◽

M. Villa ◽

R. Meagher ◽

...

Keyword(s):

Event Reporting ◽

Cell Processing ◽

Processing Facility

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Discrete simulation development for a proposed shipyard steel processing facility

Proceeding of the 2001 Winter Simulation Conference (Cat. No.01CH37304) ◽

10.1109/wsc.2001.977388 ◽

2002 ◽

Cited By ~ 6

Author(s):

D.L. Williams ◽

D.A. Finke ◽

D.J. Medeiros ◽

M.T. Traband

Keyword(s):

Discrete Simulation ◽

Processing Facility ◽

Steel Processing

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Dissemination of Cronobacter spp. (Enterobacter sakazakii) in a Powdered Milk Protein Manufacturing Facility

Applied and Environmental Microbiology ◽

10.1128/aem.00745-08 ◽

2008 ◽

Vol 74 (19) ◽

pp. 5913-5917 ◽

Cited By ~ 66

Author(s):

N. Mullane ◽

B. Healy ◽

J. Meade ◽

P. Whyte ◽

P. G. Wall ◽

...

Keyword(s):

Milk Protein ◽

Microbial Contamination ◽

Protein Processing ◽

Pulsed Field Gel Electrophoresis ◽

Enterobacter Sakazakii ◽

Powdered Milk ◽

Air Filters ◽

Air Filter ◽

Manufacturing Facility ◽

Processing Facility

ABSTRACT The microbial contamination of air filters and possible links to contaminated product in a powdered milk protein-processing facility were investigated. Over a 10-month period, seven air filters, the environment, and powdered product were analyzed for the presence of Cronobacter spp. The effects of air filter installation, maintenance, and subsequent dissemination of Cronobacter were investigated. A total of 30 isolates were characterized by pulsed-field gel electrophoresis (PFGE). PFGE revealed the presence of three clonal populations distributed throughout the manufacturing site. This study highlights the need for proper installation of air filters to limit the dissemination of microorganisms into processing sites.

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